Details
| Original language | English |
|---|---|
| Pages (from-to) | 207-215 |
| Number of pages | 9 |
| Journal | Propagation of Ornamental Plants |
| Volume | 9 |
| Issue number | 4 |
| Publication status | Published - 2009 |
Abstract
Traditional selection markers used for production of transgenic plants, such as antibiotic resistance, are not acceptable for commercial registration in some countries. Mannose is a promising alternative selective agent, adapted to Agrobacterium tumefaciens-mediated transformation, and regeneration of Kalanchoe blossfeldiana 'Molly' and breeding line '1998-469'. Leaf explants were selected on MS medium supplemented with different concentrations and combinations of mannose or/and glucose to establish an effective mannose selection protocol. Mannose at 20 g l-1 was the most efficient concentration for eliminating non-transgenic shoots, while mannose at 35-45 g l-1 in MS medium inhibited shoot growth and negatively influenced explant regeneration. A GUS assay confirmed expression of the uidA gene in transgenic plants. Transformation efficiency rate ranged 0-15% for 'Molly' and 6.7-39% for breeding line '1998-469'. The results demonstrated that phosphomannose isomerase (PMI) was an effective selective marker for transformation of K. blossfeldiana.
Keywords
- Agrobacterium tumefaciens, Phosphomannose isomerase, Selective agent
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Forestry
- Agricultural and Biological Sciences(all)
- Plant Science
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Propagation of Ornamental Plants, Vol. 9, No. 4, 2009, p. 207-215.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Use of the PMI/mannose selection system for genetic transformation of Kalanchoe blossfeldiana poelln
AU - Ilczuk, Agnieszka
AU - Mibus, Heiko
AU - Schmidt, Malthe
AU - Serek, Margrethe
PY - 2009
Y1 - 2009
N2 - Traditional selection markers used for production of transgenic plants, such as antibiotic resistance, are not acceptable for commercial registration in some countries. Mannose is a promising alternative selective agent, adapted to Agrobacterium tumefaciens-mediated transformation, and regeneration of Kalanchoe blossfeldiana 'Molly' and breeding line '1998-469'. Leaf explants were selected on MS medium supplemented with different concentrations and combinations of mannose or/and glucose to establish an effective mannose selection protocol. Mannose at 20 g l-1 was the most efficient concentration for eliminating non-transgenic shoots, while mannose at 35-45 g l-1 in MS medium inhibited shoot growth and negatively influenced explant regeneration. A GUS assay confirmed expression of the uidA gene in transgenic plants. Transformation efficiency rate ranged 0-15% for 'Molly' and 6.7-39% for breeding line '1998-469'. The results demonstrated that phosphomannose isomerase (PMI) was an effective selective marker for transformation of K. blossfeldiana.
AB - Traditional selection markers used for production of transgenic plants, such as antibiotic resistance, are not acceptable for commercial registration in some countries. Mannose is a promising alternative selective agent, adapted to Agrobacterium tumefaciens-mediated transformation, and regeneration of Kalanchoe blossfeldiana 'Molly' and breeding line '1998-469'. Leaf explants were selected on MS medium supplemented with different concentrations and combinations of mannose or/and glucose to establish an effective mannose selection protocol. Mannose at 20 g l-1 was the most efficient concentration for eliminating non-transgenic shoots, while mannose at 35-45 g l-1 in MS medium inhibited shoot growth and negatively influenced explant regeneration. A GUS assay confirmed expression of the uidA gene in transgenic plants. Transformation efficiency rate ranged 0-15% for 'Molly' and 6.7-39% for breeding line '1998-469'. The results demonstrated that phosphomannose isomerase (PMI) was an effective selective marker for transformation of K. blossfeldiana.
KW - Agrobacterium tumefaciens
KW - Phosphomannose isomerase
KW - Selective agent
UR - http://www.scopus.com/inward/record.url?scp=73549104600&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:73549104600
VL - 9
SP - 207
EP - 215
JO - Propagation of Ornamental Plants
JF - Propagation of Ornamental Plants
SN - 1311-9109
IS - 4
ER -