Details
| Original language | English |
|---|---|
| Article number | e0205411 |
| Journal | PLOS ONE |
| Volume | 13 |
| Issue number | 10 |
| Publication status | Published - 10 Oct 2018 |
Abstract
It is estimated that two million new dental implants are inserted worldwide each year. Innovative implant materials are developed in order to minimize the risk of peri-implant inflammations. The broad range of material testing is conducted using standard 2D, terminal, and invasive methods. The methods that have been applied are not sufficient to monitor the whole implant surface and temporal progress. Therefore, we built a 3D peri-implant model using a cylindrical implant colonized by human gingival fibroblasts. In order to monitor the cell response over time, a non-toxic LIVE/DEAD staining was established and applied to the new 3D model. Our LIVE/DEAD staining method in combination with the time resolved 3D visualization using Scanning Laser Optical Tomography (SLOT), allowed us to monitor the cell death path along the implant in the 3D peri-implant model. The differentiation of living and dead gingival fibroblasts in response to toxicity was effectively supported by the LIVE/ DEAD staining. Furthermore, it was possible to visualize the whole cell-colonized implant in 3D and up to 63 hours. This new methodology offers the opportunity to record the long-term cell response on external stress factors, along the dental implant and thus to evaluate the performance of novel materials/surfaces.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- General Biochemistry,Genetics and Molecular Biology
- Agricultural and Biological Sciences(all)
- General Agricultural and Biological Sciences
- General
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In: PLOS ONE, Vol. 13, No. 10, e0205411, 10.10.2018.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Time resolved 3D live-cell imaging on implants
AU - Ingendoh-Tsakmakidis, Alexandra
AU - Nolte, Lena
AU - Winkel, Andreas
AU - Meyer, Heiko
AU - Koroleva, Anastasia
AU - Shpichka, Anastasia
AU - Ripken, Tammo
AU - Heisterkamp, Alexander
AU - Stiesch, Meike
N1 - Funding Information: The BIOFABRICATION FOR NIFE Initiative is financially supported by the ministry of Lower Saxony and the VolkswagenStiftung (both BIOFABRICATION FOR NIFE: VWZN2860). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The current study was conducted within the interdisciplinary research consortium BIOFABRICATION for NIFE. We would like to thank Henning Hartwig for contributing to the protocol of in vitro material colonization with human cells. Thanks go to Merna Haddad for her support during preliminary testing. Furthermore, we would like to thank the program developers of the open source software ImageJ (rsbweb.nih.gov/ij/).
PY - 2018/10/10
Y1 - 2018/10/10
N2 - It is estimated that two million new dental implants are inserted worldwide each year. Innovative implant materials are developed in order to minimize the risk of peri-implant inflammations. The broad range of material testing is conducted using standard 2D, terminal, and invasive methods. The methods that have been applied are not sufficient to monitor the whole implant surface and temporal progress. Therefore, we built a 3D peri-implant model using a cylindrical implant colonized by human gingival fibroblasts. In order to monitor the cell response over time, a non-toxic LIVE/DEAD staining was established and applied to the new 3D model. Our LIVE/DEAD staining method in combination with the time resolved 3D visualization using Scanning Laser Optical Tomography (SLOT), allowed us to monitor the cell death path along the implant in the 3D peri-implant model. The differentiation of living and dead gingival fibroblasts in response to toxicity was effectively supported by the LIVE/ DEAD staining. Furthermore, it was possible to visualize the whole cell-colonized implant in 3D and up to 63 hours. This new methodology offers the opportunity to record the long-term cell response on external stress factors, along the dental implant and thus to evaluate the performance of novel materials/surfaces.
AB - It is estimated that two million new dental implants are inserted worldwide each year. Innovative implant materials are developed in order to minimize the risk of peri-implant inflammations. The broad range of material testing is conducted using standard 2D, terminal, and invasive methods. The methods that have been applied are not sufficient to monitor the whole implant surface and temporal progress. Therefore, we built a 3D peri-implant model using a cylindrical implant colonized by human gingival fibroblasts. In order to monitor the cell response over time, a non-toxic LIVE/DEAD staining was established and applied to the new 3D model. Our LIVE/DEAD staining method in combination with the time resolved 3D visualization using Scanning Laser Optical Tomography (SLOT), allowed us to monitor the cell death path along the implant in the 3D peri-implant model. The differentiation of living and dead gingival fibroblasts in response to toxicity was effectively supported by the LIVE/ DEAD staining. Furthermore, it was possible to visualize the whole cell-colonized implant in 3D and up to 63 hours. This new methodology offers the opportunity to record the long-term cell response on external stress factors, along the dental implant and thus to evaluate the performance of novel materials/surfaces.
UR - http://www.scopus.com/inward/record.url?scp=85054777621&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0205411
DO - 10.1371/journal.pone.0205411
M3 - Article
C2 - 30304039
AN - SCOPUS:85054777621
VL - 13
JO - PLOS ONE
JF - PLOS ONE
SN - 1932-6203
IS - 10
M1 - e0205411
ER -