Details
| Original language | English |
|---|---|
| Pages (from-to) | 1239-1249 |
| Number of pages | 11 |
| Journal | Tissue Engineering - Part A |
| Volume | 27 |
| Issue number | 19-20 |
| Early online date | 24 Feb 2021 |
| Publication status | Published - 18 Oct 2021 |
Abstract
In this study, microvascular network structures for tissue engineering were generated on newly developed macroporous polydioxanone (PDO) scaffolds. PDO represents a polymer biodegradable within months and offers optimal material properties such as elasticity and nontoxic degradation products. PDO scaffolds prepared by porogen leaching and cryo-dried to achieve pore sizes of 326 ± 149.67 μm remained stable with equivalent values for Young's modulus after 4 weeks. Scaffolds were coated with fibrin for optimal cell adherence. To exclude interindividual differences, autologous fibrin was prepared out of human plasma-derived fibrinogen and proved a comparable quality to nonautologous commercially available fibrinogen. Fibrin-coated scaffolds were seeded with recombinant human umbilical vein endothelial cells expressing GFP (GFP-HUVECs) in coculture with adipose tissue-derived mesenchymal stem cells (AD-hMSCs) to form vascular networks. The growth factor content in culture media was optimized according its effect on network formation, quantified and assessed by AngioTool®. A ratio of 2:3 GFP-HUVECs/AD-hMSCs in medium enriched with 20 ng/mL vascular endothelial growth factor, basic fibroblast growth factor, and hydrocortisone was found to be optimal. Network structures appeared after 2 days of cultivation and stabilized until day 7. The resulting networks were lumenized that could be verified by dextran staining. This new approach might be suitable for microvascular tissue patches as a useful template to be used in diverse vascularized tissue constructs.
Keywords
- angiogenesis, fibrin, polydioxanone, scaffolds, vascular networks, VEGF
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Engineering(all)
- Biomedical Engineering
- Materials Science(all)
- Biomaterials
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In: Tissue Engineering - Part A, Vol. 27, No. 19-20, 18.10.2021, p. 1239-1249.
Research output: Contribution to journal › Article › Research
}
TY - JOUR
T1 - Vascular Network Formation on Macroporous Polydioxanone Scaffolds
AU - Heene, Sebastian
AU - Thoms, Stefanie
AU - Kalies, Stefan
AU - Wegner, Nils
AU - Peppermüller, Pia
AU - Born, Nikolai
AU - Walther, Frank
AU - Scheper, Thomas
AU - Blume, Cornelia A
N1 - Funding Information: This study was funded by the Deutsche Forschungs-gemeinschaft (DFG;Grant No. 388094931). This work has further been carried out within the framework of the SMART BIOTECS alliance between the Technische Universitaet Braunschweig and the Leibniz Universitaet Hannover. This initiative is supported by the Ministry of Economy and Culture (MWK) of Lower Saxony, Germany.
PY - 2021/10/18
Y1 - 2021/10/18
N2 - In this study, microvascular network structures for tissue engineering were generated on newly developed macroporous polydioxanone (PDO) scaffolds. PDO represents a polymer biodegradable within months and offers optimal material properties such as elasticity and nontoxic degradation products. PDO scaffolds prepared by porogen leaching and cryo-dried to achieve pore sizes of 326 ± 149.67 μm remained stable with equivalent values for Young's modulus after 4 weeks. Scaffolds were coated with fibrin for optimal cell adherence. To exclude interindividual differences, autologous fibrin was prepared out of human plasma-derived fibrinogen and proved a comparable quality to nonautologous commercially available fibrinogen. Fibrin-coated scaffolds were seeded with recombinant human umbilical vein endothelial cells expressing GFP (GFP-HUVECs) in coculture with adipose tissue-derived mesenchymal stem cells (AD-hMSCs) to form vascular networks. The growth factor content in culture media was optimized according its effect on network formation, quantified and assessed by AngioTool®. A ratio of 2:3 GFP-HUVECs/AD-hMSCs in medium enriched with 20 ng/mL vascular endothelial growth factor, basic fibroblast growth factor, and hydrocortisone was found to be optimal. Network structures appeared after 2 days of cultivation and stabilized until day 7. The resulting networks were lumenized that could be verified by dextran staining. This new approach might be suitable for microvascular tissue patches as a useful template to be used in diverse vascularized tissue constructs.
AB - In this study, microvascular network structures for tissue engineering were generated on newly developed macroporous polydioxanone (PDO) scaffolds. PDO represents a polymer biodegradable within months and offers optimal material properties such as elasticity and nontoxic degradation products. PDO scaffolds prepared by porogen leaching and cryo-dried to achieve pore sizes of 326 ± 149.67 μm remained stable with equivalent values for Young's modulus after 4 weeks. Scaffolds were coated with fibrin for optimal cell adherence. To exclude interindividual differences, autologous fibrin was prepared out of human plasma-derived fibrinogen and proved a comparable quality to nonautologous commercially available fibrinogen. Fibrin-coated scaffolds were seeded with recombinant human umbilical vein endothelial cells expressing GFP (GFP-HUVECs) in coculture with adipose tissue-derived mesenchymal stem cells (AD-hMSCs) to form vascular networks. The growth factor content in culture media was optimized according its effect on network formation, quantified and assessed by AngioTool®. A ratio of 2:3 GFP-HUVECs/AD-hMSCs in medium enriched with 20 ng/mL vascular endothelial growth factor, basic fibroblast growth factor, and hydrocortisone was found to be optimal. Network structures appeared after 2 days of cultivation and stabilized until day 7. The resulting networks were lumenized that could be verified by dextran staining. This new approach might be suitable for microvascular tissue patches as a useful template to be used in diverse vascularized tissue constructs.
KW - angiogenesis
KW - fibrin
KW - polydioxanone
KW - scaffolds
KW - vascular networks
KW - VEGF
UR - http://www.scopus.com/inward/record.url?scp=85118245902&partnerID=8YFLogxK
U2 - 10.1089/ten.TEA.2020.0232
DO - 10.1089/ten.TEA.2020.0232
M3 - Article
C2 - 33397206
VL - 27
SP - 1239
EP - 1249
JO - Tissue Engineering - Part A
JF - Tissue Engineering - Part A
SN - 1937-3341
IS - 19-20
ER -