Three-dimensional shape estimation of BHK cell clusters from a still image based on shape from shading for in-situ microscopy

Research output: Chapter in book/report/conference proceedingConference contributionResearchpeer review

Authors

  • G. Martinez
  • J. G. Frerichs
  • K. Joeris
  • K. Konstantinov
  • T. Scheper

Research Organisations

External Research Organisations

  • Universidad de Costa Rica
  • Bayer Corporation - USA
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Details

Original languageEnglish
Title of host publication2006 IEEE International Conference on Acoustics, Speech, and Signal Processing
Subtitle of host publicationProceedings
Pages581-584
Volume5
Publication statusPublished - 24 Jul 2006
Event2006 IEEE International Conference on Acoustics, Speech and Signal Processing, ICASSP 2006 - Toulouse, France
Duration: 14 May 200619 May 2006

Publication series

NameICASSP, IEEE International Conference on Acoustics, Speech and Signal Processing - Proceedings
ISSN (Print)1520-6149

Abstract

In this contribution, the Lee and Rosenfeld's local Shape From Shading (SFS) algorithm, the Tsai and Shah's linear SFS algorithm and the Bichsel and Pentland's propagation SFS algorithm are investigated with the aim of selecting the most suitable for three-dimensional shape estimation of clusters of mammalian Baby Hamster Kidney cells (BHK cells) from an intensity image captured by an in-situ microscope in an industrial mammalian cell culture process. All three were implemented and tested using several thousand intensity images captured under varying experimental conditions. The Bichsel and Pentland's SFS algorithm was finally selected as the most suitable algorithm for three-dimensional shape estimation of BHK cell clusters. It is fast and provides less noise and more detailed depth estimates and therefore the best overall performance.

ASJC Scopus subject areas

Cite this

Three-dimensional shape estimation of BHK cell clusters from a still image based on shape from shading for in-situ microscopy. / Martinez, G.; Frerichs, J. G.; Joeris, K. et al.
2006 IEEE International Conference on Acoustics, Speech, and Signal Processing: Proceedings. Vol. 5 2006. p. 581-584 (ICASSP, IEEE International Conference on Acoustics, Speech and Signal Processing - Proceedings).

Research output: Chapter in book/report/conference proceedingConference contributionResearchpeer review

Martinez, G, Frerichs, JG, Joeris, K, Konstantinov, K & Scheper, T 2006, Three-dimensional shape estimation of BHK cell clusters from a still image based on shape from shading for in-situ microscopy. in 2006 IEEE International Conference on Acoustics, Speech, and Signal Processing: Proceedings. vol. 5, ICASSP, IEEE International Conference on Acoustics, Speech and Signal Processing - Proceedings, pp. 581-584, 2006 IEEE International Conference on Acoustics, Speech and Signal Processing, ICASSP 2006, Toulouse, France, 14 May 2006. https://doi.org/10.1109/ICASSP.2006.1660409
Martinez, G., Frerichs, J. G., Joeris, K., Konstantinov, K., & Scheper, T. (2006). Three-dimensional shape estimation of BHK cell clusters from a still image based on shape from shading for in-situ microscopy. In 2006 IEEE International Conference on Acoustics, Speech, and Signal Processing: Proceedings (Vol. 5, pp. 581-584). (ICASSP, IEEE International Conference on Acoustics, Speech and Signal Processing - Proceedings). https://doi.org/10.1109/ICASSP.2006.1660409
Martinez G, Frerichs JG, Joeris K, Konstantinov K, Scheper T. Three-dimensional shape estimation of BHK cell clusters from a still image based on shape from shading for in-situ microscopy. In 2006 IEEE International Conference on Acoustics, Speech, and Signal Processing: Proceedings. Vol. 5. 2006. p. 581-584. (ICASSP, IEEE International Conference on Acoustics, Speech and Signal Processing - Proceedings). doi: 10.1109/ICASSP.2006.1660409
Martinez, G. ; Frerichs, J. G. ; Joeris, K. et al. / Three-dimensional shape estimation of BHK cell clusters from a still image based on shape from shading for in-situ microscopy. 2006 IEEE International Conference on Acoustics, Speech, and Signal Processing: Proceedings. Vol. 5 2006. pp. 581-584 (ICASSP, IEEE International Conference on Acoustics, Speech and Signal Processing - Proceedings).
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