Details
Original language | English |
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Title of host publication | Ophthalmic Technologies XX |
Publication status | Published - 2 Mar 2010 |
Externally published | Yes |
Event | Ophthalmic Technologies XX - San Francisco, CA, United States Duration: 23 Jan 2010 → 25 Jan 2010 |
Publication series
Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
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Volume | 7550 |
ISSN (Print) | 1605-7422 |
Abstract
Cross-linking of stromal collagen with Riboflavin and UVA radiation is an alternative treatment of keratoconus. After the cross-linking a wound healing process starts with the regeneration of the abraded epithelial layer and the stromal keratocyte-network. To clarify possible side effects by visualization we established an imaging platform for the multimodal three-dimensional imaging of the cornea and looked for differences between normal and cross-linked rabbit corneae. The microscopy system utilizes femtosecond laser light for two photon excitation of autofluorescent metabolic compounds, second harmonic imaging in forward and backward direction for the study of stromal collagen-I structure and confocal detection of the backscattered femtosecond laser light for cell detection. Preliminary results show signatures of treatment 5 weeks after the intervention in all imaging modalities.
Keywords
- Confocal laser scanning microscopy, Cornea, Cross-linking, Keratoconus, Nonlinear microscopy, Second harmonic imaging, Two photon excited fluorescence
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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Ophthalmic Technologies XX. 2010. 755002 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7550).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Three-dimensional multimodal microscopy of rabbit cornea after cross-linking treatment
AU - Krüger, Alexander
AU - Hovakimyan, Marina
AU - Ramírez, D. F.
AU - Lorbeer, R. A.
AU - Kröger, Matthias
AU - Stachs, Oliver
AU - Wree, Andreas
AU - Guthoff, Rudolf Friedrich
AU - Lubatschowski, Holger
AU - Heisterkamp, Alexander
PY - 2010/3/2
Y1 - 2010/3/2
N2 - Cross-linking of stromal collagen with Riboflavin and UVA radiation is an alternative treatment of keratoconus. After the cross-linking a wound healing process starts with the regeneration of the abraded epithelial layer and the stromal keratocyte-network. To clarify possible side effects by visualization we established an imaging platform for the multimodal three-dimensional imaging of the cornea and looked for differences between normal and cross-linked rabbit corneae. The microscopy system utilizes femtosecond laser light for two photon excitation of autofluorescent metabolic compounds, second harmonic imaging in forward and backward direction for the study of stromal collagen-I structure and confocal detection of the backscattered femtosecond laser light for cell detection. Preliminary results show signatures of treatment 5 weeks after the intervention in all imaging modalities.
AB - Cross-linking of stromal collagen with Riboflavin and UVA radiation is an alternative treatment of keratoconus. After the cross-linking a wound healing process starts with the regeneration of the abraded epithelial layer and the stromal keratocyte-network. To clarify possible side effects by visualization we established an imaging platform for the multimodal three-dimensional imaging of the cornea and looked for differences between normal and cross-linked rabbit corneae. The microscopy system utilizes femtosecond laser light for two photon excitation of autofluorescent metabolic compounds, second harmonic imaging in forward and backward direction for the study of stromal collagen-I structure and confocal detection of the backscattered femtosecond laser light for cell detection. Preliminary results show signatures of treatment 5 weeks after the intervention in all imaging modalities.
KW - Confocal laser scanning microscopy
KW - Cornea
KW - Cross-linking
KW - Keratoconus
KW - Nonlinear microscopy
KW - Second harmonic imaging
KW - Two photon excited fluorescence
UR - http://www.scopus.com/inward/record.url?scp=79953067159&partnerID=8YFLogxK
U2 - 10.1117/12.842282
DO - 10.1117/12.842282
M3 - Conference contribution
AN - SCOPUS:79953067159
SN - 9780819479464
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Ophthalmic Technologies XX
T2 - Ophthalmic Technologies XX
Y2 - 23 January 2010 through 25 January 2010
ER -