Details
| Original language | English |
|---|---|
| Pages (from-to) | 4832-4838 |
| Number of pages | 7 |
| Journal | PROTEOMICS |
| Volume | 5 |
| Issue number | 18 |
| Publication status | Published - 12 Dec 2005 |
Abstract
Due to their unique capability to attack lignified biopolymers, extracellular enzymes of white-rot fungi enjoy an increasing interest in various fields of white biotechnology. The edible fungus Pleurotus sapidus was selected as a model organism for the analysis of the secretome by means of 2-DE. For enzyme production, the fungus was grown in submerged cultures either on peanut shells or on glass wool as a carrier material. Identification of the secreted enzymes was performed by tryptic digestion, ESI-MS/MS ab initio sequencing, and homology searches against public databases. The spectrum of secreted enzymes comprised various types of hydrolases and lignolytic enzymes of the manganese peroxidase/versatile peroxidase family. While peptidases were secreted mainly by the cultures grown on peanut shells, versatile peroxidase type enzymes dominated in the cultures grown on glass wool.
Keywords
- 2-D electrophoresis, Basidiomycete, Extracellular enzymes, Fungi
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Molecular Biology
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In: PROTEOMICS, Vol. 5, No. 18, 12.12.2005, p. 4832-4838.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The secretome of Pleurotus sapidus
AU - Zorn, Holger
AU - Peters, Thilo
AU - Nimtz, Manfred
AU - Berger, Ralf G.
PY - 2005/12/12
Y1 - 2005/12/12
N2 - Due to their unique capability to attack lignified biopolymers, extracellular enzymes of white-rot fungi enjoy an increasing interest in various fields of white biotechnology. The edible fungus Pleurotus sapidus was selected as a model organism for the analysis of the secretome by means of 2-DE. For enzyme production, the fungus was grown in submerged cultures either on peanut shells or on glass wool as a carrier material. Identification of the secreted enzymes was performed by tryptic digestion, ESI-MS/MS ab initio sequencing, and homology searches against public databases. The spectrum of secreted enzymes comprised various types of hydrolases and lignolytic enzymes of the manganese peroxidase/versatile peroxidase family. While peptidases were secreted mainly by the cultures grown on peanut shells, versatile peroxidase type enzymes dominated in the cultures grown on glass wool.
AB - Due to their unique capability to attack lignified biopolymers, extracellular enzymes of white-rot fungi enjoy an increasing interest in various fields of white biotechnology. The edible fungus Pleurotus sapidus was selected as a model organism for the analysis of the secretome by means of 2-DE. For enzyme production, the fungus was grown in submerged cultures either on peanut shells or on glass wool as a carrier material. Identification of the secreted enzymes was performed by tryptic digestion, ESI-MS/MS ab initio sequencing, and homology searches against public databases. The spectrum of secreted enzymes comprised various types of hydrolases and lignolytic enzymes of the manganese peroxidase/versatile peroxidase family. While peptidases were secreted mainly by the cultures grown on peanut shells, versatile peroxidase type enzymes dominated in the cultures grown on glass wool.
KW - 2-D electrophoresis
KW - Basidiomycete
KW - Extracellular enzymes
KW - Fungi
UR - http://www.scopus.com/inward/record.url?scp=28944455667&partnerID=8YFLogxK
U2 - 10.1002/pmic.200500015
DO - 10.1002/pmic.200500015
M3 - Article
C2 - 16281184
AN - SCOPUS:28944455667
VL - 5
SP - 4832
EP - 4838
JO - PROTEOMICS
JF - PROTEOMICS
SN - 1615-9853
IS - 18
ER -