Details
| Original language | English |
|---|---|
| Pages (from-to) | 275-87 |
| Number of pages | 13 |
| Journal | Journal of Biomaterials Applications |
| Volume | 22 |
| Issue number | 3 |
| Publication status | Published - 1 Nov 2007 |
Abstract
Two-photon polymerization technique was applied to generate three-dimensional (3D) scaffold-like structures using the photosensitive organic-inorganic hybrid polymer ORMOCER. The structures were studied with respect to potential applications as scaffold for tissue engineering. Cell counting and comet assay, respectively, demonstrated that doubling time and DNA strand breaks of CHO cells, GFSHR-17 granulosa cells, GM-7373 endothelial cells, and SH-SY5Y neuroblastoma cells were not affected by ORMOCER. ORMOCER related alteration of formation of tissue specific cell-to-cell adhesions like gap junctions was ruled out by double whole-cell patch-clamp technique. Additionally, growth of cells on the vertical surfaces of 3D structures composed of ORMOCER is shown.
Keywords
- Animals, Biocompatible Materials/chemistry, CHO Cells, Cell Adhesion/drug effects, Cell Communication/drug effects, Cell Line, Tumor, Cell Proliferation/drug effects, Ceramics/chemistry, Computer-Aided Design/trends, Cricetinae, Cricetulus, DNA Damage/drug effects, Gap Junctions/drug effects, Guided Tissue Regeneration/instrumentation, Humans, Lasers, Materials Testing/methods, Membrane Potentials/drug effects, Organically Modified Ceramics, Photochemistry/methods, Polymers/chemistry, Silanes/chemistry, Tissue Engineering/instrumentation, Tissue Scaffolds
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In: Journal of Biomaterials Applications, Vol. 22, No. 3, 01.11.2007, p. 275-87.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Three-dimensional cell growth on structures fabricated from ORMOCER by two-photon polymerization technique
AU - Schlie, Sabrina
AU - Ngezahayo, Anaclet
AU - Ovsianikov, Aleksandr
AU - Fabian, Tilman
AU - Kolb, Hans-Albert
AU - Haferkamp, Heinz
AU - Chichkov, Boris N
PY - 2007/11/1
Y1 - 2007/11/1
N2 - Two-photon polymerization technique was applied to generate three-dimensional (3D) scaffold-like structures using the photosensitive organic-inorganic hybrid polymer ORMOCER. The structures were studied with respect to potential applications as scaffold for tissue engineering. Cell counting and comet assay, respectively, demonstrated that doubling time and DNA strand breaks of CHO cells, GFSHR-17 granulosa cells, GM-7373 endothelial cells, and SH-SY5Y neuroblastoma cells were not affected by ORMOCER. ORMOCER related alteration of formation of tissue specific cell-to-cell adhesions like gap junctions was ruled out by double whole-cell patch-clamp technique. Additionally, growth of cells on the vertical surfaces of 3D structures composed of ORMOCER is shown.
AB - Two-photon polymerization technique was applied to generate three-dimensional (3D) scaffold-like structures using the photosensitive organic-inorganic hybrid polymer ORMOCER. The structures were studied with respect to potential applications as scaffold for tissue engineering. Cell counting and comet assay, respectively, demonstrated that doubling time and DNA strand breaks of CHO cells, GFSHR-17 granulosa cells, GM-7373 endothelial cells, and SH-SY5Y neuroblastoma cells were not affected by ORMOCER. ORMOCER related alteration of formation of tissue specific cell-to-cell adhesions like gap junctions was ruled out by double whole-cell patch-clamp technique. Additionally, growth of cells on the vertical surfaces of 3D structures composed of ORMOCER is shown.
KW - Animals
KW - Biocompatible Materials/chemistry
KW - CHO Cells
KW - Cell Adhesion/drug effects
KW - Cell Communication/drug effects
KW - Cell Line, Tumor
KW - Cell Proliferation/drug effects
KW - Ceramics/chemistry
KW - Computer-Aided Design/trends
KW - Cricetinae
KW - Cricetulus
KW - DNA Damage/drug effects
KW - Gap Junctions/drug effects
KW - Guided Tissue Regeneration/instrumentation
KW - Humans
KW - Lasers
KW - Materials Testing/methods
KW - Membrane Potentials/drug effects
KW - Organically Modified Ceramics
KW - Photochemistry/methods
KW - Polymers/chemistry
KW - Silanes/chemistry
KW - Tissue Engineering/instrumentation
KW - Tissue Scaffolds
U2 - 10.1177/0885328207077590
DO - 10.1177/0885328207077590
M3 - Article
C2 - 17494962
VL - 22
SP - 275
EP - 287
JO - Journal of Biomaterials Applications
JF - Journal of Biomaterials Applications
SN - 0885-3282
IS - 3
ER -