Details
| Original language | English |
|---|---|
| Article number | 104995 |
| Journal | Applied geochemistry |
| Volume | 130 |
| Early online date | 14 May 2021 |
| Publication status | Published - Jul 2021 |
Abstract
Laboratory studies on chemolithoautotrophic microbial denitrification coupled to pyrite oxidation at circumneutral pH yielded conflicting results. Some studies indicated that microbial oxidation of pyrite does occur, but several reports have shown that no microbial pyrite oxidation took place in experiments with pyrite as electron donor and nitrate as electron acceptor. We propose that inconsistent experimental and analytical protocols may cause substantial uncertainty in the interpretation of results from such laboratory studies, and may even produce artifacts. In this study, a comprehensive overview of possible pitfalls and artifacts in relation to geochemical and microbiological interferences is provided. Key interferences are impurities of reduced sulfur species associated with pyrite, interferences of nitrite and dissolved Fe(III) with quantitative spectrophotometric determination of Fe(II) and Fe(III) during acidic extractions, interference of oxygen, occurrence of residual iron and sulfur compounds in the reaction medium, and interference of cell-associated and stored sulfur. Therefore, we propose a series of experimental standard protocols to overcome these interferences in future studies on chemolithoautotrophic denitrification with pyrite.
Keywords
- Conflicting results, Geochemical interference, Iron oxidation, Iron sulfide minerals, Microbiological interference, Nitrate reduction
ASJC Scopus subject areas
- Environmental Science(all)
- Environmental Chemistry
- Environmental Science(all)
- Pollution
- Earth and Planetary Sciences(all)
- Geochemistry and Petrology
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In: Applied geochemistry, Vol. 130, 104995, 07.2021.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Towards a standardized protocol for studying chemolithoautotrophic denitrification with pyrite at circumneutral pH
AU - Yan, Ruiwen
AU - Kappler, Andreas
AU - Horn, Marcus A.
AU - Peiffer, Stefan
N1 - Funding Information: This work was supported by Fundamental Research Funds for the Central Universities (Grant No. 2019QD03 ).
PY - 2021/7
Y1 - 2021/7
N2 - Laboratory studies on chemolithoautotrophic microbial denitrification coupled to pyrite oxidation at circumneutral pH yielded conflicting results. Some studies indicated that microbial oxidation of pyrite does occur, but several reports have shown that no microbial pyrite oxidation took place in experiments with pyrite as electron donor and nitrate as electron acceptor. We propose that inconsistent experimental and analytical protocols may cause substantial uncertainty in the interpretation of results from such laboratory studies, and may even produce artifacts. In this study, a comprehensive overview of possible pitfalls and artifacts in relation to geochemical and microbiological interferences is provided. Key interferences are impurities of reduced sulfur species associated with pyrite, interferences of nitrite and dissolved Fe(III) with quantitative spectrophotometric determination of Fe(II) and Fe(III) during acidic extractions, interference of oxygen, occurrence of residual iron and sulfur compounds in the reaction medium, and interference of cell-associated and stored sulfur. Therefore, we propose a series of experimental standard protocols to overcome these interferences in future studies on chemolithoautotrophic denitrification with pyrite.
AB - Laboratory studies on chemolithoautotrophic microbial denitrification coupled to pyrite oxidation at circumneutral pH yielded conflicting results. Some studies indicated that microbial oxidation of pyrite does occur, but several reports have shown that no microbial pyrite oxidation took place in experiments with pyrite as electron donor and nitrate as electron acceptor. We propose that inconsistent experimental and analytical protocols may cause substantial uncertainty in the interpretation of results from such laboratory studies, and may even produce artifacts. In this study, a comprehensive overview of possible pitfalls and artifacts in relation to geochemical and microbiological interferences is provided. Key interferences are impurities of reduced sulfur species associated with pyrite, interferences of nitrite and dissolved Fe(III) with quantitative spectrophotometric determination of Fe(II) and Fe(III) during acidic extractions, interference of oxygen, occurrence of residual iron and sulfur compounds in the reaction medium, and interference of cell-associated and stored sulfur. Therefore, we propose a series of experimental standard protocols to overcome these interferences in future studies on chemolithoautotrophic denitrification with pyrite.
KW - Conflicting results
KW - Geochemical interference
KW - Iron oxidation
KW - Iron sulfide minerals
KW - Microbiological interference
KW - Nitrate reduction
UR - http://www.scopus.com/inward/record.url?scp=85106469010&partnerID=8YFLogxK
U2 - 10.1016/j.apgeochem.2021.104995
DO - 10.1016/j.apgeochem.2021.104995
M3 - Article
VL - 130
JO - Applied geochemistry
JF - Applied geochemistry
SN - 0883-2927
M1 - 104995
ER -