Details
| Original language | English |
|---|---|
| Pages (from-to) | 243-248 |
| Number of pages | 6 |
| Journal | Journal of general plant pathology |
| Volume | 70 |
| Issue number | 4 |
| Publication status | Published - Aug 2004 |
Abstract
The molecular variability in the DNA-A of cassava-begomoviruses, East African cassava mosaic virus (EACMV), East African cassava mosaic virus - Uganda variant (EACMV-UG), African cassava mosaic virus (ACMV), and East African cassava mosaic Zanzibar virus (EACMZV) in Kenya, Uganda, and Democratic Republic of the Congo (DRC) was investigated. Most samples from western Kenya, Uganda, and eastern DRC contained EACMV-UG. Fewer than half of the samples had a mixed infection of ACMV and EACMV-UG, and a small percentage had only ACMV infections. EACMV and EACMZV were the only begomoviruses detected in samples from the Kenyan coast. The coat protein gene nucleotide (nt) and the deduced amino acid sequence analyses revealed a high degree of sequence identity within each virus type and that EACMV-UG was most related to ACMV. However, analysis of the retrieved complete DNA-A (2781-2801 nt) sequences of selected virus types revealed that EACMV-UG DNA-A share more than 90% identity with EACMV and less than 80% with ACMV, confirming that the virus is a strain of EACMV.
Keywords
- Begomovirus, Cassava, Distribution, EACMV-UG, Taxonomic status
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Journal of general plant pathology, Vol. 70, No. 4, 08.2004, p. 243-248.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Variations and taxonomic status of begomoviruses causing severe epidemics of cassava mosaic disease in Kenya, Uganda, and Democratic Republic of the Congo
AU - Were, Hassan Karakacha
AU - Winter, Stephan
AU - Maiss, Edgar
N1 - Funding Information: Acknowledgments We sincerely thank Prof. Dr. Y. Takanami and Dr. Lina Katul for their useful discussions and advice. We also thank Prof. Dr. P.O. Ayiecho for his guidance during sample collection in Kenya and Mr. Pierre E. Bolonge for providing infected cassava samples from the DRC. This work was funded in part by the German Academic Exchange Service (DAAD).
PY - 2004/8
Y1 - 2004/8
N2 - The molecular variability in the DNA-A of cassava-begomoviruses, East African cassava mosaic virus (EACMV), East African cassava mosaic virus - Uganda variant (EACMV-UG), African cassava mosaic virus (ACMV), and East African cassava mosaic Zanzibar virus (EACMZV) in Kenya, Uganda, and Democratic Republic of the Congo (DRC) was investigated. Most samples from western Kenya, Uganda, and eastern DRC contained EACMV-UG. Fewer than half of the samples had a mixed infection of ACMV and EACMV-UG, and a small percentage had only ACMV infections. EACMV and EACMZV were the only begomoviruses detected in samples from the Kenyan coast. The coat protein gene nucleotide (nt) and the deduced amino acid sequence analyses revealed a high degree of sequence identity within each virus type and that EACMV-UG was most related to ACMV. However, analysis of the retrieved complete DNA-A (2781-2801 nt) sequences of selected virus types revealed that EACMV-UG DNA-A share more than 90% identity with EACMV and less than 80% with ACMV, confirming that the virus is a strain of EACMV.
AB - The molecular variability in the DNA-A of cassava-begomoviruses, East African cassava mosaic virus (EACMV), East African cassava mosaic virus - Uganda variant (EACMV-UG), African cassava mosaic virus (ACMV), and East African cassava mosaic Zanzibar virus (EACMZV) in Kenya, Uganda, and Democratic Republic of the Congo (DRC) was investigated. Most samples from western Kenya, Uganda, and eastern DRC contained EACMV-UG. Fewer than half of the samples had a mixed infection of ACMV and EACMV-UG, and a small percentage had only ACMV infections. EACMV and EACMZV were the only begomoviruses detected in samples from the Kenyan coast. The coat protein gene nucleotide (nt) and the deduced amino acid sequence analyses revealed a high degree of sequence identity within each virus type and that EACMV-UG was most related to ACMV. However, analysis of the retrieved complete DNA-A (2781-2801 nt) sequences of selected virus types revealed that EACMV-UG DNA-A share more than 90% identity with EACMV and less than 80% with ACMV, confirming that the virus is a strain of EACMV.
KW - Begomovirus
KW - Cassava
KW - Distribution
KW - EACMV-UG
KW - Taxonomic status
UR - http://www.scopus.com/inward/record.url?scp=4344676335&partnerID=8YFLogxK
U2 - 10.1007/s10327-003-0119-y
DO - 10.1007/s10327-003-0119-y
M3 - Article
AN - SCOPUS:4344676335
VL - 70
SP - 243
EP - 248
JO - Journal of general plant pathology
JF - Journal of general plant pathology
SN - 1345-2630
IS - 4
ER -