Details
| Original language | English |
|---|---|
| Pages (from-to) | 410-417 |
| Number of pages | 8 |
| Journal | Journal of biotechnology |
| Volume | 121 |
| Issue number | 3 |
| Publication status | Published - 24 Aug 2005 |
Abstract
In industrial fed-batch cultivations it is often necessary to control substrate concentrations at a low level to prevent the production of overflow metabolites and thus optimize the biomass yield. A new method for on-line monitoring and fed-batch control based on fluorescence measurements has been developed. Via instantaneous in situ measurements and multivariate data analysis a chemometric model has been established, which enables the rapid detection of ethanol production at aerobic Saccharomyces cerevisiae fed-batch cultivations. The glucose feed rate is controlled by predicting the metabolic state directly from the fluorescence intensities. Thus, ethanol production could be avoided completely while increasing the biomass yield accordingly. The robust instrumentation is suitable for industrial applications.
Keywords
- Ethanol inhibition, Fed-batch, Fluorescence, On-line monitoring, S. cerevisiae
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 121, No. 3, 24.08.2005, p. 410-417.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Two-dimensional fluorescence spectroscopy: A novel approach for controlling fed-batch cultivations
AU - Hantelmann, K.
AU - Kollecker, M.
AU - Hüll, D.
AU - Hitzmann, B.
AU - Scheper, T.
PY - 2005/8/24
Y1 - 2005/8/24
N2 - In industrial fed-batch cultivations it is often necessary to control substrate concentrations at a low level to prevent the production of overflow metabolites and thus optimize the biomass yield. A new method for on-line monitoring and fed-batch control based on fluorescence measurements has been developed. Via instantaneous in situ measurements and multivariate data analysis a chemometric model has been established, which enables the rapid detection of ethanol production at aerobic Saccharomyces cerevisiae fed-batch cultivations. The glucose feed rate is controlled by predicting the metabolic state directly from the fluorescence intensities. Thus, ethanol production could be avoided completely while increasing the biomass yield accordingly. The robust instrumentation is suitable for industrial applications.
AB - In industrial fed-batch cultivations it is often necessary to control substrate concentrations at a low level to prevent the production of overflow metabolites and thus optimize the biomass yield. A new method for on-line monitoring and fed-batch control based on fluorescence measurements has been developed. Via instantaneous in situ measurements and multivariate data analysis a chemometric model has been established, which enables the rapid detection of ethanol production at aerobic Saccharomyces cerevisiae fed-batch cultivations. The glucose feed rate is controlled by predicting the metabolic state directly from the fluorescence intensities. Thus, ethanol production could be avoided completely while increasing the biomass yield accordingly. The robust instrumentation is suitable for industrial applications.
KW - Ethanol inhibition
KW - Fed-batch
KW - Fluorescence
KW - On-line monitoring
KW - S. cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=30944445000&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2005.07.016
DO - 10.1016/j.jbiotec.2005.07.016
M3 - Article
C2 - 16125265
AN - SCOPUS:30944445000
VL - 121
SP - 410
EP - 417
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 3
ER -