Details
| Original language | English |
|---|---|
| Pages (from-to) | 255-263 |
| Number of pages | 9 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 90 |
| Issue number | 3 |
| Publication status | Published - 21 Jul 2007 |
Abstract
By flow cytometric experiments in Cyclamen persicum both with propidium iodide (PI) and DAPI (4′,6-Diamidino-2-phenylindol)-staining we were able to present (1) a new estimation for the absolute DNA content in the range of 3.17 pg DNA/2C and (2) ploidy abnormalities which were detected in the pathway of somatic embryogenesis. These aberrations might have arisen from ploidy mutations or abnormal polyploidization processes. Morphologically normal somatic embryos contained the smallest proportion of individuals with ploidy abnormalities, but also a regenerated plant in the green house displayed a significantly elevated DNA content. The results are discussed in the framework of future application of in vitro propagation techniques based on regeneration from fastly growing undifferentiated cells like long-term callus and suspension cultures.
Keywords
- DNA amount, DNA-Index, Flow cytometry, Micropropagation, Somaclonal variation
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Plant Cell, Tissue and Organ Culture, Vol. 90, No. 3, 21.07.2007, p. 255-263.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Variable DNA content of Cyclamen persicum regenerated via somatic embryogenesis
T2 - Rethinking the concept of long-term callus and suspension cultures
AU - Borchert, Thomas
AU - Fuchs, Jörg
AU - Winkelmann, Traud
AU - Hohe, Annette
PY - 2007/7/21
Y1 - 2007/7/21
N2 - By flow cytometric experiments in Cyclamen persicum both with propidium iodide (PI) and DAPI (4′,6-Diamidino-2-phenylindol)-staining we were able to present (1) a new estimation for the absolute DNA content in the range of 3.17 pg DNA/2C and (2) ploidy abnormalities which were detected in the pathway of somatic embryogenesis. These aberrations might have arisen from ploidy mutations or abnormal polyploidization processes. Morphologically normal somatic embryos contained the smallest proportion of individuals with ploidy abnormalities, but also a regenerated plant in the green house displayed a significantly elevated DNA content. The results are discussed in the framework of future application of in vitro propagation techniques based on regeneration from fastly growing undifferentiated cells like long-term callus and suspension cultures.
AB - By flow cytometric experiments in Cyclamen persicum both with propidium iodide (PI) and DAPI (4′,6-Diamidino-2-phenylindol)-staining we were able to present (1) a new estimation for the absolute DNA content in the range of 3.17 pg DNA/2C and (2) ploidy abnormalities which were detected in the pathway of somatic embryogenesis. These aberrations might have arisen from ploidy mutations or abnormal polyploidization processes. Morphologically normal somatic embryos contained the smallest proportion of individuals with ploidy abnormalities, but also a regenerated plant in the green house displayed a significantly elevated DNA content. The results are discussed in the framework of future application of in vitro propagation techniques based on regeneration from fastly growing undifferentiated cells like long-term callus and suspension cultures.
KW - DNA amount
KW - DNA-Index
KW - Flow cytometry
KW - Micropropagation
KW - Somaclonal variation
UR - http://www.scopus.com/inward/record.url?scp=34547839658&partnerID=8YFLogxK
U2 - 10.1007/s11240-007-9264-x
DO - 10.1007/s11240-007-9264-x
M3 - Article
AN - SCOPUS:34547839658
VL - 90
SP - 255
EP - 263
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 3
ER -