Details
| Original language | English |
|---|---|
| Pages (from-to) | 22-30 |
| Number of pages | 9 |
| Journal | Current opinion in biotechnology |
| Volume | 24 |
| Issue number | 1 |
| Publication status | Published - Feb 2013 |
Abstract
Up to date research in biology, biotechnology, and medicine requires fast genome and transcriptome analysis technologies for the investigation of cellular state, physiology, and activity. Here, microarray technology and next generation sequencing of transcripts (RNA-Seq) are state of the art. Since microarray technology is limited towards the amount of RNA, the quantification of transcript levels and the sequence information, RNA-Seq provides nearly unlimited possibilities in modern bioanalysis. This chapter presents a detailed description of next-generation sequencing (NGS), describes the impact of this technology on transcriptome analysis and explains its possibilities to explore the modern RNA world.
Keywords
- DNA/genetics, Gene Expression Profiling/methods, Genome/genetics, Humans, Microarray Analysis, RNA/analysis, Sequence Analysis, RNA/methods, Transcriptome/genetics
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In: Current opinion in biotechnology, Vol. 24, No. 1, 02.2013, p. 22-30.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Transcriptome analysis using next-generation sequencing
AU - Mutz, Kai-Oliver
AU - Heilkenbrinker, Alexandra
AU - Lönne, Maren
AU - Walter, Johanna-Gabriela
AU - Stahl, Frank
N1 - Copyright © 2012 Elsevier Ltd. All rights reserved.
PY - 2013/2
Y1 - 2013/2
N2 - Up to date research in biology, biotechnology, and medicine requires fast genome and transcriptome analysis technologies for the investigation of cellular state, physiology, and activity. Here, microarray technology and next generation sequencing of transcripts (RNA-Seq) are state of the art. Since microarray technology is limited towards the amount of RNA, the quantification of transcript levels and the sequence information, RNA-Seq provides nearly unlimited possibilities in modern bioanalysis. This chapter presents a detailed description of next-generation sequencing (NGS), describes the impact of this technology on transcriptome analysis and explains its possibilities to explore the modern RNA world.
AB - Up to date research in biology, biotechnology, and medicine requires fast genome and transcriptome analysis technologies for the investigation of cellular state, physiology, and activity. Here, microarray technology and next generation sequencing of transcripts (RNA-Seq) are state of the art. Since microarray technology is limited towards the amount of RNA, the quantification of transcript levels and the sequence information, RNA-Seq provides nearly unlimited possibilities in modern bioanalysis. This chapter presents a detailed description of next-generation sequencing (NGS), describes the impact of this technology on transcriptome analysis and explains its possibilities to explore the modern RNA world.
KW - DNA/genetics
KW - Gene Expression Profiling/methods
KW - Genome/genetics
KW - Humans
KW - Microarray Analysis
KW - RNA/analysis
KW - Sequence Analysis, RNA/methods
KW - Transcriptome/genetics
U2 - 10.1016/j.copbio.2012.09.004
DO - 10.1016/j.copbio.2012.09.004
M3 - Review article
C2 - 23020966
VL - 24
SP - 22
EP - 30
JO - Current opinion in biotechnology
JF - Current opinion in biotechnology
SN - 0958-1669
IS - 1
ER -