Details
| Original language | English |
|---|---|
| Article number | 4556 |
| Journal | International Journal of Molecular Sciences |
| Volume | 22 |
| Issue number | 9 |
| Early online date | 27 Apr 2021 |
| Publication status | Published - 1 May 2021 |
Abstract
The development of multifunctional nanoscale systems that can mediate efficient tumor targeting, together with high cellular internalization, is crucial for the diagnosis of glioma. The combination of imaging agents into one platform provides dual imaging and allows further surface modification with targeting ligands for specific glioma detection. Herein, transferrin (Tf)-decorated niosomes with integrated magnetic iron oxide nanoparticles (MIONs) and quantum dots (QDs) were formulated (PEGNIO/QDs/MIONs/Tf) for efficient imaging of glioma, supported by magnetic and active targeting. Transmission electron microscopy confirmed the complete co-encapsulation of MIONs and QDs in the niosomes. Flow cytometry analysis demonstrated enhanced cellular uptake of the niosomal formulation by glioma cells. In vitro imaging studies showed that PEG-NIO/QDs/MIONs/Tf produces an obvious negative-contrast enhancement effect on glioma cells by magnetic resonance imaging (MRI) and also improved fluorescence intensity under fluorescence microscopy. This novel platform represents the first niosome-based system which combines magnetic nanoparticles and QDs, and has application potential in dual-targeted imaging of glioma.
Keywords
- Glioma imaging, Iron oxide nanoparticles, Multifunctional niosomes, Quantum dots
ASJC Scopus subject areas
- Chemical Engineering(all)
- Catalysis
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
- Chemistry(all)
- Spectroscopy
- Computer Science(all)
- Computer Science Applications
- Chemistry(all)
- Physical and Theoretical Chemistry
- Chemistry(all)
- Organic Chemistry
- Chemistry(all)
- Inorganic Chemistry
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In: International Journal of Molecular Sciences, Vol. 22, No. 9, 4556, 01.05.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Transferrin-decorated niosomes with integrated inp/zns quantum dots and magnetic iron oxide nanoparticles
T2 - Dual targeting and imaging of glioma
AU - Seleci, Didem Ag
AU - Maurer, Viktor
AU - Barlas, Firat Baris
AU - Porsiel, Julian Cedric
AU - Temel, Bilal
AU - Ceylan, Elcin
AU - Timur, Suna
AU - Stahl, Frank
AU - Scheper, Thomas
AU - Garnweitner, Georg
N1 - Funding Information: Funding: This research was partially funded by Niedersächsisches Ministerium für Wissenschaft und Kultur through the “Quantum-and Nano-Metrology (QUANOMET)” initiative (ZN3245) within the project NP-1. Moreover, we acknowledge support from the German Research Foundation and the Open Access Publication Funds of Technische Universität Braunschweig.
PY - 2021/5/1
Y1 - 2021/5/1
N2 - The development of multifunctional nanoscale systems that can mediate efficient tumor targeting, together with high cellular internalization, is crucial for the diagnosis of glioma. The combination of imaging agents into one platform provides dual imaging and allows further surface modification with targeting ligands for specific glioma detection. Herein, transferrin (Tf)-decorated niosomes with integrated magnetic iron oxide nanoparticles (MIONs) and quantum dots (QDs) were formulated (PEGNIO/QDs/MIONs/Tf) for efficient imaging of glioma, supported by magnetic and active targeting. Transmission electron microscopy confirmed the complete co-encapsulation of MIONs and QDs in the niosomes. Flow cytometry analysis demonstrated enhanced cellular uptake of the niosomal formulation by glioma cells. In vitro imaging studies showed that PEG-NIO/QDs/MIONs/Tf produces an obvious negative-contrast enhancement effect on glioma cells by magnetic resonance imaging (MRI) and also improved fluorescence intensity under fluorescence microscopy. This novel platform represents the first niosome-based system which combines magnetic nanoparticles and QDs, and has application potential in dual-targeted imaging of glioma.
AB - The development of multifunctional nanoscale systems that can mediate efficient tumor targeting, together with high cellular internalization, is crucial for the diagnosis of glioma. The combination of imaging agents into one platform provides dual imaging and allows further surface modification with targeting ligands for specific glioma detection. Herein, transferrin (Tf)-decorated niosomes with integrated magnetic iron oxide nanoparticles (MIONs) and quantum dots (QDs) were formulated (PEGNIO/QDs/MIONs/Tf) for efficient imaging of glioma, supported by magnetic and active targeting. Transmission electron microscopy confirmed the complete co-encapsulation of MIONs and QDs in the niosomes. Flow cytometry analysis demonstrated enhanced cellular uptake of the niosomal formulation by glioma cells. In vitro imaging studies showed that PEG-NIO/QDs/MIONs/Tf produces an obvious negative-contrast enhancement effect on glioma cells by magnetic resonance imaging (MRI) and also improved fluorescence intensity under fluorescence microscopy. This novel platform represents the first niosome-based system which combines magnetic nanoparticles and QDs, and has application potential in dual-targeted imaging of glioma.
KW - Glioma imaging
KW - Iron oxide nanoparticles
KW - Multifunctional niosomes
KW - Quantum dots
UR - http://www.scopus.com/inward/record.url?scp=85104753811&partnerID=8YFLogxK
U2 - 10.3390/ijms22094556
DO - 10.3390/ijms22094556
M3 - Article
C2 - 33925347
AN - SCOPUS:85104753811
VL - 22
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 9
M1 - 4556
ER -