Details
| Original language | English |
|---|---|
| Pages (from-to) | 1423-1441 |
| Number of pages | 19 |
| Journal | Food & function |
| Volume | 16 |
| Issue number | 4 |
| Early online date | 3 Feb 2025 |
| Publication status | Published - 2025 |
Abstract
In recent years, the interest of consumers in fruit juice extracts as nutraceuticals has increased. Fruits, especially red berries, contain valuable bioactive compounds such as polyphenols. Polyphenols are often associated with anti-oxidant, anti-inflammatory, anti-diabetic, anti-cancer, cardioprotective and gastroprotective properties. However, the relationship between the various effects of fruit juice extracts and their influence on the permeability of the intestinal barrier, as well as their influence on glucose transport across the intestinal membrane, is not known. Therefore, in the present study, anthocyanins and copigments were obtained from 11 fruit juice extracts by XAD7 column chromatography and characterized their health-promoting effects, as well as their influence on the intestinal membrane. Chokeberry, pomegranate and blueberry extracts showed the highest antioxidant activity, but showed incomplete regeneration of the intestinal membrane upon treatment-induced higher permeability. This may depended on the high anthocyanin level of these extracts. Treatments with gojiberry extract, elderberry extract and the copigment fraction of apple achieved the best suitable regeneration of the intestinal barrier. The transcription of epithelial glucose transporters GLUT1 und GLUT2 as well as for the oxidative stress genes catalase (CAT) and superoxide dismutase (SOD) were most effectively reduced by chokeberry extract. To sum up, fruit juice extracts possess high antioxidant potentials and can reduce the expression of antioxidant enzymes and glucose transporters in colon cells. While the glucose uptake may be reduced, the intestinal permeability is increased, which varies due to the extract composition. Therefore, fruit juice extracts need to be fractionated and characterized in more detail to identify the health-beneficial compounds.
Sustainable Development Goals
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In: Food & function, Vol. 16, No. 4, 2025, p. 1423-1441.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The influence of fruit juice extracts on glucose intestinal transporters and antioxidant genes in a Caco-2 and HT29-MTX co-culture cell system
AU - Köpsel, Magdalena
AU - Kostka, Tina
AU - Rodriguez-Werner, Miriam
AU - Esatbeyoglu, Tuba
PY - 2025
Y1 - 2025
N2 - In recent years, the interest of consumers in fruit juice extracts as nutraceuticals has increased. Fruits, especially red berries, contain valuable bioactive compounds such as polyphenols. Polyphenols are often associated with anti-oxidant, anti-inflammatory, anti-diabetic, anti-cancer, cardioprotective and gastroprotective properties. However, the relationship between the various effects of fruit juice extracts and their influence on the permeability of the intestinal barrier, as well as their influence on glucose transport across the intestinal membrane, is not known. Therefore, in the present study, anthocyanins and copigments were obtained from 11 fruit juice extracts by XAD7 column chromatography and characterized their health-promoting effects, as well as their influence on the intestinal membrane. Chokeberry, pomegranate and blueberry extracts showed the highest antioxidant activity, but showed incomplete regeneration of the intestinal membrane upon treatment-induced higher permeability. This may depended on the high anthocyanin level of these extracts. Treatments with gojiberry extract, elderberry extract and the copigment fraction of apple achieved the best suitable regeneration of the intestinal barrier. The transcription of epithelial glucose transporters GLUT1 und GLUT2 as well as for the oxidative stress genes catalase (CAT) and superoxide dismutase (SOD) were most effectively reduced by chokeberry extract. To sum up, fruit juice extracts possess high antioxidant potentials and can reduce the expression of antioxidant enzymes and glucose transporters in colon cells. While the glucose uptake may be reduced, the intestinal permeability is increased, which varies due to the extract composition. Therefore, fruit juice extracts need to be fractionated and characterized in more detail to identify the health-beneficial compounds.
AB - In recent years, the interest of consumers in fruit juice extracts as nutraceuticals has increased. Fruits, especially red berries, contain valuable bioactive compounds such as polyphenols. Polyphenols are often associated with anti-oxidant, anti-inflammatory, anti-diabetic, anti-cancer, cardioprotective and gastroprotective properties. However, the relationship between the various effects of fruit juice extracts and their influence on the permeability of the intestinal barrier, as well as their influence on glucose transport across the intestinal membrane, is not known. Therefore, in the present study, anthocyanins and copigments were obtained from 11 fruit juice extracts by XAD7 column chromatography and characterized their health-promoting effects, as well as their influence on the intestinal membrane. Chokeberry, pomegranate and blueberry extracts showed the highest antioxidant activity, but showed incomplete regeneration of the intestinal membrane upon treatment-induced higher permeability. This may depended on the high anthocyanin level of these extracts. Treatments with gojiberry extract, elderberry extract and the copigment fraction of apple achieved the best suitable regeneration of the intestinal barrier. The transcription of epithelial glucose transporters GLUT1 und GLUT2 as well as for the oxidative stress genes catalase (CAT) and superoxide dismutase (SOD) were most effectively reduced by chokeberry extract. To sum up, fruit juice extracts possess high antioxidant potentials and can reduce the expression of antioxidant enzymes and glucose transporters in colon cells. While the glucose uptake may be reduced, the intestinal permeability is increased, which varies due to the extract composition. Therefore, fruit juice extracts need to be fractionated and characterized in more detail to identify the health-beneficial compounds.
UR - http://www.scopus.com/inward/record.url?scp=85216869012&partnerID=8YFLogxK
U2 - 10.1039/d4fo03950e
DO - 10.1039/d4fo03950e
M3 - Article
VL - 16
SP - 1423
EP - 1441
JO - Food & function
JF - Food & function
SN - 2042-6496
IS - 4
ER -