Details
| Translated title of the contribution | Differentiation of two thyme deliveries for its use as spices by molecular marker analysis |
|---|---|
| Original language | German |
| Pages (from-to) | 94-98 |
| Number of pages | 5 |
| Journal | Zeitschrift fur Arznei- und Gewurzpflanzen |
| Volume | 19 |
| Issue number | 2 |
| Publication status | Published - Jun 2014 |
Abstract
The identity of a thyme delivery, imported for use in spice mixtures, was doubtful, since smell and consistency did not coincide with the characteristics of former supplies. Therefore, there was the assumption that plant material of a wrong genotype or species had been delivered. With conventional methods (microscopy) the identity of the species could not be clearly determined, an alternative molecular method should be developed. After extraction of the genomic DNA Internal Transcribed Spacer (ITS) sequences were amplified by PCR, sequenced, aligned with known sequences from Genbank and finally a phylogenetic tree was inferred by the Neighbor Joining method. It was clearly demonstrated that Thymus saturejoides was delivered from Egypt instead of Thymus vulgaris whereas the identity of the material from Morocco was clearly analyzed as Thymus vulgaris. With relatively little effort, the critical thyme sample could be unambiguously determined and classified. This method is also applicable to other aromatic plants.
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Food Science
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Zeitschrift fur Arznei- und Gewurzpflanzen, Vol. 19, No. 2, 06.2014, p. 94-98.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Unterscheidung von zwei Thymianlieferungen für die Verwendung als Gewürz mittels molekularer Markeranalyse
AU - Von Trzebiatowski, P.
AU - Rohn, E.
AU - Nguyen, V. X.
AU - Richter, O.
AU - Papenbrock, J.
PY - 2014/6
Y1 - 2014/6
N2 - The identity of a thyme delivery, imported for use in spice mixtures, was doubtful, since smell and consistency did not coincide with the characteristics of former supplies. Therefore, there was the assumption that plant material of a wrong genotype or species had been delivered. With conventional methods (microscopy) the identity of the species could not be clearly determined, an alternative molecular method should be developed. After extraction of the genomic DNA Internal Transcribed Spacer (ITS) sequences were amplified by PCR, sequenced, aligned with known sequences from Genbank and finally a phylogenetic tree was inferred by the Neighbor Joining method. It was clearly demonstrated that Thymus saturejoides was delivered from Egypt instead of Thymus vulgaris whereas the identity of the material from Morocco was clearly analyzed as Thymus vulgaris. With relatively little effort, the critical thyme sample could be unambiguously determined and classified. This method is also applicable to other aromatic plants.
AB - The identity of a thyme delivery, imported for use in spice mixtures, was doubtful, since smell and consistency did not coincide with the characteristics of former supplies. Therefore, there was the assumption that plant material of a wrong genotype or species had been delivered. With conventional methods (microscopy) the identity of the species could not be clearly determined, an alternative molecular method should be developed. After extraction of the genomic DNA Internal Transcribed Spacer (ITS) sequences were amplified by PCR, sequenced, aligned with known sequences from Genbank and finally a phylogenetic tree was inferred by the Neighbor Joining method. It was clearly demonstrated that Thymus saturejoides was delivered from Egypt instead of Thymus vulgaris whereas the identity of the material from Morocco was clearly analyzed as Thymus vulgaris. With relatively little effort, the critical thyme sample could be unambiguously determined and classified. This method is also applicable to other aromatic plants.
KW - Internal transcribed spacer (ITS)
KW - Molecular markers
KW - Thymus saturejoides
KW - Thymus vulgaris
UR - http://www.scopus.com/inward/record.url?scp=84903277941&partnerID=8YFLogxK
M3 - Artikel
AN - SCOPUS:84903277941
VL - 19
SP - 94
EP - 98
JO - Zeitschrift fur Arznei- und Gewurzpflanzen
JF - Zeitschrift fur Arznei- und Gewurzpflanzen
SN - 1431-9292
IS - 2
ER -