Details
| Original language | English |
|---|---|
| Article number | 12982 |
| Journal | Scientific reports |
| Volume | 8 |
| Publication status | Published - 28 Aug 2018 |
Abstract
Freeze-dried storage of decellularized heart valves provides easy storage and transport for clinical use. Freeze-drying without protectants, however, results in a disrupted histoarchitecture after rehydration. In this study, heart valves were incubated in solutions of various sucrose concentrations and subsequently freeze-dried. Porosity of rehydrated valves was determined from histological images. In the absence of sucrose, freeze-dried valves were shown to have pores after rehydration in the cusp, artery and muscle sections. Use of sucrose reduced pore formation in a dose-dependent manner, and pretreatment of the valves in a 40% (w/v) sucrose solution prior to freeze-drying was found to be sufficient to completely diminish pore formation. The presence of pores in freeze-dried valves was found to coincide with altered biomechanical characteristics, whereas biomechanical parameters of valves freeze-dried with enough sucrose were not significantly different from those of valves not exposed to freeze-drying. Multiphoton imaging, Fourier transform infrared spectroscopy, and differential scanning calorimetry studies revealed that matrix proteins (i.e. collagen and elastin) were not affected by freeze-drying.
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In: Scientific reports, Vol. 8, 12982, 28.08.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Use of sucrose to diminish pore formation in freeze-dried heart valves
AU - Vásquez-Rivera, Andrés
AU - Oldenhof, Harriëtte
AU - Dipresa, Daniele
AU - Goecke, Tobias
AU - Kouvaka, Artemis
AU - Will, Fabian
AU - Haverich, Axel
AU - Korossis, Sotirios
AU - Hilfiker, Andres
AU - Wolkers, Willem F.
N1 - Funding Information: This research was supported by the German Research Foundation (DFG: Deutsche Forschungsgemeinschaft) via the Cluster of Excellence ‘From regenerative biology to reconstructive therapy’ (REBIRTH, EXC 62/1). The publication of this article was funded by the Open Access Fund of the Leibniz Universität Hannover.
PY - 2018/8/28
Y1 - 2018/8/28
N2 - Freeze-dried storage of decellularized heart valves provides easy storage and transport for clinical use. Freeze-drying without protectants, however, results in a disrupted histoarchitecture after rehydration. In this study, heart valves were incubated in solutions of various sucrose concentrations and subsequently freeze-dried. Porosity of rehydrated valves was determined from histological images. In the absence of sucrose, freeze-dried valves were shown to have pores after rehydration in the cusp, artery and muscle sections. Use of sucrose reduced pore formation in a dose-dependent manner, and pretreatment of the valves in a 40% (w/v) sucrose solution prior to freeze-drying was found to be sufficient to completely diminish pore formation. The presence of pores in freeze-dried valves was found to coincide with altered biomechanical characteristics, whereas biomechanical parameters of valves freeze-dried with enough sucrose were not significantly different from those of valves not exposed to freeze-drying. Multiphoton imaging, Fourier transform infrared spectroscopy, and differential scanning calorimetry studies revealed that matrix proteins (i.e. collagen and elastin) were not affected by freeze-drying.
AB - Freeze-dried storage of decellularized heart valves provides easy storage and transport for clinical use. Freeze-drying without protectants, however, results in a disrupted histoarchitecture after rehydration. In this study, heart valves were incubated in solutions of various sucrose concentrations and subsequently freeze-dried. Porosity of rehydrated valves was determined from histological images. In the absence of sucrose, freeze-dried valves were shown to have pores after rehydration in the cusp, artery and muscle sections. Use of sucrose reduced pore formation in a dose-dependent manner, and pretreatment of the valves in a 40% (w/v) sucrose solution prior to freeze-drying was found to be sufficient to completely diminish pore formation. The presence of pores in freeze-dried valves was found to coincide with altered biomechanical characteristics, whereas biomechanical parameters of valves freeze-dried with enough sucrose were not significantly different from those of valves not exposed to freeze-drying. Multiphoton imaging, Fourier transform infrared spectroscopy, and differential scanning calorimetry studies revealed that matrix proteins (i.e. collagen and elastin) were not affected by freeze-drying.
UR - http://www.scopus.com/inward/record.url?scp=85052405756&partnerID=8YFLogxK
U2 - 10.1038/s41598-018-31388-4
DO - 10.1038/s41598-018-31388-4
M3 - Article
C2 - 30154529
AN - SCOPUS:85052405756
VL - 8
JO - Scientific reports
JF - Scientific reports
SN - 2045-2322
M1 - 12982
ER -