Details
| Original language | English |
|---|---|
| Article number | 41187 |
| Journal | Scientific reports |
| Volume | 7 |
| Publication status | Published - 23 Jan 2017 |
Abstract
Bienertia sinuspersici is a terrestrial plant that performs C4 photosynthesis within individual cells through operating a carbon concentrating mechanism between different subcellular domains including two types of chloroplasts. It is currently unknown how differentiation of two highly specialized chloroplasts within the same cell occurs as no similar cases have been reported. Here we show that this differentiation in photosynthetic cells of B. sinuspersici is enabled by a transit peptide (TP) mediated selective protein targeting mechanism. Mutations in the TPs cause loss of selectivity but not general loss of chloroplast import, indicating the mechanism operates by specifically blocking protein accumulation in one chloroplast type. Hybrid studies indicate that this selectivity is transferable to transit peptides of plants which perform C4 by cooperative function of chloroplasts between two photosynthetic cells. Codon swap experiments as well as introducing an artificial bait mRNA show that RNA affects are not crucial for the sorting process. In summary, our analysis shows how the mechanism of subcellular targeting to form two types of chloroplast within the same cell can be achieved. This information is not only crucial for understanding single-cell C4 photosynthesis; it provides new insights in control of subcellular protein targeting in cell biology.
Keywords
- Amaranthaceae/metabolism, Chloroplasts/metabolism, Photosynthesis, Plant Leaves/metabolism, Plant Proteins/metabolism, Protein Transport, Protoplasts/metabolism, RNA, Messenger/metabolism
ASJC Scopus subject areas
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In: Scientific reports, Vol. 7, 41187, 23.01.2017.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Transit peptide elements mediate selective protein targeting to two different types of chloroplasts in the single-cell C4 species Bienertia sinuspersici
AU - Wimmer, Diana
AU - Bohnhorst, Philipp
AU - Shekhar, Vinay
AU - Hwang, Inhwan
AU - Offermann, Sascha
N1 - Publisher Copyright: © 2017 The Author(s).
PY - 2017/1/23
Y1 - 2017/1/23
N2 - Bienertia sinuspersici is a terrestrial plant that performs C4 photosynthesis within individual cells through operating a carbon concentrating mechanism between different subcellular domains including two types of chloroplasts. It is currently unknown how differentiation of two highly specialized chloroplasts within the same cell occurs as no similar cases have been reported. Here we show that this differentiation in photosynthetic cells of B. sinuspersici is enabled by a transit peptide (TP) mediated selective protein targeting mechanism. Mutations in the TPs cause loss of selectivity but not general loss of chloroplast import, indicating the mechanism operates by specifically blocking protein accumulation in one chloroplast type. Hybrid studies indicate that this selectivity is transferable to transit peptides of plants which perform C4 by cooperative function of chloroplasts between two photosynthetic cells. Codon swap experiments as well as introducing an artificial bait mRNA show that RNA affects are not crucial for the sorting process. In summary, our analysis shows how the mechanism of subcellular targeting to form two types of chloroplast within the same cell can be achieved. This information is not only crucial for understanding single-cell C4 photosynthesis; it provides new insights in control of subcellular protein targeting in cell biology.
AB - Bienertia sinuspersici is a terrestrial plant that performs C4 photosynthesis within individual cells through operating a carbon concentrating mechanism between different subcellular domains including two types of chloroplasts. It is currently unknown how differentiation of two highly specialized chloroplasts within the same cell occurs as no similar cases have been reported. Here we show that this differentiation in photosynthetic cells of B. sinuspersici is enabled by a transit peptide (TP) mediated selective protein targeting mechanism. Mutations in the TPs cause loss of selectivity but not general loss of chloroplast import, indicating the mechanism operates by specifically blocking protein accumulation in one chloroplast type. Hybrid studies indicate that this selectivity is transferable to transit peptides of plants which perform C4 by cooperative function of chloroplasts between two photosynthetic cells. Codon swap experiments as well as introducing an artificial bait mRNA show that RNA affects are not crucial for the sorting process. In summary, our analysis shows how the mechanism of subcellular targeting to form two types of chloroplast within the same cell can be achieved. This information is not only crucial for understanding single-cell C4 photosynthesis; it provides new insights in control of subcellular protein targeting in cell biology.
KW - Amaranthaceae/metabolism
KW - Chloroplasts/metabolism
KW - Photosynthesis
KW - Plant Leaves/metabolism
KW - Plant Proteins/metabolism
KW - Protein Transport
KW - Protoplasts/metabolism
KW - RNA, Messenger/metabolism
UR - http://www.scopus.com/inward/record.url?scp=85010430211&partnerID=8YFLogxK
U2 - 10.1038/srep41187
DO - 10.1038/srep41187
M3 - Article
C2 - 28112241
VL - 7
JO - Scientific reports
JF - Scientific reports
SN - 2045-2322
M1 - 41187
ER -