Details
| Original language | English |
|---|---|
| Pages (from-to) | 1630-1644 |
| Number of pages | 15 |
| Journal | Plant Cell and Environment |
| Volume | 42 |
| Issue number | 5 |
| Early online date | 10 Jan 2019 |
| Publication status | Published - 12 Apr 2019 |
Abstract
Plant responses to abiotic stress include various modifications in amino acid metabolism. By using a hydroponic culture system, we systematically investigate modification in amino acid profiles and the proteome of Arabidopsis thaliana leaves during initial recovery from low water potential or high salinity. Both treatments elicited oxidative stress leading to a biphasic stress response during recovery. Degradation of highly abundant proteins such as subunits of photosystems and ribosomes contributed to an accumulation of free amino acids. Catabolic pathways for several low abundant amino acids were induced indicating their usage as an alternative respiratory substrate to compensate for the decreased photosynthesis. Our results demonstrate that rapid detoxification of potentially detrimental amino acids such as Lys is a priority during the initial stress recovery period. The content of Pro, which acts as a compatible osmolyte during stress, was adjusted by balancing its synthesis and catabolism both of which were induced both during and after stress treatments. The production of amino acid derived secondary metabolites was up-regulated specifically during the recovery period, and our dataset also indicates increased synthesis rates of the precursor amino acids. Overall, our results support a tight relationship between amino acid metabolism and stress responses.
Keywords
- Arabidopsis thaliana, drought, hydroponic culture, proteomics, salinity, Proline/metabolism, Oxidative Stress, Lysine/metabolism, Plant Leaves/metabolism, Stress, Physiological, Amino Acids/metabolism, Dehydration, Proteome/metabolism, Arabidopsis/physiology, Salt Stress, Arabidopsis Proteins/metabolism
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Physiology
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant Cell and Environment, Vol. 42, No. 5, 12.04.2019, p. 1630-1644.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The role of amino acid metabolism during abiotic stress release
AU - Batista-Silva, Willian
AU - Heinemann, Björn
AU - Rugen, Nils
AU - Nunes-Nesi, Adriano
AU - Araújo, Wagner L.
AU - Braun, Hans Peter
AU - Hildebrandt, Tatjana M.
N1 - Funding information: We thank Christa Ruppelt for excellent technical assistance in performing the root toxicity tests. This work was supported by the binational science funding programme “PROBRAL” of the Deutsche Akademische Austauschdienst (DAAD; funds provided by the Bundesministerium für Bildung und Forschung—BMBF) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Refs: Project?ID PROBRAL #423/14. Research fellowships granted by the National Council for Scientific and Technological Development (CNPq?Brazil) to A.N.N. and W.L.A. are gratefully acknowledged. We thank Christa Ruppelt for excellent technical assistance in performing the root toxicity tests. This work was supported by the binational science funding programme ?PROBRAL? of the Deutsche Akademische Austauschdienst (DAAD; funds provided by the Bundesministerium f?r Bildung und Forschung?BMBF) and the Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES), Refs: Project-ID PROBRAL #423/14. Research fellowships granted by the National Council for Scientific and Technological Development (CNPq-Brazil) to A.N.N. and W.L.A. are gratefully acknowledged.
PY - 2019/4/12
Y1 - 2019/4/12
N2 - Plant responses to abiotic stress include various modifications in amino acid metabolism. By using a hydroponic culture system, we systematically investigate modification in amino acid profiles and the proteome of Arabidopsis thaliana leaves during initial recovery from low water potential or high salinity. Both treatments elicited oxidative stress leading to a biphasic stress response during recovery. Degradation of highly abundant proteins such as subunits of photosystems and ribosomes contributed to an accumulation of free amino acids. Catabolic pathways for several low abundant amino acids were induced indicating their usage as an alternative respiratory substrate to compensate for the decreased photosynthesis. Our results demonstrate that rapid detoxification of potentially detrimental amino acids such as Lys is a priority during the initial stress recovery period. The content of Pro, which acts as a compatible osmolyte during stress, was adjusted by balancing its synthesis and catabolism both of which were induced both during and after stress treatments. The production of amino acid derived secondary metabolites was up-regulated specifically during the recovery period, and our dataset also indicates increased synthesis rates of the precursor amino acids. Overall, our results support a tight relationship between amino acid metabolism and stress responses.
AB - Plant responses to abiotic stress include various modifications in amino acid metabolism. By using a hydroponic culture system, we systematically investigate modification in amino acid profiles and the proteome of Arabidopsis thaliana leaves during initial recovery from low water potential or high salinity. Both treatments elicited oxidative stress leading to a biphasic stress response during recovery. Degradation of highly abundant proteins such as subunits of photosystems and ribosomes contributed to an accumulation of free amino acids. Catabolic pathways for several low abundant amino acids were induced indicating their usage as an alternative respiratory substrate to compensate for the decreased photosynthesis. Our results demonstrate that rapid detoxification of potentially detrimental amino acids such as Lys is a priority during the initial stress recovery period. The content of Pro, which acts as a compatible osmolyte during stress, was adjusted by balancing its synthesis and catabolism both of which were induced both during and after stress treatments. The production of amino acid derived secondary metabolites was up-regulated specifically during the recovery period, and our dataset also indicates increased synthesis rates of the precursor amino acids. Overall, our results support a tight relationship between amino acid metabolism and stress responses.
KW - Arabidopsis thaliana
KW - drought
KW - hydroponic culture
KW - proteomics
KW - salinity
KW - Proline/metabolism
KW - Oxidative Stress
KW - Lysine/metabolism
KW - Plant Leaves/metabolism
KW - Stress, Physiological
KW - Amino Acids/metabolism
KW - Dehydration
KW - Proteome/metabolism
KW - Arabidopsis/physiology
KW - Salt Stress
KW - Arabidopsis Proteins/metabolism
UR - http://www.scopus.com/inward/record.url?scp=85061281882&partnerID=8YFLogxK
U2 - 10.1111/pce.13518
DO - 10.1111/pce.13518
M3 - Article
C2 - 30632176
AN - SCOPUS:85061281882
VL - 42
SP - 1630
EP - 1644
JO - Plant Cell and Environment
JF - Plant Cell and Environment
SN - 0140-7791
IS - 5
ER -