Details
| Original language | English |
|---|---|
| Pages (from-to) | 245-250 |
| Number of pages | 6 |
| Journal | Plant Cell, Tissue and Organ Culture |
| Volume | 102 |
| Issue number | 2 |
| Publication status | Published - Aug 2010 |
Abstract
The study of gene function in roses is hampered by the low efficiency of transformation systems and the long time span needed for the generation of transgenic plants. For some functional analyses, the transient expression of genes would be an efficient alternative. Based on current protocols for the transient expression of genes via the infiltration of Agrobacterium into plant tissues, we developed a transient expression system for rose petals. We used β-glucuronidase (GUS) as a marker gene to optimize several parameters with effects on GUS expression. The efficiency of expression was found to be dependent on the rose genotype, flower age, position of petals within a flower, Agrobacterium strain and temperature of co-cultivation. The highest GUS expression was recorded in petals of the middle whirls of half-bloomed flowers from cultivars of 'Pariser Charme' and 'Marvel'.
Keywords
- Agrobacterium mediated transient expression, Rosa, Rose petals
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Plant Cell, Tissue and Organ Culture, Vol. 102, No. 2, 08.2010, p. 245-250.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Transient gene expression in rose petals via Agrobacterium infiltration
AU - Yasmin, Aneela
AU - Debener, Thomas
N1 - Funding Information: Acknowledgments The first author is thankful to Deutscher Aka-demischer Austausch Dienst (DAAD), Higher Education Commission of Pakistan (HEC) and Sindh Agriculture University, Tandojam for the award of scholarship and study leave.
PY - 2010/8
Y1 - 2010/8
N2 - The study of gene function in roses is hampered by the low efficiency of transformation systems and the long time span needed for the generation of transgenic plants. For some functional analyses, the transient expression of genes would be an efficient alternative. Based on current protocols for the transient expression of genes via the infiltration of Agrobacterium into plant tissues, we developed a transient expression system for rose petals. We used β-glucuronidase (GUS) as a marker gene to optimize several parameters with effects on GUS expression. The efficiency of expression was found to be dependent on the rose genotype, flower age, position of petals within a flower, Agrobacterium strain and temperature of co-cultivation. The highest GUS expression was recorded in petals of the middle whirls of half-bloomed flowers from cultivars of 'Pariser Charme' and 'Marvel'.
AB - The study of gene function in roses is hampered by the low efficiency of transformation systems and the long time span needed for the generation of transgenic plants. For some functional analyses, the transient expression of genes would be an efficient alternative. Based on current protocols for the transient expression of genes via the infiltration of Agrobacterium into plant tissues, we developed a transient expression system for rose petals. We used β-glucuronidase (GUS) as a marker gene to optimize several parameters with effects on GUS expression. The efficiency of expression was found to be dependent on the rose genotype, flower age, position of petals within a flower, Agrobacterium strain and temperature of co-cultivation. The highest GUS expression was recorded in petals of the middle whirls of half-bloomed flowers from cultivars of 'Pariser Charme' and 'Marvel'.
KW - Agrobacterium mediated transient expression
KW - Rosa
KW - Rose petals
UR - http://www.scopus.com/inward/record.url?scp=77953976928&partnerID=8YFLogxK
U2 - 10.1007/s11240-010-9728-2
DO - 10.1007/s11240-010-9728-2
M3 - Article
AN - SCOPUS:77953976928
VL - 102
SP - 245
EP - 250
JO - Plant Cell, Tissue and Organ Culture
JF - Plant Cell, Tissue and Organ Culture
SN - 0167-6857
IS - 2
ER -