Details
| Original language | English |
|---|---|
| Pages (from-to) | 3073-3079 |
| Number of pages | 7 |
| Journal | FEBS letters |
| Volume | 582 |
| Issue number | 20 |
| Publication status | Published - 3 Sept 2008 |
| Externally published | Yes |
Abstract
We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria.
Keywords
- Alternative NAD(P)H dehydrogenase, Chloroplast, Dual targeting, Green fluorescent protein, Mitochondria, Peroxisome
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biophysics
- Biochemistry, Genetics and Molecular Biology(all)
- Structural Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: FEBS letters, Vol. 582, No. 20, 03.09.2008, p. 3073-3079.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana
AU - Carrie, Chris
AU - Murcha, Monika W.
AU - Kuehn, Kristina
AU - Duncan, Owen
AU - Barthet, Michelle
AU - Smith, Penelope M.
AU - Eubel, Holger
AU - Meyer, Etienne
AU - Day, David A.
AU - Millar, A. Harvey
AU - Whelan, James
N1 - Funding information: This work was supported by an Australian Research Council Grant DP0664692, ARC Australian Postdoctoral Fellowships to M.W.M. and H.E., and an ARC Australian Professorial Fellowship to A.H.M.
PY - 2008/9/3
Y1 - 2008/9/3
N2 - We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria.
AB - We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria.
KW - Alternative NAD(P)H dehydrogenase
KW - Chloroplast
KW - Dual targeting
KW - Green fluorescent protein
KW - Mitochondria
KW - Peroxisome
UR - http://www.scopus.com/inward/record.url?scp=49649083497&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2008.07.061
DO - 10.1016/j.febslet.2008.07.061
M3 - Article
C2 - 18703057
AN - SCOPUS:49649083497
VL - 582
SP - 3073
EP - 3079
JO - FEBS letters
JF - FEBS letters
SN - 0014-5793
IS - 20
ER -