Details
| Original language | English |
|---|---|
| Title of host publication | Acta Horticulturae |
| Publisher | International Society for Horticultural Science |
| Pages | 103-108 |
| Number of pages | 6 |
| ISBN (print) | 9789066056923 |
| Publication status | Published - 2009 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 847 |
| ISSN (Print) | 0567-7572 |
Abstract
Kalanchoe blossfeldiana leaf explants were infected and transformed with Agrobacterium tumefaciens harbouring the plasmid pBEO210. This construct contains the mutated ethylene receptor gene etr1-1 from Arabidopsis thaliana under the control of the flower-specific fbp1-promoter from Petunia. Flowers of eight putative transgenic T0 plants exhibiting a significant NPTII protein content showed lower ethylene sensitivity than control plants after ethylene exposure. One of the transgenic lines (line 324) showed altered vegetative and flowering architecture and infertility. The T0 line with lowest ethylene sensitivity (line 300) was analyzed for expression and copy-number of the etr1-1 gene and crossed with the ethylene sensitive cultivar 'Celine' to create progeny. The etr1-1 gene was found to be expressed in petals and stamens but not in carpels, sepals and leaves, confirming that the fbp1 promoter induces etr1-1 expression in a flower-specific manner. Southern blotting, segregation data from the crossing, and PCR analysis of progeny plants revealed two unlinked T-DNA copies in line 300, which are segregated in Mendelian fashion to T1 plants. T1 plants with only one T-DNA copy are as ethylene resistant as the T0 plants and may be useful in breeding to confer ethylene insensitivity to other Kalanchoe cultivars.
Keywords
- Etr1-1 expression, Flower longevity, Flower-specific promoter, Kalanchoe blossfeldiana, Progeny
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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Acta Horticulturae. International Society for Horticultural Science, 2009. p. 103-108 (Acta Horticulturae; Vol. 847).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Transformation of Kalanchoe blossfeldiana for ethylene insensitivity
AU - Sanikhani, M.
AU - Mibus, H.
AU - Stummann, B. M.
AU - Serek, M.
PY - 2009
Y1 - 2009
N2 - Kalanchoe blossfeldiana leaf explants were infected and transformed with Agrobacterium tumefaciens harbouring the plasmid pBEO210. This construct contains the mutated ethylene receptor gene etr1-1 from Arabidopsis thaliana under the control of the flower-specific fbp1-promoter from Petunia. Flowers of eight putative transgenic T0 plants exhibiting a significant NPTII protein content showed lower ethylene sensitivity than control plants after ethylene exposure. One of the transgenic lines (line 324) showed altered vegetative and flowering architecture and infertility. The T0 line with lowest ethylene sensitivity (line 300) was analyzed for expression and copy-number of the etr1-1 gene and crossed with the ethylene sensitive cultivar 'Celine' to create progeny. The etr1-1 gene was found to be expressed in petals and stamens but not in carpels, sepals and leaves, confirming that the fbp1 promoter induces etr1-1 expression in a flower-specific manner. Southern blotting, segregation data from the crossing, and PCR analysis of progeny plants revealed two unlinked T-DNA copies in line 300, which are segregated in Mendelian fashion to T1 plants. T1 plants with only one T-DNA copy are as ethylene resistant as the T0 plants and may be useful in breeding to confer ethylene insensitivity to other Kalanchoe cultivars.
AB - Kalanchoe blossfeldiana leaf explants were infected and transformed with Agrobacterium tumefaciens harbouring the plasmid pBEO210. This construct contains the mutated ethylene receptor gene etr1-1 from Arabidopsis thaliana under the control of the flower-specific fbp1-promoter from Petunia. Flowers of eight putative transgenic T0 plants exhibiting a significant NPTII protein content showed lower ethylene sensitivity than control plants after ethylene exposure. One of the transgenic lines (line 324) showed altered vegetative and flowering architecture and infertility. The T0 line with lowest ethylene sensitivity (line 300) was analyzed for expression and copy-number of the etr1-1 gene and crossed with the ethylene sensitive cultivar 'Celine' to create progeny. The etr1-1 gene was found to be expressed in petals and stamens but not in carpels, sepals and leaves, confirming that the fbp1 promoter induces etr1-1 expression in a flower-specific manner. Southern blotting, segregation data from the crossing, and PCR analysis of progeny plants revealed two unlinked T-DNA copies in line 300, which are segregated in Mendelian fashion to T1 plants. T1 plants with only one T-DNA copy are as ethylene resistant as the T0 plants and may be useful in breeding to confer ethylene insensitivity to other Kalanchoe cultivars.
KW - Etr1-1 expression
KW - Flower longevity
KW - Flower-specific promoter
KW - Kalanchoe blossfeldiana
KW - Progeny
UR - http://www.scopus.com/inward/record.url?scp=75649144443&partnerID=8YFLogxK
U2 - 10.17660/ActaHortic.2009.847.12
DO - 10.17660/ActaHortic.2009.847.12
M3 - Conference contribution
AN - SCOPUS:75649144443
SN - 9789066056923
T3 - Acta Horticulturae
SP - 103
EP - 108
BT - Acta Horticulturae
PB - International Society for Horticultural Science
ER -