Details
| Original language | English |
|---|---|
| Title of host publication | XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010) |
| Subtitle of host publication | International Symposium on Micro and Macro Technologies for Plant Propagation and Breeding in Horticulture |
| Editors | A. Fabbri, E. Rugini |
| Pages | 15-24 |
| Number of pages | 10 |
| Publication status | Published - 31 Dec 2011 |
Publication series
| Name | Acta Horticulturae |
|---|---|
| Volume | 923 |
| ISSN (Print) | 0567-7572 |
Abstract
Somatic embryogenesis in Cyclamen persicum was first reported in 1984 and has potential applications for propagation and breeding of this economically important ornamental crop. This in vitro regeneration system can be used for vegetative propagation of parental lines of F1 hybrids and elite plants, production of artificial seeds, Agrobacterium tumefaciens-mediated genetic transformation, long-term cryopreservation, protoplast to plant regeneration and somatic hybridization. Somatic embryogenesis was shown to be a powerful propagation system for some C. persicum genotypes, but commercial application in large scale so far is hindered by several limitations, i.e., asynchronous development, malformations or secondary somatic embryogenesis. However, recent molecular approaches by transcriptomic and proteomic analyses were undertaken in order to better understand and control this in vitro regeneration system and to overcome these problems. Our studies aim at comparing somatic embryos to their zygotic counterparts regarding their proteomes. Protein separation by two dimensional isoelectric focusing - sodium do-decyl sulfate polyacrylamide gel electrophoresis led to a resolution of about 1000 protein spots per gel, of which the first 253 were identified by mass spectrometry. Most were found to be involved in glycolysis/gluconeogenesis and stress response pathways. A proteome reference map of zygotic embryos will be publicly released soon and may serve as a basis for further investigations and improvements of somatic embryogenesis.
Keywords
- Breeding support, Mass propagation, Ornamental plant, Proteomics, Zygotic embryogenesis
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Horticulture
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XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Micro and Macro Technologies for Plant Propagation and Breeding in Horticulture. ed. / A. Fabbri; E. Rugini. 2011. p. 15-24 (Acta Horticulturae; Vol. 923).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Towards a Better Understanding of Somatic Embryogenesis in Cyclamen persicum
AU - Winkelmann, T.
AU - Rode, C.
AU - Bartsch, M.
AU - Prange, A.
AU - Heintz, D.
AU - Van Dorsselaer, A.
AU - Braun, H. P.
PY - 2011/12/31
Y1 - 2011/12/31
N2 - Somatic embryogenesis in Cyclamen persicum was first reported in 1984 and has potential applications for propagation and breeding of this economically important ornamental crop. This in vitro regeneration system can be used for vegetative propagation of parental lines of F1 hybrids and elite plants, production of artificial seeds, Agrobacterium tumefaciens-mediated genetic transformation, long-term cryopreservation, protoplast to plant regeneration and somatic hybridization. Somatic embryogenesis was shown to be a powerful propagation system for some C. persicum genotypes, but commercial application in large scale so far is hindered by several limitations, i.e., asynchronous development, malformations or secondary somatic embryogenesis. However, recent molecular approaches by transcriptomic and proteomic analyses were undertaken in order to better understand and control this in vitro regeneration system and to overcome these problems. Our studies aim at comparing somatic embryos to their zygotic counterparts regarding their proteomes. Protein separation by two dimensional isoelectric focusing - sodium do-decyl sulfate polyacrylamide gel electrophoresis led to a resolution of about 1000 protein spots per gel, of which the first 253 were identified by mass spectrometry. Most were found to be involved in glycolysis/gluconeogenesis and stress response pathways. A proteome reference map of zygotic embryos will be publicly released soon and may serve as a basis for further investigations and improvements of somatic embryogenesis.
AB - Somatic embryogenesis in Cyclamen persicum was first reported in 1984 and has potential applications for propagation and breeding of this economically important ornamental crop. This in vitro regeneration system can be used for vegetative propagation of parental lines of F1 hybrids and elite plants, production of artificial seeds, Agrobacterium tumefaciens-mediated genetic transformation, long-term cryopreservation, protoplast to plant regeneration and somatic hybridization. Somatic embryogenesis was shown to be a powerful propagation system for some C. persicum genotypes, but commercial application in large scale so far is hindered by several limitations, i.e., asynchronous development, malformations or secondary somatic embryogenesis. However, recent molecular approaches by transcriptomic and proteomic analyses were undertaken in order to better understand and control this in vitro regeneration system and to overcome these problems. Our studies aim at comparing somatic embryos to their zygotic counterparts regarding their proteomes. Protein separation by two dimensional isoelectric focusing - sodium do-decyl sulfate polyacrylamide gel electrophoresis led to a resolution of about 1000 protein spots per gel, of which the first 253 were identified by mass spectrometry. Most were found to be involved in glycolysis/gluconeogenesis and stress response pathways. A proteome reference map of zygotic embryos will be publicly released soon and may serve as a basis for further investigations and improvements of somatic embryogenesis.
KW - Breeding support
KW - Mass propagation
KW - Ornamental plant
KW - Proteomics
KW - Zygotic embryogenesis
UR - http://www.scopus.com/inward/record.url?scp=84863674895&partnerID=8YFLogxK
U2 - 10.15488/11658
DO - 10.15488/11658
M3 - Conference contribution
AN - SCOPUS:84863674895
SN - 9789066055841
T3 - Acta Horticulturae
SP - 15
EP - 24
BT - XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010)
A2 - Fabbri, A.
A2 - Rugini, E.
ER -