Details
| Original language | English |
|---|---|
| Pages (from-to) | 173-181 |
| Number of pages | 9 |
| Journal | PLANT BREEDING |
| Volume | 106 |
| Issue number | 3 |
| Publication status | Published - Apr 1991 |
Abstract
The germplasm of the potato (S. tuberosum ssp. tuberosum) has been modified since the beginning of this century by breeders who introgressed important agronomic traits, for example disease resistance genes, from several wild and cultivated Solanum species of the Americas. In this paper we show that the Rflp analysis of potato‐breeding material can be used to detect chromosomal regions descended from more‐distantly related Solanum species. The Rflp patterns of individuals of ten Solanum species, ten breeding lines of S. tuberosum ssp. tuberosum and the cultivated variety ‘Bintje’ were analyzed. Rflp data for each of eight single loci of known genomic position were used for the computation of locus‐specific phenograms by distance matrix methods. Several of the potato‐breeding lines deviated clearly from the clustered species S. tuberosum, S. stenotomum and S. canasense for one or more of the loci considered. These deviations indicated the presence of “exotic” germplasm at a particular locus. The possibility of detecting such germplasm has implications for mapping the agronomic traits for which the wild species were introgressed and which may still be linked to “foreign” chromosome fragments.
Keywords
- distance matrix, germplasm, introgression, RFLP, Solanum tuberosum
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: PLANT BREEDING, Vol. 106, No. 3, 04.1991, p. 173-181.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The Use of RFLPs (Restriction Fragment Length Polymorphisms) Detects Germplasm Introgressions from Wild Species into Potato (Solanum tuberosum ssp. tuberosum) Breeding Lines
AU - Debener, T.
AU - Salamini, F.
AU - Gebhardt, Christiane
PY - 1991/4
Y1 - 1991/4
N2 - The germplasm of the potato (S. tuberosum ssp. tuberosum) has been modified since the beginning of this century by breeders who introgressed important agronomic traits, for example disease resistance genes, from several wild and cultivated Solanum species of the Americas. In this paper we show that the Rflp analysis of potato‐breeding material can be used to detect chromosomal regions descended from more‐distantly related Solanum species. The Rflp patterns of individuals of ten Solanum species, ten breeding lines of S. tuberosum ssp. tuberosum and the cultivated variety ‘Bintje’ were analyzed. Rflp data for each of eight single loci of known genomic position were used for the computation of locus‐specific phenograms by distance matrix methods. Several of the potato‐breeding lines deviated clearly from the clustered species S. tuberosum, S. stenotomum and S. canasense for one or more of the loci considered. These deviations indicated the presence of “exotic” germplasm at a particular locus. The possibility of detecting such germplasm has implications for mapping the agronomic traits for which the wild species were introgressed and which may still be linked to “foreign” chromosome fragments.
AB - The germplasm of the potato (S. tuberosum ssp. tuberosum) has been modified since the beginning of this century by breeders who introgressed important agronomic traits, for example disease resistance genes, from several wild and cultivated Solanum species of the Americas. In this paper we show that the Rflp analysis of potato‐breeding material can be used to detect chromosomal regions descended from more‐distantly related Solanum species. The Rflp patterns of individuals of ten Solanum species, ten breeding lines of S. tuberosum ssp. tuberosum and the cultivated variety ‘Bintje’ were analyzed. Rflp data for each of eight single loci of known genomic position were used for the computation of locus‐specific phenograms by distance matrix methods. Several of the potato‐breeding lines deviated clearly from the clustered species S. tuberosum, S. stenotomum and S. canasense for one or more of the loci considered. These deviations indicated the presence of “exotic” germplasm at a particular locus. The possibility of detecting such germplasm has implications for mapping the agronomic traits for which the wild species were introgressed and which may still be linked to “foreign” chromosome fragments.
KW - distance matrix
KW - germplasm
KW - introgression
KW - RFLP
KW - Solanum tuberosum
UR - http://www.scopus.com/inward/record.url?scp=84989035999&partnerID=8YFLogxK
U2 - 10.1111/j.1439-0523.1991.tb00498.x
DO - 10.1111/j.1439-0523.1991.tb00498.x
M3 - Article
AN - SCOPUS:84989035999
VL - 106
SP - 173
EP - 181
JO - PLANT BREEDING
JF - PLANT BREEDING
SN - 0179-9541
IS - 3
ER -