Details
| Original language | English |
|---|---|
| Pages (from-to) | 573-87 |
| Number of pages | 15 |
| Journal | PLANTA |
| Volume | 228 |
| Issue number | 4 |
| Publication status | Published - Sept 2008 |
Abstract
The long-term response (LTR) of higher plants to varying light qualities increases the photosynthetic yield; however, the benefit of this improvement for physiology and survival of plants is largely unknown, and its functional relation to other light acclimation responses has never been investigated. To unravel positive effects of the LTR we acclimated Arabidopsis thaliana for several days to light sources, which preferentially excite photosystem I (PSI) or photosystem II (PSII). After acclimation, plants revealed characteristic differences in chlorophyll fluorescence, thylakoid membrane stacking, phosphorylation state of PSII subunits and photosynthetic yield of PSII and PSI. These LTR-induced changes in the structure, function and efficiency of the photosynthetic machinery are true effects by light quality acclimation, which could not be induced by light intensity variations in the low light range. In addition, high light stress experiments indicated that the LTR is not involved in photoinhibition; however, it lowers non-photochemical quenching (NPQ) by directing more absorbed light energy into photochemical work. NPQ in turn is not essential for the LTR, since npq mutants performed a normal acclimation. We quantified the beneficial potential of the LTR by comparing wild-type plants with the LTR-deficient mutant stn7. The mutant exhibited a decreased effective quantum yield and produced only half of seeds when grown under fluctuating light quality conditions. Thus, the LTR represents a distinct acclimation response in addition to other already known responses that clearly improves plant physiology under low light conditions resulting in a pronounced positive effect on plant fitness.
Keywords
- Acclimatization, Arabidopsis/genetics, Chloroplasts/physiology, Fertility, Fluorescence, Mutation, Photosynthesis/radiation effects, Photosystem I Protein Complex/analysis, Photosystem II Protein Complex/analysis, Reaction Time, Seedlings, Thylakoids/ultrastructure
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In: PLANTA, Vol. 228, No. 4, 09.2008, p. 573-87.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The long-term response to fluctuating light quality is an important and distinct light acclimation mechanism that supports survival of Arabidopsis thaliana under low light conditions
AU - Wagner, Raik
AU - Dietzel, Lars
AU - Bräutigam, Katharina
AU - Fischer, Wolfgang
AU - Pfannschmidt, Thomas
N1 - Funding information: Acknowledgments This work was supported by grants from the DFG to TP and the DFG research groups FOR 387, FOR 804 and by the NWP programme of Thuringia. Meta Brost and Nicole Manthei are acknowledged for their skilful technical assistance.
PY - 2008/9
Y1 - 2008/9
N2 - The long-term response (LTR) of higher plants to varying light qualities increases the photosynthetic yield; however, the benefit of this improvement for physiology and survival of plants is largely unknown, and its functional relation to other light acclimation responses has never been investigated. To unravel positive effects of the LTR we acclimated Arabidopsis thaliana for several days to light sources, which preferentially excite photosystem I (PSI) or photosystem II (PSII). After acclimation, plants revealed characteristic differences in chlorophyll fluorescence, thylakoid membrane stacking, phosphorylation state of PSII subunits and photosynthetic yield of PSII and PSI. These LTR-induced changes in the structure, function and efficiency of the photosynthetic machinery are true effects by light quality acclimation, which could not be induced by light intensity variations in the low light range. In addition, high light stress experiments indicated that the LTR is not involved in photoinhibition; however, it lowers non-photochemical quenching (NPQ) by directing more absorbed light energy into photochemical work. NPQ in turn is not essential for the LTR, since npq mutants performed a normal acclimation. We quantified the beneficial potential of the LTR by comparing wild-type plants with the LTR-deficient mutant stn7. The mutant exhibited a decreased effective quantum yield and produced only half of seeds when grown under fluctuating light quality conditions. Thus, the LTR represents a distinct acclimation response in addition to other already known responses that clearly improves plant physiology under low light conditions resulting in a pronounced positive effect on plant fitness.
AB - The long-term response (LTR) of higher plants to varying light qualities increases the photosynthetic yield; however, the benefit of this improvement for physiology and survival of plants is largely unknown, and its functional relation to other light acclimation responses has never been investigated. To unravel positive effects of the LTR we acclimated Arabidopsis thaliana for several days to light sources, which preferentially excite photosystem I (PSI) or photosystem II (PSII). After acclimation, plants revealed characteristic differences in chlorophyll fluorescence, thylakoid membrane stacking, phosphorylation state of PSII subunits and photosynthetic yield of PSII and PSI. These LTR-induced changes in the structure, function and efficiency of the photosynthetic machinery are true effects by light quality acclimation, which could not be induced by light intensity variations in the low light range. In addition, high light stress experiments indicated that the LTR is not involved in photoinhibition; however, it lowers non-photochemical quenching (NPQ) by directing more absorbed light energy into photochemical work. NPQ in turn is not essential for the LTR, since npq mutants performed a normal acclimation. We quantified the beneficial potential of the LTR by comparing wild-type plants with the LTR-deficient mutant stn7. The mutant exhibited a decreased effective quantum yield and produced only half of seeds when grown under fluctuating light quality conditions. Thus, the LTR represents a distinct acclimation response in addition to other already known responses that clearly improves plant physiology under low light conditions resulting in a pronounced positive effect on plant fitness.
KW - Acclimatization
KW - Arabidopsis/genetics
KW - Chloroplasts/physiology
KW - Fertility
KW - Fluorescence
KW - Mutation
KW - Photosynthesis/radiation effects
KW - Photosystem I Protein Complex/analysis
KW - Photosystem II Protein Complex/analysis
KW - Reaction Time
KW - Seedlings
KW - Thylakoids/ultrastructure
U2 - 10.1007/s00425-008-0760-y
DO - 10.1007/s00425-008-0760-y
M3 - Article
C2 - 18542996
VL - 228
SP - 573
EP - 587
JO - PLANTA
JF - PLANTA
SN - 0032-0935
IS - 4
ER -