Details
| Original language | English |
|---|---|
| Title of host publication | Environmental Monitoring and Remediation Technologies II |
| Pages | 42-48 |
| Number of pages | 7 |
| Volume | 3853 |
| Publication status | Published - 21 Dec 1999 |
| Event | PHOTONICS EAST '99: 1999 Environmental Monitoring and Remediation Technologies II - Boston, MA, USA Duration: 19 Sept 1999 → 22 Sept 1999 |
Publication series
| Name | Proceedings of SPIE - The International Society for Optical Engineering |
|---|---|
| Publisher | SPIE |
| ISSN (Print) | 0277-786X |
Abstract
Monitoring and control of industrial or biotechnological processes invariably require reliable and fast analytical systems. Two-dimensional spectral fluorescence allows real time automatic measurements directly inside the process and provides a continuous stream of information compared to discrete information available from repeated sampling and offline analysis. Normally, chromatographic molasses desugarization processes are typically monitored with a combination of an online-refractometer, polarimeter and the measurement of density and conductivity. Additional information of the separation profile could be obtained by multiple sampling during the separation cycle followed by offline laboratory analysis. An optical sensor (BioView) allows online fluorescence measurements for a continuous monitoring directly at the outlet of the separation columns. It was the aim to predict the amino acid serine during the chromatographic cycle. Based on fluorescence, it was possible to monitor a fluorophor, which eluted a few minutes before the serine fraction during molasses desugarization. The application of fluorescence measurements for monitoring and control of chromatographic separation process may improve yield and purity of the separating fractions and can lower the costs for the next downstream processing of by-products.
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Physics and Astronomy(all)
- Condensed Matter Physics
- Computer Science(all)
- Computer Science Applications
- Mathematics(all)
- Applied Mathematics
- Engineering(all)
- Electrical and Electronic Engineering
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Environmental Monitoring and Remediation Technologies II. Vol. 3853 1999. p. 42-48 (Proceedings of SPIE - The International Society for Optical Engineering).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Two-dimensional fluorescence spectroscopy for online-monitoring of chromatographic separation of molasses
AU - Staerk, E.
AU - Harbeck, C.
AU - Faurie, R.
AU - Lindemann, C.
AU - Scheper, T.
PY - 1999/12/21
Y1 - 1999/12/21
N2 - Monitoring and control of industrial or biotechnological processes invariably require reliable and fast analytical systems. Two-dimensional spectral fluorescence allows real time automatic measurements directly inside the process and provides a continuous stream of information compared to discrete information available from repeated sampling and offline analysis. Normally, chromatographic molasses desugarization processes are typically monitored with a combination of an online-refractometer, polarimeter and the measurement of density and conductivity. Additional information of the separation profile could be obtained by multiple sampling during the separation cycle followed by offline laboratory analysis. An optical sensor (BioView) allows online fluorescence measurements for a continuous monitoring directly at the outlet of the separation columns. It was the aim to predict the amino acid serine during the chromatographic cycle. Based on fluorescence, it was possible to monitor a fluorophor, which eluted a few minutes before the serine fraction during molasses desugarization. The application of fluorescence measurements for monitoring and control of chromatographic separation process may improve yield and purity of the separating fractions and can lower the costs for the next downstream processing of by-products.
AB - Monitoring and control of industrial or biotechnological processes invariably require reliable and fast analytical systems. Two-dimensional spectral fluorescence allows real time automatic measurements directly inside the process and provides a continuous stream of information compared to discrete information available from repeated sampling and offline analysis. Normally, chromatographic molasses desugarization processes are typically monitored with a combination of an online-refractometer, polarimeter and the measurement of density and conductivity. Additional information of the separation profile could be obtained by multiple sampling during the separation cycle followed by offline laboratory analysis. An optical sensor (BioView) allows online fluorescence measurements for a continuous monitoring directly at the outlet of the separation columns. It was the aim to predict the amino acid serine during the chromatographic cycle. Based on fluorescence, it was possible to monitor a fluorophor, which eluted a few minutes before the serine fraction during molasses desugarization. The application of fluorescence measurements for monitoring and control of chromatographic separation process may improve yield and purity of the separating fractions and can lower the costs for the next downstream processing of by-products.
UR - http://www.scopus.com/inward/record.url?scp=0033324069&partnerID=8YFLogxK
U2 - 10.1117/12.372862
DO - 10.1117/12.372862
M3 - Conference contribution
AN - SCOPUS:0033324069
VL - 3853
T3 - Proceedings of SPIE - The International Society for Optical Engineering
SP - 42
EP - 48
BT - Environmental Monitoring and Remediation Technologies II
T2 - PHOTONICS EAST '99
Y2 - 19 September 1999 through 22 September 1999
ER -