Details
| Original language | English |
|---|---|
| Pages (from-to) | 10934-10937 |
| Number of pages | 4 |
| Journal | Chemical communications |
| Volume | 56 |
| Issue number | 74 |
| Publication status | Published - 21 Sept 2020 |
Abstract
Sorbicillinoids are a large family of fungal secondary metabolites with a diverse range of structures and numerous bioactivites, some of which have pharmaceutical potential. The flavin-dependent monooxygenase SorD fromPenicillium chrysogenum(PcSorD) utilizes sorbicillinol to catalyze a broad scope of reactions: formation of oxosorbicillinol and epoxysorbicillinol; intermolecular Diels-Alder and Michael-addition dimerization reactions; and dimerization of a sorbicillinol derivative with oxosorbicillinol. PcSorD shares only 18.3% sequence identity with SorD fromTrichoderma reesei(TrSorD) and yet unexpectedly catalyzes many of the same reactions, however, the formation of oxosorbicillinol and bisvertinolone by PcSorD extends the range of reactions catalyzed by a single enzyme. Phylogenetic analysis indicates that PcSorD and TrSorD bind the flavin cofactor covalently butviadifferent residues and point mutations confirm these residues are essential for activity.
ASJC Scopus subject areas
- Chemical Engineering(all)
- Catalysis
- Materials Science(all)
- Electronic, Optical and Magnetic Materials
- Materials Science(all)
- Ceramics and Composites
- Chemistry(all)
- Materials Science(all)
- Surfaces, Coatings and Films
- Materials Science(all)
- Metals and Alloys
- Materials Science(all)
- Materials Chemistry
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In: Chemical communications, Vol. 56, No. 74, 21.09.2020, p. 10934-10937.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The same but different: multiple functions of the fungal flavin dependent monooxygenase SorD fromPenicillium chrysogenum
AU - Kahlert, Lukas
AU - Cox, Russell J.
AU - Skellam, Elizabeth
N1 - Funding Information: This work was supported by the German Research Foundation (DFG, INST 187/621-1, INST 187/686-1). LK was funded by DFG (CO 1328/5-1).
PY - 2020/9/21
Y1 - 2020/9/21
N2 - Sorbicillinoids are a large family of fungal secondary metabolites with a diverse range of structures and numerous bioactivites, some of which have pharmaceutical potential. The flavin-dependent monooxygenase SorD fromPenicillium chrysogenum(PcSorD) utilizes sorbicillinol to catalyze a broad scope of reactions: formation of oxosorbicillinol and epoxysorbicillinol; intermolecular Diels-Alder and Michael-addition dimerization reactions; and dimerization of a sorbicillinol derivative with oxosorbicillinol. PcSorD shares only 18.3% sequence identity with SorD fromTrichoderma reesei(TrSorD) and yet unexpectedly catalyzes many of the same reactions, however, the formation of oxosorbicillinol and bisvertinolone by PcSorD extends the range of reactions catalyzed by a single enzyme. Phylogenetic analysis indicates that PcSorD and TrSorD bind the flavin cofactor covalently butviadifferent residues and point mutations confirm these residues are essential for activity.
AB - Sorbicillinoids are a large family of fungal secondary metabolites with a diverse range of structures and numerous bioactivites, some of which have pharmaceutical potential. The flavin-dependent monooxygenase SorD fromPenicillium chrysogenum(PcSorD) utilizes sorbicillinol to catalyze a broad scope of reactions: formation of oxosorbicillinol and epoxysorbicillinol; intermolecular Diels-Alder and Michael-addition dimerization reactions; and dimerization of a sorbicillinol derivative with oxosorbicillinol. PcSorD shares only 18.3% sequence identity with SorD fromTrichoderma reesei(TrSorD) and yet unexpectedly catalyzes many of the same reactions, however, the formation of oxosorbicillinol and bisvertinolone by PcSorD extends the range of reactions catalyzed by a single enzyme. Phylogenetic analysis indicates that PcSorD and TrSorD bind the flavin cofactor covalently butviadifferent residues and point mutations confirm these residues are essential for activity.
UR - http://www.scopus.com/inward/record.url?scp=85091191197&partnerID=8YFLogxK
U2 - 10.1039/d0cc03203d
DO - 10.1039/d0cc03203d
M3 - Article
VL - 56
SP - 10934
EP - 10937
JO - Chemical communications
JF - Chemical communications
SN - 1359-7345
IS - 74
ER -