Details
| Original language | English |
|---|---|
| Article number | e43582 |
| Journal | ELIFE |
| Volume | 8 |
| Early online date | 22 Aug 2019 |
| Publication status | E-pub ahead of print - 22 Aug 2019 |
Abstract
Many eukaryotic proteins regulating phosphate (Pi) homeostasis contain SPX domains that are receptors for inositol pyrophosphates (PP-InsP), suggesting that PP-InsPs may regulate Pi homeostasis. Here we report that deletion of two diphosphoinositol pentakisphosphate kinases VIH1/2 impairs plant growth and leads to constitutive Pi starvation responses. Deletion of phosphate starvation response transcription factors partially rescues vih1 vih2 mutant phenotypes, placing diphosphoinositol pentakisphosphate kinases in plant Pi signal transduction cascades. VIH1/ 2 are bifunctional enzymes able to generate and break-down PP-InsPs. Mutations in the kinase active site lead to increased Pi levels and constitutive Pi starvation responses. ATP levels change significantly in different Pi growth conditions. ATP-Mg2+ concentrations shift the relative kinase and phosphatase activities of diphosphoinositol pentakisphosphate kinases in vitro. Pi inhibits the phosphatase activity of the enzyme. Thus, VIH1 and VIH2 relay changes in cellular ATP and Pi concentrations to changes in PP-InsP levels, allowing plants to maintain sufficient Pi levels.
ASJC Scopus subject areas
- Neuroscience(all)
- General Neuroscience
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
- Immunology and Microbiology(all)
- General Immunology and Microbiology
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In: ELIFE, Vol. 8, e43582, 22.08.2019.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Two bifunctional inositol pyrophosphate kinases/phosphatases control plant phosphate homeostasis
AU - Zhu, Jinsheng
AU - Lau, Kelvin
AU - Puschmann, Robert
AU - Harmel, Robert K.
AU - Zhang, Youjun
AU - Pries, Verena
AU - Gaugler, Philipp
AU - Broger, Larissa
AU - Dutta, Amit K.
AU - Jessen, Henning J.
AU - Schaaf, Gabriel
AU - Fernie, Alisdair R.
AU - Hothorn, Ludwig A.
AU - Fiedler, Dorothea
AU - Hothorn, Michael
N1 - Funding Information: This work was supported by European Research Council under the European Union’s Seventh Framework Programme (FP/2007–2013)/ERC Grant Agreement 310856 (to MH), by Swiss National Foundation Sinergia Grant CRSII5_170925 (to DF and MH), by grant SCHA 1274/4–1 from the Deutsche Forschungsgemeinschaft (to GS) and by an HHMI International Research Scholar Award (to MH). KL was supported by an EMBO long-term fellowship (ALTF-493–2015). RKH and RP were supported by the Leibniz Gemeinschaft (SAW-2017-FMP-1). YZ and ARF were supported by the Max-Planck Society and the European Union’s Horizon 2020 research and innovation program, project PlantaSYST. We thank D Couto, L Lorenzo-Orts, M Ried, J Savarin and Y Poirier for critically reading the manuscript.
PY - 2019/8/22
Y1 - 2019/8/22
N2 - Many eukaryotic proteins regulating phosphate (Pi) homeostasis contain SPX domains that are receptors for inositol pyrophosphates (PP-InsP), suggesting that PP-InsPs may regulate Pi homeostasis. Here we report that deletion of two diphosphoinositol pentakisphosphate kinases VIH1/2 impairs plant growth and leads to constitutive Pi starvation responses. Deletion of phosphate starvation response transcription factors partially rescues vih1 vih2 mutant phenotypes, placing diphosphoinositol pentakisphosphate kinases in plant Pi signal transduction cascades. VIH1/ 2 are bifunctional enzymes able to generate and break-down PP-InsPs. Mutations in the kinase active site lead to increased Pi levels and constitutive Pi starvation responses. ATP levels change significantly in different Pi growth conditions. ATP-Mg2+ concentrations shift the relative kinase and phosphatase activities of diphosphoinositol pentakisphosphate kinases in vitro. Pi inhibits the phosphatase activity of the enzyme. Thus, VIH1 and VIH2 relay changes in cellular ATP and Pi concentrations to changes in PP-InsP levels, allowing plants to maintain sufficient Pi levels.
AB - Many eukaryotic proteins regulating phosphate (Pi) homeostasis contain SPX domains that are receptors for inositol pyrophosphates (PP-InsP), suggesting that PP-InsPs may regulate Pi homeostasis. Here we report that deletion of two diphosphoinositol pentakisphosphate kinases VIH1/2 impairs plant growth and leads to constitutive Pi starvation responses. Deletion of phosphate starvation response transcription factors partially rescues vih1 vih2 mutant phenotypes, placing diphosphoinositol pentakisphosphate kinases in plant Pi signal transduction cascades. VIH1/ 2 are bifunctional enzymes able to generate and break-down PP-InsPs. Mutations in the kinase active site lead to increased Pi levels and constitutive Pi starvation responses. ATP levels change significantly in different Pi growth conditions. ATP-Mg2+ concentrations shift the relative kinase and phosphatase activities of diphosphoinositol pentakisphosphate kinases in vitro. Pi inhibits the phosphatase activity of the enzyme. Thus, VIH1 and VIH2 relay changes in cellular ATP and Pi concentrations to changes in PP-InsP levels, allowing plants to maintain sufficient Pi levels.
UR - http://www.scopus.com/inward/record.url?scp=85071788543&partnerID=8YFLogxK
U2 - 10.1101/467076
DO - 10.1101/467076
M3 - Article
C2 - 31436531
AN - SCOPUS:85071788543
VL - 8
JO - ELIFE
JF - ELIFE
SN - 2050-084X
M1 - e43582
ER -