Details
| Original language | English |
|---|---|
| Pages (from-to) | 204-209 |
| Number of pages | 6 |
| Journal | Propagation of Ornamental Plants |
| Volume | 8 |
| Issue number | 4 |
| Publication status | Published - Dec 2008 |
Abstract
While Cyclamen persicum Miller has gained economic importance as an important ornamental plant worldwide, only a few of the other nineteen species of the same genus have commercial relevance although having a lot of interesting and valuable traits. To examine these traits under in vitro culture and greenhouse conditions an efficient in vitro propagation method of different Cyclamen species was established. Sterile seedlings of Cyclamen mirabile Hildebrand, Cyclamen coum Miller, Cyclamen graecum Link and Cyclamen hederifolium Aiton divided in cotyledon, tuber and roots were used as a starting material. Shoot induction was obtained on half-strength Murashige and Skoog based medium containing 0.5 mg 1-1 NAA and 1 mg 1-1 BAP and on medium with 0.5 mg 1-1 IAA, 1 mg 1-1 BAP, 1 mg 1-1 2iP and 1 mg 1-1 kinetin as growth regulators. The most suitable tissues for shoot induction were tubers and/or cotyledons depending on the species. Less or no shoots were obtained from roots when used as starting tissue, but root formation was found in high frequency. There were pronounced genotypic differences in the reaction to the different media within one species. The medium with lower contents of cytokinins showed high formation of roots, possibly due to high endogenous auxin content in the tissue.
Keywords
- Adventitious shoot induction, Cyclamen, In vitro propagation, Organogenesis, Ornamental plant, Regeneration
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Forestry
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Propagation of Ornamental Plants, Vol. 8, No. 4, 12.2008, p. 204-209.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Vegetative propagation of different cyclamen species via adventitious shoot formation from seedling tissue
AU - Prange, Anika Nadja Sabine
AU - Serek, Margrethe
AU - Winkelmann, Traud
PY - 2008/12
Y1 - 2008/12
N2 - While Cyclamen persicum Miller has gained economic importance as an important ornamental plant worldwide, only a few of the other nineteen species of the same genus have commercial relevance although having a lot of interesting and valuable traits. To examine these traits under in vitro culture and greenhouse conditions an efficient in vitro propagation method of different Cyclamen species was established. Sterile seedlings of Cyclamen mirabile Hildebrand, Cyclamen coum Miller, Cyclamen graecum Link and Cyclamen hederifolium Aiton divided in cotyledon, tuber and roots were used as a starting material. Shoot induction was obtained on half-strength Murashige and Skoog based medium containing 0.5 mg 1-1 NAA and 1 mg 1-1 BAP and on medium with 0.5 mg 1-1 IAA, 1 mg 1-1 BAP, 1 mg 1-1 2iP and 1 mg 1-1 kinetin as growth regulators. The most suitable tissues for shoot induction were tubers and/or cotyledons depending on the species. Less or no shoots were obtained from roots when used as starting tissue, but root formation was found in high frequency. There were pronounced genotypic differences in the reaction to the different media within one species. The medium with lower contents of cytokinins showed high formation of roots, possibly due to high endogenous auxin content in the tissue.
AB - While Cyclamen persicum Miller has gained economic importance as an important ornamental plant worldwide, only a few of the other nineteen species of the same genus have commercial relevance although having a lot of interesting and valuable traits. To examine these traits under in vitro culture and greenhouse conditions an efficient in vitro propagation method of different Cyclamen species was established. Sterile seedlings of Cyclamen mirabile Hildebrand, Cyclamen coum Miller, Cyclamen graecum Link and Cyclamen hederifolium Aiton divided in cotyledon, tuber and roots were used as a starting material. Shoot induction was obtained on half-strength Murashige and Skoog based medium containing 0.5 mg 1-1 NAA and 1 mg 1-1 BAP and on medium with 0.5 mg 1-1 IAA, 1 mg 1-1 BAP, 1 mg 1-1 2iP and 1 mg 1-1 kinetin as growth regulators. The most suitable tissues for shoot induction were tubers and/or cotyledons depending on the species. Less or no shoots were obtained from roots when used as starting tissue, but root formation was found in high frequency. There were pronounced genotypic differences in the reaction to the different media within one species. The medium with lower contents of cytokinins showed high formation of roots, possibly due to high endogenous auxin content in the tissue.
KW - Adventitious shoot induction
KW - Cyclamen
KW - In vitro propagation
KW - Organogenesis
KW - Ornamental plant
KW - Regeneration
UR - http://www.scopus.com/inward/record.url?scp=56049092775&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:56049092775
VL - 8
SP - 204
EP - 209
JO - Propagation of Ornamental Plants
JF - Propagation of Ornamental Plants
SN - 1311-9109
IS - 4
ER -