Details
| Original language | English |
|---|---|
| Pages (from-to) | 203-206 |
| Number of pages | 4 |
| Journal | Analytica chimica acta |
| Volume | 182 |
| Issue number | C |
| Publication status | Published - 1986 |
Abstract
Two fluorogenic substrates are described for the detection of Penicillin-G-acylase (EC 3.5.1.11) activity in coloured cell-containing media and inside single cells. A sensitive enzyme assay is based on the use of 7-phenylacetyl-4-alkylcoumarinylamides. Linear response is obtained in the range 20-300 μU m-1. After the cells have been made permeable, intracellular enzyme activity can be monitored. It is thus possible to label enzyme-containing cells and to distinguish them from enzyme-free cells by fluorescence microscopy or laser-flow cytometry.
ASJC Scopus subject areas
- Chemistry(all)
- Analytical Chemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Environmental Science(all)
- Environmental Chemistry
- Chemistry(all)
- Spectroscopy
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In: Analytica chimica acta, Vol. 182, No. C, 1986, p. 203-206.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Two new fluorogenic substrates for the detection of Penicillin-G-acylase activity
AU - Scheper, Th
AU - Weiss, M.
AU - Schügerl, K.
PY - 1986
Y1 - 1986
N2 - Two fluorogenic substrates are described for the detection of Penicillin-G-acylase (EC 3.5.1.11) activity in coloured cell-containing media and inside single cells. A sensitive enzyme assay is based on the use of 7-phenylacetyl-4-alkylcoumarinylamides. Linear response is obtained in the range 20-300 μU m-1. After the cells have been made permeable, intracellular enzyme activity can be monitored. It is thus possible to label enzyme-containing cells and to distinguish them from enzyme-free cells by fluorescence microscopy or laser-flow cytometry.
AB - Two fluorogenic substrates are described for the detection of Penicillin-G-acylase (EC 3.5.1.11) activity in coloured cell-containing media and inside single cells. A sensitive enzyme assay is based on the use of 7-phenylacetyl-4-alkylcoumarinylamides. Linear response is obtained in the range 20-300 μU m-1. After the cells have been made permeable, intracellular enzyme activity can be monitored. It is thus possible to label enzyme-containing cells and to distinguish them from enzyme-free cells by fluorescence microscopy or laser-flow cytometry.
UR - http://www.scopus.com/inward/record.url?scp=38249042851&partnerID=8YFLogxK
U2 - 10.1016/S0003-2670(00)82451-4
DO - 10.1016/S0003-2670(00)82451-4
M3 - Article
AN - SCOPUS:38249042851
VL - 182
SP - 203
EP - 206
JO - Analytica chimica acta
JF - Analytica chimica acta
SN - 0003-2670
IS - C
ER -