Details
| Original language | English |
|---|---|
| Article number | 162 |
| Journal | Frontiers in Plant Science |
| Volume | 10 |
| Early online date | 25 Feb 2019 |
| Publication status | Published - Feb 2019 |
Abstract
Rice-pathogenic Xanthomonas oryzae bacteria cause severe harvest loss and challenge a stable food supply. The pathogen virulence relies strongly on bacterial TALE (transcription activator-like effector) proteins that function as transcriptional activators inside the plant cell. To understand the plant targets of TALEs, we determined the genome sequences of the Indian X. oryzae pv. oryzae (Xoo) type strain ICMP 3125 T and the strain PXO142 from the Philippines. Their complete TALE repertoire was analyzed and genome-wide TALE targets in rice were characterized. Integrating computational target predictions and rice transcriptomics data, we were able to verify 12 specifically induced target rice genes. The TALEs of the Xoo strains were reconstructed and expressed in a TALE-free Xoo strain to attribute specific induced genes to individual TALEs. Using reporter assays, we could show that individual TALEs act directly on their target promoters. In particular, we show that TALE classes assigned by AnnoTALE reflect common target genes, and that TALE classes of Xoo and the related pathogen X. oryzae pv. oryzicola share more common target genes than previously believed. Taken together, we establish a detailed picture of TALE-induced plant processes that significantly expands our understanding of X. oryzae virulence strategies and will facilitate the development of novel resistances to overcome this important rice disease.
Keywords
- Genome, Plant pathogen, Rice, Salicylic acid, TALE, Type III effector, Virulence, Xanthomonas oryzae
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Frontiers in Plant Science, Vol. 10, 162, 02.2019.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Transcriptional reprogramming of rice cells by Xanthomonas oryzae TALEs
AU - Mücke, Stefanie
AU - Reschke, Maik
AU - Erkes, Annett
AU - Schwietzer, Claudia Alice
AU - Becker, Sebastian
AU - Streubel, Jana
AU - Morgan, Richard D.
AU - Wilson, Geoffrey G.
AU - Grau, Jan
AU - Boch, Jens
N1 - Funding information: This work was supported by grants from the Deutsche Forschungsgemeinschaft (BO 1496/8-1 to JB and GR 4587/1-1 to JG), by the COST actions FA1208 “SUSTAIN” and CA16107 “EuroXanth,” and from the European Regional Development Fund of the European Commission. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The publication of this article was funded by the Open Access Fund of the Leibniz Universität Hannover.
PY - 2019/2
Y1 - 2019/2
N2 - Rice-pathogenic Xanthomonas oryzae bacteria cause severe harvest loss and challenge a stable food supply. The pathogen virulence relies strongly on bacterial TALE (transcription activator-like effector) proteins that function as transcriptional activators inside the plant cell. To understand the plant targets of TALEs, we determined the genome sequences of the Indian X. oryzae pv. oryzae (Xoo) type strain ICMP 3125 T and the strain PXO142 from the Philippines. Their complete TALE repertoire was analyzed and genome-wide TALE targets in rice were characterized. Integrating computational target predictions and rice transcriptomics data, we were able to verify 12 specifically induced target rice genes. The TALEs of the Xoo strains were reconstructed and expressed in a TALE-free Xoo strain to attribute specific induced genes to individual TALEs. Using reporter assays, we could show that individual TALEs act directly on their target promoters. In particular, we show that TALE classes assigned by AnnoTALE reflect common target genes, and that TALE classes of Xoo and the related pathogen X. oryzae pv. oryzicola share more common target genes than previously believed. Taken together, we establish a detailed picture of TALE-induced plant processes that significantly expands our understanding of X. oryzae virulence strategies and will facilitate the development of novel resistances to overcome this important rice disease.
AB - Rice-pathogenic Xanthomonas oryzae bacteria cause severe harvest loss and challenge a stable food supply. The pathogen virulence relies strongly on bacterial TALE (transcription activator-like effector) proteins that function as transcriptional activators inside the plant cell. To understand the plant targets of TALEs, we determined the genome sequences of the Indian X. oryzae pv. oryzae (Xoo) type strain ICMP 3125 T and the strain PXO142 from the Philippines. Their complete TALE repertoire was analyzed and genome-wide TALE targets in rice were characterized. Integrating computational target predictions and rice transcriptomics data, we were able to verify 12 specifically induced target rice genes. The TALEs of the Xoo strains were reconstructed and expressed in a TALE-free Xoo strain to attribute specific induced genes to individual TALEs. Using reporter assays, we could show that individual TALEs act directly on their target promoters. In particular, we show that TALE classes assigned by AnnoTALE reflect common target genes, and that TALE classes of Xoo and the related pathogen X. oryzae pv. oryzicola share more common target genes than previously believed. Taken together, we establish a detailed picture of TALE-induced plant processes that significantly expands our understanding of X. oryzae virulence strategies and will facilitate the development of novel resistances to overcome this important rice disease.
KW - Genome
KW - Plant pathogen
KW - Rice
KW - Salicylic acid
KW - TALE
KW - Type III effector
KW - Virulence
KW - Xanthomonas oryzae
UR - http://www.scopus.com/inward/record.url?scp=85064211412&partnerID=8YFLogxK
U2 - 10.3389/fpls.2019.00162
DO - 10.3389/fpls.2019.00162
M3 - Article
C2 - 30858855
AN - SCOPUS:85064211412
VL - 10
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 162
ER -