Details
| Original language | English |
|---|---|
| Pages (from-to) | 225-233 |
| Journal | Journal of Virological Methods |
| Volume | 106 |
| Issue number | 2 |
| Publication status | Published - 2002 |
Abstract
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In: Journal of Virological Methods, Vol. 106, No. 2, 2002, p. 225-233.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The production of a genus-specific recombinant antibody (scFv) using a recombinant potyvirus protease
AU - Reinard, Thomas
AU - Maiß, Edgar
AU - Hust, Michael
N1 - Funding information: The generous gift of virus-infected plants from Dr. Vetten, BBA Braunschweig, Germany, is gratefully acknowledged. We thank Imdadul Hoque for helpful comments on the manuscript. This work is supported by the Ministry of Science and Culture (MWK) of the state Lower Saxony.
PY - 2002
Y1 - 2002
N2 - A single chain variable fragment antibody (scFv; anti-NIa scFv102) was selected from a synthetic human antibody library by using a NIa protease of Plum pox virus (PPV) as an antigen, which was expressed in bacteria. The NIa protease forms the nuclear inclusion body A and acts as the major protease in the cleavage of the viral polyprotein into functional proteins. The NIa protein was detected with anti-NIa scFv102 after expression in Escherichia coli cells as well as from PPV-infected Nicotiana benthamiana plants. Furthermore, the scFv102 has the ability to identify not only PPV from infected plants but also can detect other infections with members of the potyviruses. Nineteen different potyviruses were recognized by the scFv102 in various infected plants tested through dot blot assays. Therefore, the antibody scFv102 has the potential of becoming a general tool to detect potyvirus infections in different plant species. ?? 2002 Published by Elsevier Science B.V.
AB - A single chain variable fragment antibody (scFv; anti-NIa scFv102) was selected from a synthetic human antibody library by using a NIa protease of Plum pox virus (PPV) as an antigen, which was expressed in bacteria. The NIa protease forms the nuclear inclusion body A and acts as the major protease in the cleavage of the viral polyprotein into functional proteins. The NIa protein was detected with anti-NIa scFv102 after expression in Escherichia coli cells as well as from PPV-infected Nicotiana benthamiana plants. Furthermore, the scFv102 has the ability to identify not only PPV from infected plants but also can detect other infections with members of the potyviruses. Nineteen different potyviruses were recognized by the scFv102 in various infected plants tested through dot blot assays. Therefore, the antibody scFv102 has the potential of becoming a general tool to detect potyvirus infections in different plant species. ?? 2002 Published by Elsevier Science B.V.
U2 - 10.1016/S0166-0934(02)00166-0
DO - 10.1016/S0166-0934(02)00166-0
M3 - Article
VL - 106
SP - 225
EP - 233
JO - Journal of Virological Methods
JF - Journal of Virological Methods
SN - 0166-0934
IS - 2
ER -