Details
| Original language | English |
|---|---|
| Pages (from-to) | 686-691 |
| Number of pages | 6 |
| Journal | Journal of Raman spectroscopy |
| Volume | 48 |
| Issue number | 5 |
| Early online date | 17 Feb 2017 |
| Publication status | Published - May 2017 |
Abstract
Measuring the kinetics of the violaxanthin cycle imposes an experimental challenge. Traditionally, carotenoid analysis was carried out laboriously with high-performance liquid chromatography. In this work, we present the first in vivo approach to directly measure the kinetics of the violaxanthin cycle, using resonance Raman spectroscopy in combination with baseline correction and principal component analysis. Applying the new approach allows measuring thousands of data points as opposed to the few possible with chemical analysis over the course of a violaxanthin cycle kinetics experiment. In vivo analysis of the violaxanthin cycle is necessary to fully understand adaptation kinetics to varying light conditions, the knowledge of which is especially important for assessing the stress tolerance of plants in the wake of the increasing climate change. Three experiments on the green alga Dunaliella salina were performed, featuring both the light-to-dark and dark-to-light transitions response of the algae.
Keywords
- Dunaliellasalina, kinetics, resonance Raman, stress adaptation, violaxanthin cycle
ASJC Scopus subject areas
- Materials Science(all)
- Chemistry(all)
- Spectroscopy
Sustainable Development Goals
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In: Journal of Raman spectroscopy, Vol. 48, No. 5, 05.2017, p. 686-691.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Violaxanthin cycle kinetics analysed in vivo with resonance Raman spectroscopy
AU - Koch, Matthias
AU - Zagermann, Serge
AU - Kniggendorf, Ann Kathrin
AU - Meinhardt-Wollweber, Merve
AU - Roth, Bernhard
N1 - Publisher Copyright: Copyright © 2017 John Wiley & Sons, Ltd. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/5
Y1 - 2017/5
N2 - Measuring the kinetics of the violaxanthin cycle imposes an experimental challenge. Traditionally, carotenoid analysis was carried out laboriously with high-performance liquid chromatography. In this work, we present the first in vivo approach to directly measure the kinetics of the violaxanthin cycle, using resonance Raman spectroscopy in combination with baseline correction and principal component analysis. Applying the new approach allows measuring thousands of data points as opposed to the few possible with chemical analysis over the course of a violaxanthin cycle kinetics experiment. In vivo analysis of the violaxanthin cycle is necessary to fully understand adaptation kinetics to varying light conditions, the knowledge of which is especially important for assessing the stress tolerance of plants in the wake of the increasing climate change. Three experiments on the green alga Dunaliella salina were performed, featuring both the light-to-dark and dark-to-light transitions response of the algae.
AB - Measuring the kinetics of the violaxanthin cycle imposes an experimental challenge. Traditionally, carotenoid analysis was carried out laboriously with high-performance liquid chromatography. In this work, we present the first in vivo approach to directly measure the kinetics of the violaxanthin cycle, using resonance Raman spectroscopy in combination with baseline correction and principal component analysis. Applying the new approach allows measuring thousands of data points as opposed to the few possible with chemical analysis over the course of a violaxanthin cycle kinetics experiment. In vivo analysis of the violaxanthin cycle is necessary to fully understand adaptation kinetics to varying light conditions, the knowledge of which is especially important for assessing the stress tolerance of plants in the wake of the increasing climate change. Three experiments on the green alga Dunaliella salina were performed, featuring both the light-to-dark and dark-to-light transitions response of the algae.
KW - Dunaliellasalina
KW - kinetics
KW - resonance Raman
KW - stress adaptation
KW - violaxanthin cycle
UR - http://www.scopus.com/inward/record.url?scp=85013439647&partnerID=8YFLogxK
U2 - 10.15488/1214
DO - 10.15488/1214
M3 - Article
AN - SCOPUS:85013439647
VL - 48
SP - 686
EP - 691
JO - Journal of Raman spectroscopy
JF - Journal of Raman spectroscopy
SN - 0377-0486
IS - 5
ER -