Details
| Original language | English |
|---|---|
| Pages (from-to) | 160-166 |
| Number of pages | 7 |
| Journal | Journal of plant physiology |
| Volume | 150 |
| Issue number | 1-2 |
| Publication status | Published - 1997 |
Abstract
Callus cultures of Coleonema album were grown under different light regimes and in the presence of different concentrations of phytoeffectors and gelling agents. Both an increased photonic fluence and photoperiod stimulated the accumulation of chlorophyll (max 450 mg per kg wet weight) and of volatile monoterpenes. The application of different light sources showed that chlorophyll and terpene biosynthesis (max 130 mg per kg wet weight) were stably correlated during 11 subcultivations. Phytoeffector concentrations modulated some of the light effects: Formation of chlorophyll and aliphatic esters occurred in a broad range of phytoeffector conditions, while volatile terpenes were restricted to a narrow window. Increased concentrations of agar or gellan gum (≤12 g L-1 decreased growth and increased concentrations of chlorophyll and volatiles. Photomixotrophic cells are discussed as an in vitro system to study the control of oligoisoprenoid formation in higher plants.
Keywords
- chlorophyll, Coleonema album, gelling agent, light, photomixotrophy, plant cell culture, volatile terpenes
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Journal of plant physiology, Vol. 150, No. 1-2, 1997, p. 160-166.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Variation of chlorophyll and essential oils in photomixotrophic cell cultures of Coleonema album (Thunb.)
AU - Reil, G.
AU - Berger, R. G.
N1 - Funding information: This work was supported by the Federal Minister of Research and Technology (no. 0318980A) and by the Fonds der Chemischen Industrie. Valuable advice came from the members of the project group. S. Pilawa and B. Zimmermann are thanked for analytical and technical collaboration.
PY - 1997
Y1 - 1997
N2 - Callus cultures of Coleonema album were grown under different light regimes and in the presence of different concentrations of phytoeffectors and gelling agents. Both an increased photonic fluence and photoperiod stimulated the accumulation of chlorophyll (max 450 mg per kg wet weight) and of volatile monoterpenes. The application of different light sources showed that chlorophyll and terpene biosynthesis (max 130 mg per kg wet weight) were stably correlated during 11 subcultivations. Phytoeffector concentrations modulated some of the light effects: Formation of chlorophyll and aliphatic esters occurred in a broad range of phytoeffector conditions, while volatile terpenes were restricted to a narrow window. Increased concentrations of agar or gellan gum (≤12 g L-1 decreased growth and increased concentrations of chlorophyll and volatiles. Photomixotrophic cells are discussed as an in vitro system to study the control of oligoisoprenoid formation in higher plants.
AB - Callus cultures of Coleonema album were grown under different light regimes and in the presence of different concentrations of phytoeffectors and gelling agents. Both an increased photonic fluence and photoperiod stimulated the accumulation of chlorophyll (max 450 mg per kg wet weight) and of volatile monoterpenes. The application of different light sources showed that chlorophyll and terpene biosynthesis (max 130 mg per kg wet weight) were stably correlated during 11 subcultivations. Phytoeffector concentrations modulated some of the light effects: Formation of chlorophyll and aliphatic esters occurred in a broad range of phytoeffector conditions, while volatile terpenes were restricted to a narrow window. Increased concentrations of agar or gellan gum (≤12 g L-1 decreased growth and increased concentrations of chlorophyll and volatiles. Photomixotrophic cells are discussed as an in vitro system to study the control of oligoisoprenoid formation in higher plants.
KW - chlorophyll
KW - Coleonema album
KW - gelling agent
KW - light
KW - photomixotrophy
KW - plant cell culture
KW - volatile terpenes
UR - http://www.scopus.com/inward/record.url?scp=0030937152&partnerID=8YFLogxK
U2 - 10.1016/S0176-1617(97)80196-4
DO - 10.1016/S0176-1617(97)80196-4
M3 - Article
AN - SCOPUS:0030937152
VL - 150
SP - 160
EP - 166
JO - Journal of plant physiology
JF - Journal of plant physiology
SN - 0176-1617
IS - 1-2
ER -