Details
| Original language | English |
|---|---|
| Pages (from-to) | 725-738 |
| Number of pages | 14 |
| Journal | Regenerative medicine |
| Volume | 8 |
| Issue number | 6 |
| Publication status | Published - 22 Oct 2013 |
| Externally published | Yes |
Abstract
Aim: In this study, the suitability of a mixture containing riboflavin (vitamin B2) and triethanolamine (TEOHA) as a novel biocompatible photoinitiator for two-photon polymerization (2PP) processing was investigated. Materials & methods: Polyethylene glycol diacrylate was crosslinked using Irgacure® 369, Irgacure 2959 or a riboflavin-TEOHA mixture; biocompatibility of the photopolymer extract solutions was subsequently assessed via endothelial cell proliferation assay, endothelial cell viability assay and single-cell gel electrophoresis (comet) assay. Use of a riboflavin-TEOHA mixture as a photoinitiator for 2PP processing of a tissue engineering scaffold and subsequent seeding of this scaffold with GM-7373 bovine aortic endothelial cells was also demonstrated. Results: The riboflavin-TEOHA mixture was found to produce much more biocompatible scaffolds than those produced with Irgacure 369 or Irgacure 2959. Conclusion: The results suggest that riboflavin is a promising component of photoinitiators for 2PP fabrication of tissue engineering scaffolds and other medically relevant structures (e.g., biomicroelectromechanical systems).
Keywords
- biocompatibility, polyethylene glycol diacrylate, riboflavin, tissue engineering scaffolds, triethanolamine, two-photon polymerization
ASJC Scopus subject areas
- Engineering(all)
- Biomedical Engineering
- Medicine(all)
- Embryology
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In: Regenerative medicine, Vol. 8, No. 6, 22.10.2013, p. 725-738.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Two-photon polymerization of polyethylene glycol diacrylate scaffolds with riboflavin and triethanolamine used as a water-soluble photoinitiator
AU - Nguyen, Alexander K.
AU - Gittard, Shaun D.
AU - Koroleva, Anastasia
AU - Schlie, Sabrina
AU - Gaidukeviciute, Arune
AU - Chichkov, Boris N.
AU - Narayan, Roger J.
PY - 2013/10/22
Y1 - 2013/10/22
N2 - Aim: In this study, the suitability of a mixture containing riboflavin (vitamin B2) and triethanolamine (TEOHA) as a novel biocompatible photoinitiator for two-photon polymerization (2PP) processing was investigated. Materials & methods: Polyethylene glycol diacrylate was crosslinked using Irgacure® 369, Irgacure 2959 or a riboflavin-TEOHA mixture; biocompatibility of the photopolymer extract solutions was subsequently assessed via endothelial cell proliferation assay, endothelial cell viability assay and single-cell gel electrophoresis (comet) assay. Use of a riboflavin-TEOHA mixture as a photoinitiator for 2PP processing of a tissue engineering scaffold and subsequent seeding of this scaffold with GM-7373 bovine aortic endothelial cells was also demonstrated. Results: The riboflavin-TEOHA mixture was found to produce much more biocompatible scaffolds than those produced with Irgacure 369 or Irgacure 2959. Conclusion: The results suggest that riboflavin is a promising component of photoinitiators for 2PP fabrication of tissue engineering scaffolds and other medically relevant structures (e.g., biomicroelectromechanical systems).
AB - Aim: In this study, the suitability of a mixture containing riboflavin (vitamin B2) and triethanolamine (TEOHA) as a novel biocompatible photoinitiator for two-photon polymerization (2PP) processing was investigated. Materials & methods: Polyethylene glycol diacrylate was crosslinked using Irgacure® 369, Irgacure 2959 or a riboflavin-TEOHA mixture; biocompatibility of the photopolymer extract solutions was subsequently assessed via endothelial cell proliferation assay, endothelial cell viability assay and single-cell gel electrophoresis (comet) assay. Use of a riboflavin-TEOHA mixture as a photoinitiator for 2PP processing of a tissue engineering scaffold and subsequent seeding of this scaffold with GM-7373 bovine aortic endothelial cells was also demonstrated. Results: The riboflavin-TEOHA mixture was found to produce much more biocompatible scaffolds than those produced with Irgacure 369 or Irgacure 2959. Conclusion: The results suggest that riboflavin is a promising component of photoinitiators for 2PP fabrication of tissue engineering scaffolds and other medically relevant structures (e.g., biomicroelectromechanical systems).
KW - biocompatibility
KW - polyethylene glycol diacrylate
KW - riboflavin
KW - tissue engineering scaffolds
KW - triethanolamine
KW - two-photon polymerization
UR - http://www.scopus.com/inward/record.url?scp=84886557445&partnerID=8YFLogxK
U2 - 10.2217/rme.13.60
DO - 10.2217/rme.13.60
M3 - Article
C2 - 24147528
AN - SCOPUS:84886557445
VL - 8
SP - 725
EP - 738
JO - Regenerative medicine
JF - Regenerative medicine
SN - 1746-0751
IS - 6
ER -