Details
| Original language | English |
|---|---|
| Pages (from-to) | 935-939 |
| Number of pages | 5 |
| Journal | Liebigs Annales |
| Issue number | 6 |
| Publication status | Published - Jun 1996 |
Abstract
The active site analogue 2 of alkaline phosphatase was synthesized and its hydrolytic activity was tested. Efficient hydrolysis was observed at pH > 8 at various concentrations of the hydrolytic species. The cyclic phosphate 3 derived from transesterification could be identified by 31P-NMR spectroscopy. A comparison of the hydrolysis results with the species distribution curve revealed the ZnL-OH species as the active complex. The complex stability constant (log KZnL) of ligand 7 was determined to be 13.7 (± 0.5). K′ for the hydrolysis of the p-nitrophenyl phosphate ester of propylene glycol (1) with 2 (0.02 M, pH = 8.35) is 1.5 × 10-3 s-1 at 50°C.
Keywords
- Phosphate ester transesterification, pH-dependent, Synthetic ribonucleases, Zinc, Zinc complexes
ASJC Scopus subject areas
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In: Liebigs Annales, No. 6, 06.1996, p. 935-939.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Transesterification of phosphodiester by a zinc-containing cyclen derivative
T2 - Identification of the active species
AU - Kalesse, Markus
AU - Loos, Andrea
PY - 1996/6
Y1 - 1996/6
N2 - The active site analogue 2 of alkaline phosphatase was synthesized and its hydrolytic activity was tested. Efficient hydrolysis was observed at pH > 8 at various concentrations of the hydrolytic species. The cyclic phosphate 3 derived from transesterification could be identified by 31P-NMR spectroscopy. A comparison of the hydrolysis results with the species distribution curve revealed the ZnL-OH species as the active complex. The complex stability constant (log KZnL) of ligand 7 was determined to be 13.7 (± 0.5). K′ for the hydrolysis of the p-nitrophenyl phosphate ester of propylene glycol (1) with 2 (0.02 M, pH = 8.35) is 1.5 × 10-3 s-1 at 50°C.
AB - The active site analogue 2 of alkaline phosphatase was synthesized and its hydrolytic activity was tested. Efficient hydrolysis was observed at pH > 8 at various concentrations of the hydrolytic species. The cyclic phosphate 3 derived from transesterification could be identified by 31P-NMR spectroscopy. A comparison of the hydrolysis results with the species distribution curve revealed the ZnL-OH species as the active complex. The complex stability constant (log KZnL) of ligand 7 was determined to be 13.7 (± 0.5). K′ for the hydrolysis of the p-nitrophenyl phosphate ester of propylene glycol (1) with 2 (0.02 M, pH = 8.35) is 1.5 × 10-3 s-1 at 50°C.
KW - Phosphate ester transesterification, pH-dependent
KW - Synthetic ribonucleases
KW - Zinc
KW - Zinc complexes
UR - http://www.scopus.com/inward/record.url?scp=33748969009&partnerID=8YFLogxK
U2 - 10.1002/jlac.199619960611
DO - 10.1002/jlac.199619960611
M3 - Article
AN - SCOPUS:33748969009
SP - 935
EP - 939
JO - Liebigs Annales
JF - Liebigs Annales
SN - 0947-3440
IS - 6
ER -