Details
| Original language | English |
|---|---|
| Pages (from-to) | 45-52 |
| Number of pages | 8 |
| Journal | Current genetics |
| Volume | 37 |
| Issue number | 1 |
| Publication status | Published - Jan 2000 |
Abstract
The mustard chloroplast DNA region spanning the ycf3 gene and part of the psaAB operon was investigated. The ycf3 gene reveals two class-II introns that are removed during processing to give a mature 0.7-kb transcript, but no RNA editing seems to be involved. RNase protection and RT-PCR experiments suggest cotranscription of ycf3 with the downstream psaA gene, possibly from a NEP promoter upstream of ycf3, whereas distinct ycf3 and psaA transcripts are each initiated from PEP promoters. This situation is reminiscent of that for the trnK-psbA gene region. The implications for light-regulated versus light-independent expression of photosystem core-protein genes are discussed.
Keywords
- Amino Acid Sequence, Base Sequence, DNA, Chloroplast/genetics, Exons/genetics, Gene Expression Regulation, Plant, Genes, Plant/genetics, Introns/genetics, Light-Harvesting Protein Complexes, Membrane Proteins/genetics, Molecular Sequence Data, Mustard Plant/cytology, Operon/genetics, Photosynthetic Reaction Center Complex Proteins/chemistry, Photosystem I Protein Complex, Plant Proteins/genetics, Plants, Medicinal, Promoter Regions, Genetic/genetics, RNA Splicing/genetics, RNA, Chloroplast/analysis, RNA, Messenger/analysis, Sequence Alignment, Terminator Regions, Genetic/genetics, Transcription, Genetic/genetics
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In: Current genetics, Vol. 37, No. 1, 01.2000, p. 45-52.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Transcripts and sequence elements suggest differential promoter usage within the ycf3-psaAB gene cluster on mustard (Sinapis alba L.) chloroplast DNA
AU - Summer, H
AU - Pfannschmidt, T
AU - Link, G
N1 - Funding information: We thank Sacha Baginsky and Karsten Ogrzewalla for helpful discussion. This work was supported by the Deutsche Forschungsgemeinschaft and the Fonds der Chemischen Industrie, Germany.
PY - 2000/1
Y1 - 2000/1
N2 - The mustard chloroplast DNA region spanning the ycf3 gene and part of the psaAB operon was investigated. The ycf3 gene reveals two class-II introns that are removed during processing to give a mature 0.7-kb transcript, but no RNA editing seems to be involved. RNase protection and RT-PCR experiments suggest cotranscription of ycf3 with the downstream psaA gene, possibly from a NEP promoter upstream of ycf3, whereas distinct ycf3 and psaA transcripts are each initiated from PEP promoters. This situation is reminiscent of that for the trnK-psbA gene region. The implications for light-regulated versus light-independent expression of photosystem core-protein genes are discussed.
AB - The mustard chloroplast DNA region spanning the ycf3 gene and part of the psaAB operon was investigated. The ycf3 gene reveals two class-II introns that are removed during processing to give a mature 0.7-kb transcript, but no RNA editing seems to be involved. RNase protection and RT-PCR experiments suggest cotranscription of ycf3 with the downstream psaA gene, possibly from a NEP promoter upstream of ycf3, whereas distinct ycf3 and psaA transcripts are each initiated from PEP promoters. This situation is reminiscent of that for the trnK-psbA gene region. The implications for light-regulated versus light-independent expression of photosystem core-protein genes are discussed.
KW - Amino Acid Sequence
KW - Base Sequence
KW - DNA, Chloroplast/genetics
KW - Exons/genetics
KW - Gene Expression Regulation, Plant
KW - Genes, Plant/genetics
KW - Introns/genetics
KW - Light-Harvesting Protein Complexes
KW - Membrane Proteins/genetics
KW - Molecular Sequence Data
KW - Mustard Plant/cytology
KW - Operon/genetics
KW - Photosynthetic Reaction Center Complex Proteins/chemistry
KW - Photosystem I Protein Complex
KW - Plant Proteins/genetics
KW - Plants, Medicinal
KW - Promoter Regions, Genetic/genetics
KW - RNA Splicing/genetics
KW - RNA, Chloroplast/analysis
KW - RNA, Messenger/analysis
KW - Sequence Alignment
KW - Terminator Regions, Genetic/genetics
KW - Transcription, Genetic/genetics
U2 - 10.1007/s002940050007
DO - 10.1007/s002940050007
M3 - Article
C2 - 10672444
VL - 37
SP - 45
EP - 52
JO - Current genetics
JF - Current genetics
SN - 0172-8083
IS - 1
ER -