Details
| Original language | English |
|---|---|
| Pages (from-to) | 314-326 |
| Number of pages | 13 |
| Journal | Cryo-letters |
| Volume | 44 |
| Issue number | 6 |
| Publication status | Published - Nov 2023 |
Abstract
The process of freezing biological material at extremely low temperatures is known as cryopreservation. To ensure the preservation of cells and tissues over an extended period of time, low temperatures are applied since biological processes, including the biochemical ones, come to a halt under cryogenic conditions and thus it is possible to maintain their structural and functional integrity. The field of cryopreservation gained more prominence in the 20th century and emerged as an unavoidable technology for different applications such as cell therapy, tissue engineering, or assisted fertilization. In this work we provide an overview of various technologies in the field of cryotechnology with regard to the freezing, storage and thawing of living cells. The first part covers the freezing process, starting with cryoprotective agents regarding their protection mechanisms and compositions, passing by cryoimaging, micro-fluidic systems, and the currently available freezing and biobanking equipment. The second part focusses on the thawing process as well as the hypothermic preservation for the short-term storage of biological materials and constructs.
Keywords
- biobanking, CPA, freezing, hypothermal storage, microfluidic, nanowarming
ASJC Scopus subject areas
- Medicine(all)
- Anatomy
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
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In: Cryo-letters, Vol. 44, No. 6, 11.2023, p. 314-326.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The Technology in Cryotechnology
AU - Hagedorn, Janina
AU - Alkurdi, Ghiath
AU - Barker, Sven Alexander
AU - Brunotte, Ricarda
AU - Deeb, Tarek
AU - Hubenia, Oleksandra
AU - Khayyat, Diaa
AU - Leal-Marin, Sara
AU - Rittinghaus, Tim
AU - Glasmacher, Birgit
PY - 2023/11
Y1 - 2023/11
N2 - The process of freezing biological material at extremely low temperatures is known as cryopreservation. To ensure the preservation of cells and tissues over an extended period of time, low temperatures are applied since biological processes, including the biochemical ones, come to a halt under cryogenic conditions and thus it is possible to maintain their structural and functional integrity. The field of cryopreservation gained more prominence in the 20th century and emerged as an unavoidable technology for different applications such as cell therapy, tissue engineering, or assisted fertilization. In this work we provide an overview of various technologies in the field of cryotechnology with regard to the freezing, storage and thawing of living cells. The first part covers the freezing process, starting with cryoprotective agents regarding their protection mechanisms and compositions, passing by cryoimaging, micro-fluidic systems, and the currently available freezing and biobanking equipment. The second part focusses on the thawing process as well as the hypothermic preservation for the short-term storage of biological materials and constructs.
AB - The process of freezing biological material at extremely low temperatures is known as cryopreservation. To ensure the preservation of cells and tissues over an extended period of time, low temperatures are applied since biological processes, including the biochemical ones, come to a halt under cryogenic conditions and thus it is possible to maintain their structural and functional integrity. The field of cryopreservation gained more prominence in the 20th century and emerged as an unavoidable technology for different applications such as cell therapy, tissue engineering, or assisted fertilization. In this work we provide an overview of various technologies in the field of cryotechnology with regard to the freezing, storage and thawing of living cells. The first part covers the freezing process, starting with cryoprotective agents regarding their protection mechanisms and compositions, passing by cryoimaging, micro-fluidic systems, and the currently available freezing and biobanking equipment. The second part focusses on the thawing process as well as the hypothermic preservation for the short-term storage of biological materials and constructs.
KW - biobanking
KW - CPA
KW - freezing
KW - hypothermal storage
KW - microfluidic
KW - nanowarming
UR - http://www.scopus.com/inward/record.url?scp=85181515308&partnerID=8YFLogxK
U2 - 10.54680/fr23610110112
DO - 10.54680/fr23610110112
M3 - Article
AN - SCOPUS:85181515308
VL - 44
SP - 314
EP - 326
JO - Cryo-letters
JF - Cryo-letters
SN - 0143-2044
IS - 6
ER -