Details
| Original language | English |
|---|---|
| Pages (from-to) | 759-772 |
| Number of pages | 14 |
| Journal | Plant molecular biology |
| Volume | 29 |
| Issue number | 4 |
| Publication status | Published - 1 Nov 1995 |
| Externally published | Yes |
Abstract
We recently reported on the broad bean gene VfENOD-GRP3 encoding a glycine-rich early nodulin. This gene was predominantly expressed in the interzone II-III region of Vicia faba root nodules. The VfENOD-GRP3 promoter contained several sequence motifs potentially involved in the regulation of gene expression. To investigate the molecular basis for the specific VfENOD-GRP3 expression, defined VfENOD-GRP3 promoter fragments were fused to an intron-containing gusAint gene. Agrobacterium rhizogenes ARqual strains carrying these fusions integrated into the TL DNA were used to generate hairy roots on Vicia hirsuta, which subsequently were nodulated. Histochemical analysis of transgenic nodules indicated that a strong gusAint expression in the interzone II-III region was mediated by the -1252/ + 10 VfENOD-GRP3 promoter region. This reporter gene expression in V. hirsuta was comparable to the location of VfENOD-GRP3 transcripts in V. faba nodules. An analysis of defined promoter fragments revealed that a strong gusAint expression in the interzone II-III region was also mediated by the -737/ + 10 promoter, whereas the -239/ + 10 promoter only mediated a weak gusAint expression in the interzone II-III region. Since the -239/ + 10 promoter fragment did not resemble published nodulin gene promoters, we propose that it contains new sequence motifs involved in mediating gene expression in the interzone II-III region of vicia nodules.
Keywords
- glycine-rich early nodulin, interzone II-III predominant expression, TL DNA integration vector, transgenic root nodules, Vicia hirsuta
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Plant molecular biology, Vol. 29, No. 4, 01.11.1995, p. 759-772.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The promoter of the Vicia faba L. VfENOD-GRP3 gene encoding a glycine-rich early nodulin mediates a predominant gene expression in the interzone II-III region of transgenic Vicia hirsuta root nodules
AU - Küster, Helge
AU - Quandt, Hans joachim
AU - Broer, Inge
AU - Perlick, Andreas M.
AU - Pühler, Alfred
PY - 1995/11/1
Y1 - 1995/11/1
N2 - We recently reported on the broad bean gene VfENOD-GRP3 encoding a glycine-rich early nodulin. This gene was predominantly expressed in the interzone II-III region of Vicia faba root nodules. The VfENOD-GRP3 promoter contained several sequence motifs potentially involved in the regulation of gene expression. To investigate the molecular basis for the specific VfENOD-GRP3 expression, defined VfENOD-GRP3 promoter fragments were fused to an intron-containing gusAint gene. Agrobacterium rhizogenes ARqual strains carrying these fusions integrated into the TL DNA were used to generate hairy roots on Vicia hirsuta, which subsequently were nodulated. Histochemical analysis of transgenic nodules indicated that a strong gusAint expression in the interzone II-III region was mediated by the -1252/ + 10 VfENOD-GRP3 promoter region. This reporter gene expression in V. hirsuta was comparable to the location of VfENOD-GRP3 transcripts in V. faba nodules. An analysis of defined promoter fragments revealed that a strong gusAint expression in the interzone II-III region was also mediated by the -737/ + 10 promoter, whereas the -239/ + 10 promoter only mediated a weak gusAint expression in the interzone II-III region. Since the -239/ + 10 promoter fragment did not resemble published nodulin gene promoters, we propose that it contains new sequence motifs involved in mediating gene expression in the interzone II-III region of vicia nodules.
AB - We recently reported on the broad bean gene VfENOD-GRP3 encoding a glycine-rich early nodulin. This gene was predominantly expressed in the interzone II-III region of Vicia faba root nodules. The VfENOD-GRP3 promoter contained several sequence motifs potentially involved in the regulation of gene expression. To investigate the molecular basis for the specific VfENOD-GRP3 expression, defined VfENOD-GRP3 promoter fragments were fused to an intron-containing gusAint gene. Agrobacterium rhizogenes ARqual strains carrying these fusions integrated into the TL DNA were used to generate hairy roots on Vicia hirsuta, which subsequently were nodulated. Histochemical analysis of transgenic nodules indicated that a strong gusAint expression in the interzone II-III region was mediated by the -1252/ + 10 VfENOD-GRP3 promoter region. This reporter gene expression in V. hirsuta was comparable to the location of VfENOD-GRP3 transcripts in V. faba nodules. An analysis of defined promoter fragments revealed that a strong gusAint expression in the interzone II-III region was also mediated by the -737/ + 10 promoter, whereas the -239/ + 10 promoter only mediated a weak gusAint expression in the interzone II-III region. Since the -239/ + 10 promoter fragment did not resemble published nodulin gene promoters, we propose that it contains new sequence motifs involved in mediating gene expression in the interzone II-III region of vicia nodules.
KW - glycine-rich early nodulin
KW - interzone II-III predominant expression
KW - TL DNA integration vector
KW - transgenic root nodules
KW - Vicia hirsuta
UR - http://www.scopus.com/inward/record.url?scp=0029410742&partnerID=8YFLogxK
U2 - 10.1007/BF00041166
DO - 10.1007/BF00041166
M3 - Article
C2 - 8541502
AN - SCOPUS:0029410742
VL - 29
SP - 759
EP - 772
JO - Plant molecular biology
JF - Plant molecular biology
SN - 0167-4412
IS - 4
ER -