Details
| Original language | English |
|---|---|
| Article number | 108109 |
| Journal | Soil Biology and Biochemistry |
| Volume | 153 |
| Early online date | 11 Dec 2020 |
| Publication status | Published - Feb 2021 |
Abstract
Microorganisms interact in complex communities, affecting microbially-mediated processes in the environment. Particularly, aerobic methanotrophs showed significantly stimulated growth and activity in the presence of accompanying microorganisms in an interaction network (interactome). Yet, little is known of how the interactome responds to disturbances, and how community functioning is affected by the disturbance-induced structuring of the interaction network. Here, we employed a time-series stable isotope probing (SIP) approach using 13C–CH4 coupled to a co-occurrence network analysis after Illumina MiSeq sequencing of the 13C-enriched 16S rRNA gene to directly relate the response in methanotrophic activity to the network structure of the interactome after desiccation-rewetting of a paddy soil. Methane uptake rate decreased immediately (<5 days) after short-term desiccation-rewetting. Although the methanotroph subgroups differentially responded to desiccation-rewetting, the metabolically active bacterial community composition, including the methanotrophs, recovered after the disturbance. However, the interaction network was profoundly altered, becoming more complex but, less modular after desiccation-rewetting, despite the recovery in the methanotrophic activity and community composition/abundances. This suggests that the legacy of the disturbance persists in the interaction network. The change in the network structure may have consequences for community functioning with recurring desiccation-rewetting.
Keywords
- Community ecology, Methane-based foodweb, Methanotrophs, pmoA, Stable-isotope probing
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology
- Agricultural and Biological Sciences(all)
- Soil Science
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In: Soil Biology and Biochemistry, Vol. 153, 108109, 02.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - When the going gets tough
T2 - Emergence of a complex methane-driven interaction network during recovery from desiccation-rewetting
AU - Kaupper, Thomas
AU - Mendes, Lucas W.
AU - Lee, Hyo Jung
AU - Mo, Yongliang
AU - Poehlein, Anja
AU - Jia, Zhongjun
AU - Horn, Marcus A.
AU - Ho, Adrian
N1 - Funding Information: We are grateful to Stefanie Hetz and Daria Frohloff for excellent research assistance. TK and AH are financially supported by the Deutsche Forschungsgemeinschaft (grant no. HO6234/1-1 ). AH and MAH are also financially supported by the Leibniz Universität Hannover, Germany .
PY - 2021/2
Y1 - 2021/2
N2 - Microorganisms interact in complex communities, affecting microbially-mediated processes in the environment. Particularly, aerobic methanotrophs showed significantly stimulated growth and activity in the presence of accompanying microorganisms in an interaction network (interactome). Yet, little is known of how the interactome responds to disturbances, and how community functioning is affected by the disturbance-induced structuring of the interaction network. Here, we employed a time-series stable isotope probing (SIP) approach using 13C–CH4 coupled to a co-occurrence network analysis after Illumina MiSeq sequencing of the 13C-enriched 16S rRNA gene to directly relate the response in methanotrophic activity to the network structure of the interactome after desiccation-rewetting of a paddy soil. Methane uptake rate decreased immediately (<5 days) after short-term desiccation-rewetting. Although the methanotroph subgroups differentially responded to desiccation-rewetting, the metabolically active bacterial community composition, including the methanotrophs, recovered after the disturbance. However, the interaction network was profoundly altered, becoming more complex but, less modular after desiccation-rewetting, despite the recovery in the methanotrophic activity and community composition/abundances. This suggests that the legacy of the disturbance persists in the interaction network. The change in the network structure may have consequences for community functioning with recurring desiccation-rewetting.
AB - Microorganisms interact in complex communities, affecting microbially-mediated processes in the environment. Particularly, aerobic methanotrophs showed significantly stimulated growth and activity in the presence of accompanying microorganisms in an interaction network (interactome). Yet, little is known of how the interactome responds to disturbances, and how community functioning is affected by the disturbance-induced structuring of the interaction network. Here, we employed a time-series stable isotope probing (SIP) approach using 13C–CH4 coupled to a co-occurrence network analysis after Illumina MiSeq sequencing of the 13C-enriched 16S rRNA gene to directly relate the response in methanotrophic activity to the network structure of the interactome after desiccation-rewetting of a paddy soil. Methane uptake rate decreased immediately (<5 days) after short-term desiccation-rewetting. Although the methanotroph subgroups differentially responded to desiccation-rewetting, the metabolically active bacterial community composition, including the methanotrophs, recovered after the disturbance. However, the interaction network was profoundly altered, becoming more complex but, less modular after desiccation-rewetting, despite the recovery in the methanotrophic activity and community composition/abundances. This suggests that the legacy of the disturbance persists in the interaction network. The change in the network structure may have consequences for community functioning with recurring desiccation-rewetting.
KW - Community ecology
KW - Methane-based foodweb
KW - Methanotrophs
KW - pmoA
KW - Stable-isotope probing
UR - http://www.scopus.com/inward/record.url?scp=85097652808&partnerID=8YFLogxK
U2 - 10.15488/15946
DO - 10.15488/15946
M3 - Article
AN - SCOPUS:85097652808
VL - 153
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
SN - 0038-0717
M1 - 108109
ER -