Details
| Original language | English |
|---|---|
| Pages (from-to) | 116-120 |
| Number of pages | 5 |
| Journal | Current Directions in Biomedical Engineering |
| Volume | 1 |
| Issue number | 1 |
| Publication status | Published - Sept 2015 |
Abstract
Today researchers look for a possibility to keep cells for a long time without losing their viability. For that cryopreservation is often used. In this process it is necessary that the cells are not destroyed so cryoprotective agents (CPA) are needed. At the moment 5 to 10 % dimethylsulfoxide (DMSO) is mostly used, but this chemical is cytotoxic to the cells. So an alternative is needed. In this work experiments are made with an icebindingprotein (IBP) of Hypogastrura harveyi, as an alternative to DMSO. It was shown in previous studies that this protein isn't cytotoxic for the cells, with crude extract and purified inclusion bodies it even seems that the mixtures have a positive effect on growth and proliferation. As a first step the protein was produced heterologous in E. coli and then the crude extract and the purified inclusion bodies were used for experiments on the influence of the IBP on the cryopreservation of mesenchymal stem cells from the common marmoset monkey Callithrix jacchus. In the process it was found that the protein could not replace DMSO completely. But it was possible to show that the DMSO-concentration can be reduced by adding the IBP.
Keywords
- cryopreservation, DMSO, Hypogastrura harveyi, icebindingprotein, mesenchymal stem cells
ASJC Scopus subject areas
- Engineering(all)
- Biomedical Engineering
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In: Current Directions in Biomedical Engineering, Vol. 1, No. 1, 09.2015, p. 116-120.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The use of an icebindingprotein out of the snowflea Hypogastrura harveyi as a cryoprotectant in the cryopreservation of mesenchymal stem cells
AU - Vierthaler, M.
AU - Reinard, T.
AU - Glasmacher, B.
AU - Hofmann, N.
PY - 2015/9
Y1 - 2015/9
N2 - Today researchers look for a possibility to keep cells for a long time without losing their viability. For that cryopreservation is often used. In this process it is necessary that the cells are not destroyed so cryoprotective agents (CPA) are needed. At the moment 5 to 10 % dimethylsulfoxide (DMSO) is mostly used, but this chemical is cytotoxic to the cells. So an alternative is needed. In this work experiments are made with an icebindingprotein (IBP) of Hypogastrura harveyi, as an alternative to DMSO. It was shown in previous studies that this protein isn't cytotoxic for the cells, with crude extract and purified inclusion bodies it even seems that the mixtures have a positive effect on growth and proliferation. As a first step the protein was produced heterologous in E. coli and then the crude extract and the purified inclusion bodies were used for experiments on the influence of the IBP on the cryopreservation of mesenchymal stem cells from the common marmoset monkey Callithrix jacchus. In the process it was found that the protein could not replace DMSO completely. But it was possible to show that the DMSO-concentration can be reduced by adding the IBP.
AB - Today researchers look for a possibility to keep cells for a long time without losing their viability. For that cryopreservation is often used. In this process it is necessary that the cells are not destroyed so cryoprotective agents (CPA) are needed. At the moment 5 to 10 % dimethylsulfoxide (DMSO) is mostly used, but this chemical is cytotoxic to the cells. So an alternative is needed. In this work experiments are made with an icebindingprotein (IBP) of Hypogastrura harveyi, as an alternative to DMSO. It was shown in previous studies that this protein isn't cytotoxic for the cells, with crude extract and purified inclusion bodies it even seems that the mixtures have a positive effect on growth and proliferation. As a first step the protein was produced heterologous in E. coli and then the crude extract and the purified inclusion bodies were used for experiments on the influence of the IBP on the cryopreservation of mesenchymal stem cells from the common marmoset monkey Callithrix jacchus. In the process it was found that the protein could not replace DMSO completely. But it was possible to show that the DMSO-concentration can be reduced by adding the IBP.
KW - cryopreservation
KW - DMSO
KW - Hypogastrura harveyi
KW - icebindingprotein
KW - mesenchymal stem cells
UR - http://www.scopus.com/inward/record.url?scp=85111971991&partnerID=8YFLogxK
U2 - 10.1515/cdbme-2015-0030
DO - 10.1515/cdbme-2015-0030
M3 - Article
AN - SCOPUS:85111971991
VL - 1
SP - 116
EP - 120
JO - Current Directions in Biomedical Engineering
JF - Current Directions in Biomedical Engineering
IS - 1
ER -