Details
| Original language | English |
|---|---|
| Pages (from-to) | 582-585 |
| Number of pages | 4 |
| Journal | Applied Microbiology and Biotechnology |
| Volume | 34 |
| Issue number | 5 |
| Publication status | Published - Feb 1991 |
Abstract
Klebsiella oxytoca fermented 199 g·l-1 high test or invert molasses using batch fermentation with substrate shift to produce 95.2-98.6 g 2,3-butanediol·l-1 and 2,4-4.3 g acetoin·l-1 with a diol yield of 96-100% of the theoretical value and a diol productivity of 1.0-1.1 g·l-1·h-1. Fermentation was performed numerous times with molasses in repeated batch culture with cell recovery. Such repeated batch fermentation, in addition to a high product yield, also showed a very high product concentration. For example, 118 g 2,3-butanediol·l-1 and 2.3 g acetoin·l-1 were produced from 280 g·l-1 of high test molasses. The diol productivity in this fermentation amounted to 2.4 g·l-1·h-1 and can undoubtedly be further increased by increasing the cell concentration. Because the Klebsiella cultures ferment 2,3-butanediol at an extremely high rate once the sugar has been consumed, the culture was inhibited completely by the addition of 15 g ethanol·l-1 and switching off aeration.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biotechnology
- Immunology and Microbiology(all)
- Applied Microbiology and Biotechnology
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In: Applied Microbiology and Biotechnology, Vol. 34, No. 5, 02.1991, p. 582-585.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The production of 2,3-butanediol by fermentation of high test molasses
AU - Afschar, A. S.
AU - Bellgardt, K. H.
AU - Vaz Rossell, C. E.
AU - Czok, A.
AU - Schaller, K.
PY - 1991/2
Y1 - 1991/2
N2 - Klebsiella oxytoca fermented 199 g·l-1 high test or invert molasses using batch fermentation with substrate shift to produce 95.2-98.6 g 2,3-butanediol·l-1 and 2,4-4.3 g acetoin·l-1 with a diol yield of 96-100% of the theoretical value and a diol productivity of 1.0-1.1 g·l-1·h-1. Fermentation was performed numerous times with molasses in repeated batch culture with cell recovery. Such repeated batch fermentation, in addition to a high product yield, also showed a very high product concentration. For example, 118 g 2,3-butanediol·l-1 and 2.3 g acetoin·l-1 were produced from 280 g·l-1 of high test molasses. The diol productivity in this fermentation amounted to 2.4 g·l-1·h-1 and can undoubtedly be further increased by increasing the cell concentration. Because the Klebsiella cultures ferment 2,3-butanediol at an extremely high rate once the sugar has been consumed, the culture was inhibited completely by the addition of 15 g ethanol·l-1 and switching off aeration.
AB - Klebsiella oxytoca fermented 199 g·l-1 high test or invert molasses using batch fermentation with substrate shift to produce 95.2-98.6 g 2,3-butanediol·l-1 and 2,4-4.3 g acetoin·l-1 with a diol yield of 96-100% of the theoretical value and a diol productivity of 1.0-1.1 g·l-1·h-1. Fermentation was performed numerous times with molasses in repeated batch culture with cell recovery. Such repeated batch fermentation, in addition to a high product yield, also showed a very high product concentration. For example, 118 g 2,3-butanediol·l-1 and 2.3 g acetoin·l-1 were produced from 280 g·l-1 of high test molasses. The diol productivity in this fermentation amounted to 2.4 g·l-1·h-1 and can undoubtedly be further increased by increasing the cell concentration. Because the Klebsiella cultures ferment 2,3-butanediol at an extremely high rate once the sugar has been consumed, the culture was inhibited completely by the addition of 15 g ethanol·l-1 and switching off aeration.
UR - http://www.scopus.com/inward/record.url?scp=0025972617&partnerID=8YFLogxK
U2 - 10.1007/BF00167903
DO - 10.1007/BF00167903
M3 - Article
AN - SCOPUS:0025972617
VL - 34
SP - 582
EP - 585
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
IS - 5
ER -