Details
| Original language | English |
|---|---|
| Pages (from-to) | 359-373 |
| Number of pages | 15 |
| Journal | Journal of plant biology |
| Volume | 62 |
| Issue number | 5 |
| Early online date | 3 Oct 2019 |
| Publication status | Published - Oct 2019 |
Abstract
Adapting biological processes to an endogenous rhythm enables plants to cope with the daily changes in light and temperature in a more predictable way enhancing growth and fitness. A number of biological processes such as metabolic pathways as well as the immunity in plants are under diurnal or circadian control. In this study a possible circadian regulation of key enzymes in the sulfur assimilation and the corresponding metabolites was investigated in the agriculturally important crop plant oilseed rape (Brassica napus). Leaves of a commercially available cultivar were harvested in the course of a day under diurnal and under free-running conditions with constant light. Analyses in this study were focused on sulfur-containing metabolites and expression analysis of enzymes involved in sulfur assimilation. Expression analysis showed that the transcript levels of the sulfate transporters Sultr3;1 and Sultr4;2 as well as APR2 and APR3 oscillated diurnally. Results revealed a periodic rhythm of sulfur-containing metabolites such as glutathione, sulfate and certain glucosinolates in the course of a day which were only partly maintained under constant light. Therefore, we conclude that a diurnal rhythm and not the circadian clock regulates sulfur metabolism in plants.
Keywords
- Brassica napus, Circadian clock, Diurnal rhythm, Expression analysis, Sulfur-containing metabolites
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Plant Science
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In: Journal of plant biology, Vol. 62, No. 5, 10.2019, p. 359-373.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The Levels of Sulfur-containing Metabolites in Brassica napus are Not Influenced by the Circadian Clock but Diurnally
AU - Hornbacher, Johann
AU - Rumlow, Annekathrin
AU - Pallmann, Philip
AU - Turcios, Ariel E.
AU - Riemenschneider, Anja
AU - Papenbrock, Jutta
N1 - Funding information: We acknowledge the Deutsche Saatveredelung AG, Lippstadt, Germany, for providing us with seeds of the Brassica napus cultivar. We would like to thank Julia Volker and Pamela von Trzebiatowski, Hannover, for their excellent technical assistance and the gardener Yvonne Leye for taking care of the plants. We gratefully acknowledge Dr. Elke Bloem, Julius-Kühn-Institut, Germany, who analyzed the samples for their sulfate content.
PY - 2019/10
Y1 - 2019/10
N2 - Adapting biological processes to an endogenous rhythm enables plants to cope with the daily changes in light and temperature in a more predictable way enhancing growth and fitness. A number of biological processes such as metabolic pathways as well as the immunity in plants are under diurnal or circadian control. In this study a possible circadian regulation of key enzymes in the sulfur assimilation and the corresponding metabolites was investigated in the agriculturally important crop plant oilseed rape (Brassica napus). Leaves of a commercially available cultivar were harvested in the course of a day under diurnal and under free-running conditions with constant light. Analyses in this study were focused on sulfur-containing metabolites and expression analysis of enzymes involved in sulfur assimilation. Expression analysis showed that the transcript levels of the sulfate transporters Sultr3;1 and Sultr4;2 as well as APR2 and APR3 oscillated diurnally. Results revealed a periodic rhythm of sulfur-containing metabolites such as glutathione, sulfate and certain glucosinolates in the course of a day which were only partly maintained under constant light. Therefore, we conclude that a diurnal rhythm and not the circadian clock regulates sulfur metabolism in plants.
AB - Adapting biological processes to an endogenous rhythm enables plants to cope with the daily changes in light and temperature in a more predictable way enhancing growth and fitness. A number of biological processes such as metabolic pathways as well as the immunity in plants are under diurnal or circadian control. In this study a possible circadian regulation of key enzymes in the sulfur assimilation and the corresponding metabolites was investigated in the agriculturally important crop plant oilseed rape (Brassica napus). Leaves of a commercially available cultivar were harvested in the course of a day under diurnal and under free-running conditions with constant light. Analyses in this study were focused on sulfur-containing metabolites and expression analysis of enzymes involved in sulfur assimilation. Expression analysis showed that the transcript levels of the sulfate transporters Sultr3;1 and Sultr4;2 as well as APR2 and APR3 oscillated diurnally. Results revealed a periodic rhythm of sulfur-containing metabolites such as glutathione, sulfate and certain glucosinolates in the course of a day which were only partly maintained under constant light. Therefore, we conclude that a diurnal rhythm and not the circadian clock regulates sulfur metabolism in plants.
KW - Brassica napus
KW - Circadian clock
KW - Diurnal rhythm
KW - Expression analysis
KW - Sulfur-containing metabolites
UR - http://www.scopus.com/inward/record.url?scp=85073252554&partnerID=8YFLogxK
U2 - 10.1007/s12374-019-0143-x
DO - 10.1007/s12374-019-0143-x
M3 - Article
AN - SCOPUS:85073252554
VL - 62
SP - 359
EP - 373
JO - Journal of plant biology
JF - Journal of plant biology
SN - 1226-9239
IS - 5
ER -