Details
| Original language | English |
|---|---|
| Pages (from-to) | 111-125 |
| Number of pages | 15 |
| Journal | Scientia horticulturae |
| Volume | 222 |
| Early online date | 13 May 2017 |
| Publication status | Published - 19 Aug 2017 |
Abstract
Frequent replanting causes biotic stress due to an altered soil and rhizosphere biome and results in apple replant disease (ARD). The disease is expressed by diminished growth and negatively affects fruit yield and quality. Recent studies aiming at understanding ARD on a molecular level showed that ARD affected plants suffer from oxidative stress. Genes involved in secondary metabolism reactions play an important role in the molecular ARD response of roots. Although the root system has to endure the biotic stress attack in the first place, severe symptoms of ARD can be visualized on aboveground plant parts. The objective of this study was to examine the transcriptomic response of leaves representing the metabolically active aboveground parts of ARD challenged apple plants and to compare results with existing data for roots to generate a more complete picture of ARD affected molecular reactions. For this, biotic stress response genes induced by ARD in roots were studied in RT-qPCR analyses using leaves of ARD sensitive ‘M26’ plants grown in two ARD soils, also in a time-dependent approach. Furthermore, an RNA sequencing approach employing MACE (massive analysis of cDNA ends) for transcriptome profiling was performed in order to identify further leaf specific candidate genes. RT-qPCR analyses did not reveal major differences in root candidate gene expression, but MACE indicated the upregulation of common biotic stress response genes. However, potential systemic oxidative stress occurred and ‘M26’ plants did not develop an effective defense response to ARD.
Keywords
- Apple replant disease, Biotic stress response, Gene expression, MACE (massive analysis of cDNA ends), Malus domestica, Oxidative stress, Quantitative RT-PCR, RNA-Seq
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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In: Scientia horticulturae, Vol. 222, 19.08.2017, p. 111-125.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Transcriptome profiling in leaves representing aboveground parts of apple replant disease affected Malus domestica ‘M26’ plants
AU - Weiß, Stefan
AU - Winkelmann, Traud
N1 - Funding Information: This work was funded by the “Deutsche Forschungsgemeinschaft” (DFG, grant number: GRK 1798/1) and it was part of the GRK1798 “Signaling at the Plant-Soil Interface”. Publisher Copyright: © 2017 Elsevier B.V. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2017/8/19
Y1 - 2017/8/19
N2 - Frequent replanting causes biotic stress due to an altered soil and rhizosphere biome and results in apple replant disease (ARD). The disease is expressed by diminished growth and negatively affects fruit yield and quality. Recent studies aiming at understanding ARD on a molecular level showed that ARD affected plants suffer from oxidative stress. Genes involved in secondary metabolism reactions play an important role in the molecular ARD response of roots. Although the root system has to endure the biotic stress attack in the first place, severe symptoms of ARD can be visualized on aboveground plant parts. The objective of this study was to examine the transcriptomic response of leaves representing the metabolically active aboveground parts of ARD challenged apple plants and to compare results with existing data for roots to generate a more complete picture of ARD affected molecular reactions. For this, biotic stress response genes induced by ARD in roots were studied in RT-qPCR analyses using leaves of ARD sensitive ‘M26’ plants grown in two ARD soils, also in a time-dependent approach. Furthermore, an RNA sequencing approach employing MACE (massive analysis of cDNA ends) for transcriptome profiling was performed in order to identify further leaf specific candidate genes. RT-qPCR analyses did not reveal major differences in root candidate gene expression, but MACE indicated the upregulation of common biotic stress response genes. However, potential systemic oxidative stress occurred and ‘M26’ plants did not develop an effective defense response to ARD.
AB - Frequent replanting causes biotic stress due to an altered soil and rhizosphere biome and results in apple replant disease (ARD). The disease is expressed by diminished growth and negatively affects fruit yield and quality. Recent studies aiming at understanding ARD on a molecular level showed that ARD affected plants suffer from oxidative stress. Genes involved in secondary metabolism reactions play an important role in the molecular ARD response of roots. Although the root system has to endure the biotic stress attack in the first place, severe symptoms of ARD can be visualized on aboveground plant parts. The objective of this study was to examine the transcriptomic response of leaves representing the metabolically active aboveground parts of ARD challenged apple plants and to compare results with existing data for roots to generate a more complete picture of ARD affected molecular reactions. For this, biotic stress response genes induced by ARD in roots were studied in RT-qPCR analyses using leaves of ARD sensitive ‘M26’ plants grown in two ARD soils, also in a time-dependent approach. Furthermore, an RNA sequencing approach employing MACE (massive analysis of cDNA ends) for transcriptome profiling was performed in order to identify further leaf specific candidate genes. RT-qPCR analyses did not reveal major differences in root candidate gene expression, but MACE indicated the upregulation of common biotic stress response genes. However, potential systemic oxidative stress occurred and ‘M26’ plants did not develop an effective defense response to ARD.
KW - Apple replant disease
KW - Biotic stress response
KW - Gene expression
KW - MACE (massive analysis of cDNA ends)
KW - Malus domestica
KW - Oxidative stress
KW - Quantitative RT-PCR
KW - RNA-Seq
UR - http://www.scopus.com/inward/record.url?scp=85044727201&partnerID=8YFLogxK
U2 - 10.1016/j.scienta.2017.05.012
DO - 10.1016/j.scienta.2017.05.012
M3 - Article
AN - SCOPUS:85044727201
VL - 222
SP - 111
EP - 125
JO - Scientia horticulturae
JF - Scientia horticulturae
SN - 0304-4238
ER -