Details
| Original language | English |
|---|---|
| Pages (from-to) | 6496-6502 |
| Journal | Catalysis science & technology |
| Volume | 14 |
| Issue number | 22 |
| Early online date | 14 Oct 2024 |
| Publication status | Published - 2024 |
Abstract
In the context of empowering biocatalysis, an easy-to-develop, reproducible, and easy-to-scale biocatalytic system under continuous flow is demonstrated. 3D printing technology is used as the reactor manufacturing method, yielding identical and low-cost microfluidic chips that can be further modified to serve as a biocatalytic platform for simultaneous parameter screening. The model enzyme studied here is unspecific peroxygenase (UPO). UPOs are currently under intensive study, due to their distinct promiscuity in oxyfunctionalization chemistry. This is the first study demonstrating UPO's immobilization in a microfluidic concept. The developed method for surface functionalization of microfluidic reactors is based on polydopamine modification and was proven highly reproducible. UPO showed a TTN of 19 249 and a STY of 2.1 g L -1 h -1, under the specified conditions. The kinetic behavior of the system under flow conditions is reported. The system was also regenerated with a 51.4% recovered activity. Further utilization of microfluidic concepts is expected to unravel the full potential of UPOs for oxyfunctionalization reactions of particular interest. The proposed system is foreseen as a screening platform for different reaction conditions, reaction substrates, or enzyme mutants.
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In: Catalysis science & technology, Vol. 14, No. 22, 2024, p. 6496-6502.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Unspecific peroxygenase immobilization in 3D-printed microfluidics
T2 - towards tailor-made screening platforms
AU - Gkantzou, Elena
AU - Koulopoulou, Theofilia
AU - Brass, Hannah
AU - Schönauer, David
AU - Glieder, Anton
AU - Kara, Selin
N1 - Publisher Copyright: © 2024 The Royal Society of Chemistry.
PY - 2024
Y1 - 2024
N2 - In the context of empowering biocatalysis, an easy-to-develop, reproducible, and easy-to-scale biocatalytic system under continuous flow is demonstrated. 3D printing technology is used as the reactor manufacturing method, yielding identical and low-cost microfluidic chips that can be further modified to serve as a biocatalytic platform for simultaneous parameter screening. The model enzyme studied here is unspecific peroxygenase (UPO). UPOs are currently under intensive study, due to their distinct promiscuity in oxyfunctionalization chemistry. This is the first study demonstrating UPO's immobilization in a microfluidic concept. The developed method for surface functionalization of microfluidic reactors is based on polydopamine modification and was proven highly reproducible. UPO showed a TTN of 19 249 and a STY of 2.1 g L -1 h -1, under the specified conditions. The kinetic behavior of the system under flow conditions is reported. The system was also regenerated with a 51.4% recovered activity. Further utilization of microfluidic concepts is expected to unravel the full potential of UPOs for oxyfunctionalization reactions of particular interest. The proposed system is foreseen as a screening platform for different reaction conditions, reaction substrates, or enzyme mutants.
AB - In the context of empowering biocatalysis, an easy-to-develop, reproducible, and easy-to-scale biocatalytic system under continuous flow is demonstrated. 3D printing technology is used as the reactor manufacturing method, yielding identical and low-cost microfluidic chips that can be further modified to serve as a biocatalytic platform for simultaneous parameter screening. The model enzyme studied here is unspecific peroxygenase (UPO). UPOs are currently under intensive study, due to their distinct promiscuity in oxyfunctionalization chemistry. This is the first study demonstrating UPO's immobilization in a microfluidic concept. The developed method for surface functionalization of microfluidic reactors is based on polydopamine modification and was proven highly reproducible. UPO showed a TTN of 19 249 and a STY of 2.1 g L -1 h -1, under the specified conditions. The kinetic behavior of the system under flow conditions is reported. The system was also regenerated with a 51.4% recovered activity. Further utilization of microfluidic concepts is expected to unravel the full potential of UPOs for oxyfunctionalization reactions of particular interest. The proposed system is foreseen as a screening platform for different reaction conditions, reaction substrates, or enzyme mutants.
UR - http://www.scopus.com/inward/record.url?scp=85207467282&partnerID=8YFLogxK
U2 - 10.1039/d4cy00869c
DO - 10.1039/d4cy00869c
M3 - Article
VL - 14
SP - 6496
EP - 6502
JO - Catalysis science & technology
JF - Catalysis science & technology
SN - 2044-4753
IS - 22
ER -