Details
| Original language | English |
|---|---|
| Title of host publication | Photonic Therapeutics and Diagnostics XII |
| Publisher | SPIE |
| ISBN (electronic) | 9781628419245 |
| Publication status | Published - 29 Feb 2016 |
| Event | Photonic Therapeutics and Diagnostics XII - San Francisco, United States Duration: 13 Feb 2016 → 14 Feb 2016 |
Publication series
| Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
|---|---|
| Volume | 9689 |
| ISSN (Print) | 1605-7422 |
Abstract
The presented study focuses on the application of scanning laser optical tomography (SLOT) for non-destructive visualization of anatomical structures inside the human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique, which allows for tomographic imaging of the internal structure of transparent large-scale specimens (up to 1 cm3). Thus, in the field of otology this technique is best convenient for an ex vivo study of the inner ear anatomy. For this purpose, the preparation before imaging comprises mechanically assisted decalcification, dehydration as well as optical clearing of the cochlea samples. Here, we demonstrate results of SLOT visualizing hard and soft tissue structures of the human cochlea with an optical resolution in the micrometer range using absorption and autofluorescence as contrast mechanisms. Furthermore, we compare our results with the method of X-ray micro tomography (micro-CT, μCT) as clinical gold standard which is based only on absorption. In general, SLOT can provide the advantage of covering all contrast mechanisms known from other light microscopy techniques, such as fluorescence or scattering. For this reason, a protocol for antibody staining has been developed, which additionally enables selective mapping of cellular structures within the cochlea. Thus, we present results of SLOT imaging rodent cochleae showing specific anatomical structures such as hair cells and neurofilament via fluorescence. In conclusion, the presented study has shown that SLOT is an ideally suited tool in the field of otology for in toto visualization of the inner ear microstructure.
Keywords
- 3D microscopy, antibody staining, cochlea, inner ear, optical clearing, scanning laser optical tomography, SLOT, tomography, μCT
ASJC Scopus subject areas
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Biomaterials
- Physics and Astronomy(all)
- Atomic and Molecular Physics, and Optics
- Medicine(all)
- Radiology Nuclear Medicine and imaging
Cite this
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Photonic Therapeutics and Diagnostics XII. SPIE, 2016. 96892H (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9689).
Research output: Chapter in book/report/conference proceeding › Conference contribution › Research › peer review
}
TY - GEN
T1 - Three-dimensional imaging of intracochlear tissue by scanning laser optical tomography (SLOT)
AU - Tinne, Nadine
AU - Nolte, Lena
AU - Antonopoulos, Georgios Christian
AU - Schulze, Jennifer
AU - Andrade, J.
AU - Heisterkamp, Alexander
AU - Meyer, H.
AU - Warnecke, A.
AU - Majdani, O.
AU - Ripken, T.
PY - 2016/2/29
Y1 - 2016/2/29
N2 - The presented study focuses on the application of scanning laser optical tomography (SLOT) for non-destructive visualization of anatomical structures inside the human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique, which allows for tomographic imaging of the internal structure of transparent large-scale specimens (up to 1 cm3). Thus, in the field of otology this technique is best convenient for an ex vivo study of the inner ear anatomy. For this purpose, the preparation before imaging comprises mechanically assisted decalcification, dehydration as well as optical clearing of the cochlea samples. Here, we demonstrate results of SLOT visualizing hard and soft tissue structures of the human cochlea with an optical resolution in the micrometer range using absorption and autofluorescence as contrast mechanisms. Furthermore, we compare our results with the method of X-ray micro tomography (micro-CT, μCT) as clinical gold standard which is based only on absorption. In general, SLOT can provide the advantage of covering all contrast mechanisms known from other light microscopy techniques, such as fluorescence or scattering. For this reason, a protocol for antibody staining has been developed, which additionally enables selective mapping of cellular structures within the cochlea. Thus, we present results of SLOT imaging rodent cochleae showing specific anatomical structures such as hair cells and neurofilament via fluorescence. In conclusion, the presented study has shown that SLOT is an ideally suited tool in the field of otology for in toto visualization of the inner ear microstructure.
AB - The presented study focuses on the application of scanning laser optical tomography (SLOT) for non-destructive visualization of anatomical structures inside the human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique, which allows for tomographic imaging of the internal structure of transparent large-scale specimens (up to 1 cm3). Thus, in the field of otology this technique is best convenient for an ex vivo study of the inner ear anatomy. For this purpose, the preparation before imaging comprises mechanically assisted decalcification, dehydration as well as optical clearing of the cochlea samples. Here, we demonstrate results of SLOT visualizing hard and soft tissue structures of the human cochlea with an optical resolution in the micrometer range using absorption and autofluorescence as contrast mechanisms. Furthermore, we compare our results with the method of X-ray micro tomography (micro-CT, μCT) as clinical gold standard which is based only on absorption. In general, SLOT can provide the advantage of covering all contrast mechanisms known from other light microscopy techniques, such as fluorescence or scattering. For this reason, a protocol for antibody staining has been developed, which additionally enables selective mapping of cellular structures within the cochlea. Thus, we present results of SLOT imaging rodent cochleae showing specific anatomical structures such as hair cells and neurofilament via fluorescence. In conclusion, the presented study has shown that SLOT is an ideally suited tool in the field of otology for in toto visualization of the inner ear microstructure.
KW - 3D microscopy
KW - antibody staining
KW - cochlea
KW - inner ear
KW - optical clearing
KW - scanning laser optical tomography
KW - SLOT
KW - tomography
KW - μCT
UR - http://www.scopus.com/inward/record.url?scp=84973397936&partnerID=8YFLogxK
U2 - 10.1117/12.2211066
DO - 10.1117/12.2211066
M3 - Conference contribution
AN - SCOPUS:84973397936
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Photonic Therapeutics and Diagnostics XII
PB - SPIE
T2 - Photonic Therapeutics and Diagnostics XII
Y2 - 13 February 2016 through 14 February 2016
ER -