Details
| Original language | English |
|---|---|
| Pages (from-to) | 126 - 161 |
| Number of pages | 36 |
| Journal | Tissue Engineering - Part B: Reviews |
| Volume | 31 |
| Issue number | 2 |
| Early online date | 5 Feb 2025 |
| Publication status | Published - 14 Apr 2025 |
Abstract
Vascular surgery is facing a critical demand for novel vascular grafts that are biocompatible and thromboresistant. This urgency is particularly applicable to bypass operations involving small caliber vessels. In the realm of tissue engineering, the development of fully vascularized organs is promising as a solution to organ shortage for transplantation. To achieve this, it is essential to (re)construct a biocompatible and nonthrombogenic vascular network within these organs. In this systematic review, we identify, classify, and discuss basic principles and methods used to perform in vitro/ex vivo dynamic thrombogenicity testing of perfusable tissue-engineered organs and tissues. We conducted a preregistered systematic review of studies published in the last 23 years according to PRISMA-P Guidelines. This comprised a systematic data extraction, in-depth analysis, and risk of bias assessment of 116 included studies. We identified shaking (n = 28), flow loop (n = 17), ex vivo (arteriovenous shunt, n = 33), and dynamic in vitro models (n = 38) as the main approaches for thrombogenicity assessment. This comprehensive review reveals a prevalent lack of standardization and provides a valuable guide in the design of standardized experimental setups.
Keywords
- perfusion, thrombogenicity, tissue engineering, whole blood
ASJC Scopus subject areas
- Chemical Engineering(all)
- Bioengineering
- Materials Science(all)
- Biomaterials
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Engineering(all)
- Biomedical Engineering
Cite this
- Standard
- Harvard
- Apa
- Vancouver
- BibTeX
- RIS
In: Tissue Engineering - Part B: Reviews, Vol. 31, No. 2, 14.04.2025, p. 126 - 161.
Research output: Contribution to journal › Review article › Research › peer review
}
TY - JOUR
T1 - Thrombogenicity Assessment of Perfusable Tissue-Engineered Constructs
T2 - A Systematic Review
AU - Haderer, Luna
AU - Zhou, Yijun
AU - Tang, Peter
AU - Daneshgar, Assal
AU - Globke, Brigitta
AU - Krenzien, Felix
AU - Reutzel-Selke, Anja
AU - Weinhart, Marie
AU - Pratschke, Johann
AU - Sauer, Igor Maximillian
AU - Hillebrandt, Karl Herbert
AU - Keshi, Eriselda
N1 - Publisher Copyright: Copyright 2025, Mary Ann Liebert, Inc., publishers.
PY - 2025/4/14
Y1 - 2025/4/14
N2 - Vascular surgery is facing a critical demand for novel vascular grafts that are biocompatible and thromboresistant. This urgency is particularly applicable to bypass operations involving small caliber vessels. In the realm of tissue engineering, the development of fully vascularized organs is promising as a solution to organ shortage for transplantation. To achieve this, it is essential to (re)construct a biocompatible and nonthrombogenic vascular network within these organs. In this systematic review, we identify, classify, and discuss basic principles and methods used to perform in vitro/ex vivo dynamic thrombogenicity testing of perfusable tissue-engineered organs and tissues. We conducted a preregistered systematic review of studies published in the last 23 years according to PRISMA-P Guidelines. This comprised a systematic data extraction, in-depth analysis, and risk of bias assessment of 116 included studies. We identified shaking (n = 28), flow loop (n = 17), ex vivo (arteriovenous shunt, n = 33), and dynamic in vitro models (n = 38) as the main approaches for thrombogenicity assessment. This comprehensive review reveals a prevalent lack of standardization and provides a valuable guide in the design of standardized experimental setups.
AB - Vascular surgery is facing a critical demand for novel vascular grafts that are biocompatible and thromboresistant. This urgency is particularly applicable to bypass operations involving small caliber vessels. In the realm of tissue engineering, the development of fully vascularized organs is promising as a solution to organ shortage for transplantation. To achieve this, it is essential to (re)construct a biocompatible and nonthrombogenic vascular network within these organs. In this systematic review, we identify, classify, and discuss basic principles and methods used to perform in vitro/ex vivo dynamic thrombogenicity testing of perfusable tissue-engineered organs and tissues. We conducted a preregistered systematic review of studies published in the last 23 years according to PRISMA-P Guidelines. This comprised a systematic data extraction, in-depth analysis, and risk of bias assessment of 116 included studies. We identified shaking (n = 28), flow loop (n = 17), ex vivo (arteriovenous shunt, n = 33), and dynamic in vitro models (n = 38) as the main approaches for thrombogenicity assessment. This comprehensive review reveals a prevalent lack of standardization and provides a valuable guide in the design of standardized experimental setups.
KW - perfusion
KW - thrombogenicity
KW - tissue engineering
KW - whole blood
UR - http://www.scopus.com/inward/record.url?scp=85217020203&partnerID=8YFLogxK
U2 - 10.1089/ten.teb.2024.0078
DO - 10.1089/ten.teb.2024.0078
M3 - Review article
C2 - 39007511
AN - SCOPUS:85217020203
VL - 31
SP - 126
EP - 161
JO - Tissue Engineering - Part B: Reviews
JF - Tissue Engineering - Part B: Reviews
SN - 1937-3368
IS - 2
ER -