Details
| Original language | English |
|---|---|
| Pages (from-to) | 445-461 |
| Number of pages | 17 |
| Journal | European Journal of Plant Pathology |
| Volume | 141 |
| Issue number | 3 |
| Publication status | Published - Mar 2015 |
Abstract
A well-established population of Claviceps purpurea var. spartinae R.A. Duncan & J.F. White was found on the invasive common cord-grass Spartina anglica C.E. Hubbard at two localities on the German North Sea coast. Additionally, 25 populations of S. anglica with an average air-line distance of 50 km between them along the coastline, starting from the Netherlands via the German coastline up to Denmark, were investigated. All S. anglica populations were heavily infected with C. purpurea var. spartinae (lineage G3). Genetic characterization of the C. purpurea DNA using Amplified Fragment Length Polymorphism (AFLP) and Internal Transcribed Spacer (ITS) analysis clearly indicated that all populations collected at the Wadden Sea were grouped in the same epidemic clade. Comparisons with reference strains indicated that the isolates along the Wadden Sea belonged to the clade also found in Ireland and Wales. The ergot alkaloid pattern is very similar in all genotypes. Ergocristine, α-ergocryptine and their epimers were identified in the samples in high amounts comparable to average values in sclerotia found on rye. The sclerotia of C. purpurea var. spartinae are able to float on the water for several months and could thereby be distributed over large distances by seawater. The sclerotia found in the drift lines still contain the equally high contents of ergot alkaloids as those collected directly from the plants. Current data form the basis for risk assessments and predictions about further spreading of C. purpurea var. spartinae on S. anglica.
Keywords
- Amplified fragment length polymorphism (AFLP), Claviceps purpurea var. spartinae, Epimers, Ergot alkaloids, Internal transcribed spacer (ITS), Invasive species, Spartina anglica, Wadden Sea
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Agronomy and Crop Science
- Agricultural and Biological Sciences(all)
- Plant Science
- Agricultural and Biological Sciences(all)
- Horticulture
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In: European Journal of Plant Pathology, Vol. 141, No. 3, 03.2015, p. 445-461.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The invasive ergot Claviceps purpurea var. spartinae recently established in the European Wadden Sea on common cord grass is genetically homogeneous and the sclerotia contain high amounts of ergot alkaloids
AU - Boestfleisch, Christian
AU - Drotleff, Astrid M.
AU - Ternes, Waldemar
AU - Nehring, Stefan
AU - Pažoutová, Sylvie
AU - Papenbrock, Jutta
N1 - Publisher Copyright: © 2014, Koninklijke Nederlandse Planteziektenkundige Vereniging. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2015/3
Y1 - 2015/3
N2 - A well-established population of Claviceps purpurea var. spartinae R.A. Duncan & J.F. White was found on the invasive common cord-grass Spartina anglica C.E. Hubbard at two localities on the German North Sea coast. Additionally, 25 populations of S. anglica with an average air-line distance of 50 km between them along the coastline, starting from the Netherlands via the German coastline up to Denmark, were investigated. All S. anglica populations were heavily infected with C. purpurea var. spartinae (lineage G3). Genetic characterization of the C. purpurea DNA using Amplified Fragment Length Polymorphism (AFLP) and Internal Transcribed Spacer (ITS) analysis clearly indicated that all populations collected at the Wadden Sea were grouped in the same epidemic clade. Comparisons with reference strains indicated that the isolates along the Wadden Sea belonged to the clade also found in Ireland and Wales. The ergot alkaloid pattern is very similar in all genotypes. Ergocristine, α-ergocryptine and their epimers were identified in the samples in high amounts comparable to average values in sclerotia found on rye. The sclerotia of C. purpurea var. spartinae are able to float on the water for several months and could thereby be distributed over large distances by seawater. The sclerotia found in the drift lines still contain the equally high contents of ergot alkaloids as those collected directly from the plants. Current data form the basis for risk assessments and predictions about further spreading of C. purpurea var. spartinae on S. anglica.
AB - A well-established population of Claviceps purpurea var. spartinae R.A. Duncan & J.F. White was found on the invasive common cord-grass Spartina anglica C.E. Hubbard at two localities on the German North Sea coast. Additionally, 25 populations of S. anglica with an average air-line distance of 50 km between them along the coastline, starting from the Netherlands via the German coastline up to Denmark, were investigated. All S. anglica populations were heavily infected with C. purpurea var. spartinae (lineage G3). Genetic characterization of the C. purpurea DNA using Amplified Fragment Length Polymorphism (AFLP) and Internal Transcribed Spacer (ITS) analysis clearly indicated that all populations collected at the Wadden Sea were grouped in the same epidemic clade. Comparisons with reference strains indicated that the isolates along the Wadden Sea belonged to the clade also found in Ireland and Wales. The ergot alkaloid pattern is very similar in all genotypes. Ergocristine, α-ergocryptine and their epimers were identified in the samples in high amounts comparable to average values in sclerotia found on rye. The sclerotia of C. purpurea var. spartinae are able to float on the water for several months and could thereby be distributed over large distances by seawater. The sclerotia found in the drift lines still contain the equally high contents of ergot alkaloids as those collected directly from the plants. Current data form the basis for risk assessments and predictions about further spreading of C. purpurea var. spartinae on S. anglica.
KW - Amplified fragment length polymorphism (AFLP)
KW - Claviceps purpurea var. spartinae
KW - Epimers
KW - Ergot alkaloids
KW - Internal transcribed spacer (ITS)
KW - Invasive species
KW - Spartina anglica
KW - Wadden Sea
UR - http://www.scopus.com/inward/record.url?scp=84925543165&partnerID=8YFLogxK
U2 - 10.1007/s10658-014-0555-x
DO - 10.1007/s10658-014-0555-x
M3 - Article
AN - SCOPUS:84925543165
VL - 141
SP - 445
EP - 461
JO - European Journal of Plant Pathology
JF - European Journal of Plant Pathology
SN - 0929-1873
IS - 3
ER -