Details
| Original language | English |
|---|---|
| Pages (from-to) | 625-631 |
| Number of pages | 7 |
| Journal | Enzyme and microbial technology |
| Volume | 9 |
| Issue number | 10 |
| Publication status | Published - Oct 1987 |
Abstract
Three different examples of enzyme emulsions are presented. The enzymes are immobilized in liquid surfactant membranes. The effect of the organic membrane phase is discussed as well as the influence of the membrane composition on the transport of substrates and products through the membrane. An enzyme emulsion system for the production of l-leucine with continuous co-factor regeneration is described. It is not necessary to increase the molecular weight of the co-factor by linking it to a soluble high molecular weight compound (e.g., PEG), since the coenzyme cannot pass the liquid membrane without a suitable carrier. Also, a product (6-APA) can be enriched in the internal phase of the liquid membrane. The separation effect is not based on differences in molecular weight, but on the chemical behavior of the substances to be separated.
Keywords
- enzyme bioconversion, enzyme emulsions, enzyme/coenzyme systems, L-leucine, liquid membrane reactors
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Chemical Engineering(all)
- Bioengineering
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Enzyme and microbial technology, Vol. 9, No. 10, 10.1987, p. 625-631.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - Three different examples of enzymatic bioconversion in liquid membrane reactors
AU - Scheper, T.
AU - Likidis, Z.
AU - Makryaleas, K.
AU - Nowottny, Ch
AU - Schügerl, K.
PY - 1987/10
Y1 - 1987/10
N2 - Three different examples of enzyme emulsions are presented. The enzymes are immobilized in liquid surfactant membranes. The effect of the organic membrane phase is discussed as well as the influence of the membrane composition on the transport of substrates and products through the membrane. An enzyme emulsion system for the production of l-leucine with continuous co-factor regeneration is described. It is not necessary to increase the molecular weight of the co-factor by linking it to a soluble high molecular weight compound (e.g., PEG), since the coenzyme cannot pass the liquid membrane without a suitable carrier. Also, a product (6-APA) can be enriched in the internal phase of the liquid membrane. The separation effect is not based on differences in molecular weight, but on the chemical behavior of the substances to be separated.
AB - Three different examples of enzyme emulsions are presented. The enzymes are immobilized in liquid surfactant membranes. The effect of the organic membrane phase is discussed as well as the influence of the membrane composition on the transport of substrates and products through the membrane. An enzyme emulsion system for the production of l-leucine with continuous co-factor regeneration is described. It is not necessary to increase the molecular weight of the co-factor by linking it to a soluble high molecular weight compound (e.g., PEG), since the coenzyme cannot pass the liquid membrane without a suitable carrier. Also, a product (6-APA) can be enriched in the internal phase of the liquid membrane. The separation effect is not based on differences in molecular weight, but on the chemical behavior of the substances to be separated.
KW - enzyme bioconversion
KW - enzyme emulsions
KW - enzyme/coenzyme systems
KW - L-leucine
KW - liquid membrane reactors
UR - http://www.scopus.com/inward/record.url?scp=0023440242&partnerID=8YFLogxK
U2 - 10.1016/0141-0229(87)90117-7
DO - 10.1016/0141-0229(87)90117-7
M3 - Article
AN - SCOPUS:0023440242
VL - 9
SP - 625
EP - 631
JO - Enzyme and microbial technology
JF - Enzyme and microbial technology
SN - 0141-0229
IS - 10
ER -