Details
| Original language | English |
|---|---|
| Pages (from-to) | 183-193 |
| Number of pages | 11 |
| Journal | Journal of biotechnology |
| Volume | 33 |
| Issue number | 2 |
| Publication status | Published - 31 Mar 1994 |
Abstract
NADH-dependent culture fluorescence is increasingly used for the characterization of the biological state of the cells, when their environment changes. For better interpretation of the signal variations time resolved measurements are necessary. Saccharomyces cerevisiae H620 strain was cultivated on synthetic medium in continuous operation and time resolved response to variation of the dissolved oxygen and glucose concentrations were monitored by measuring the concentrations of cell mass, glucose, ethanol, acetaldehyde, glycogen, trehalose, mannan, lipids, dissolved oxygen, O2 and CO2 in off-gas as well as the NADH-dependent culture fluorescence. The time resolved ethanol concentration and fluorescence intensity changes under different cultivation conditions are used to discuss the cell responses to their environmental change.
Keywords
- Cell response, Continuous cultivation, Saccharomyces cerevisiae
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Journal of biotechnology, Vol. 33, No. 2, 31.03.1994, p. 183-193.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Transient behaviour of continuously cultivated Baker's yeast during enforced variations of dissolved oxygen and glucose concentrations
AU - Abel, C.
AU - Linz, F.
AU - Scheper, T.
AU - Schügerl, K.
PY - 1994/3/31
Y1 - 1994/3/31
N2 - NADH-dependent culture fluorescence is increasingly used for the characterization of the biological state of the cells, when their environment changes. For better interpretation of the signal variations time resolved measurements are necessary. Saccharomyces cerevisiae H620 strain was cultivated on synthetic medium in continuous operation and time resolved response to variation of the dissolved oxygen and glucose concentrations were monitored by measuring the concentrations of cell mass, glucose, ethanol, acetaldehyde, glycogen, trehalose, mannan, lipids, dissolved oxygen, O2 and CO2 in off-gas as well as the NADH-dependent culture fluorescence. The time resolved ethanol concentration and fluorescence intensity changes under different cultivation conditions are used to discuss the cell responses to their environmental change.
AB - NADH-dependent culture fluorescence is increasingly used for the characterization of the biological state of the cells, when their environment changes. For better interpretation of the signal variations time resolved measurements are necessary. Saccharomyces cerevisiae H620 strain was cultivated on synthetic medium in continuous operation and time resolved response to variation of the dissolved oxygen and glucose concentrations were monitored by measuring the concentrations of cell mass, glucose, ethanol, acetaldehyde, glycogen, trehalose, mannan, lipids, dissolved oxygen, O2 and CO2 in off-gas as well as the NADH-dependent culture fluorescence. The time resolved ethanol concentration and fluorescence intensity changes under different cultivation conditions are used to discuss the cell responses to their environmental change.
KW - Cell response
KW - Continuous cultivation
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=0028346162&partnerID=8YFLogxK
U2 - 10.1016/0168-1656(94)90110-4
DO - 10.1016/0168-1656(94)90110-4
M3 - Article
AN - SCOPUS:0028346162
VL - 33
SP - 183
EP - 193
JO - Journal of biotechnology
JF - Journal of biotechnology
SN - 0168-1656
IS - 2
ER -