Details
| Original language | English |
|---|---|
| Pages (from-to) | 278-294 |
| Number of pages | 17 |
| Journal | Plant Journal |
| Volume | 109 |
| Issue number | 1 |
| Early online date | 29 Oct 2021 |
| Publication status | Published - Dec 2021 |
Abstract
European mistletoe is a hemiparasitic flowering plant that is known for a very special life cycle and extraordinary biochemical properties. Particularly, V. album has an unusual mode of cellular respiration that takes place in the absence of mitochondrial complex I. However, insights into the molecular biology of V. album so far are very limited. Since the genome of V. album is extremely large (estimated 600 times larger than the genome of the model plant Arabidopsis thaliana) it has not been sequenced up to now. We here report sequencing of the V. album gene space (defined as the space including and surrounding genic regions, encompassing coding as well as 5' and 3' non-coding regions). Messenger-RNA fractions were isolated from different V. album organs harvested in summer or winter and were analysed via Single Molecule Real-Time sequencing. We determined sequences of 39,092 distinct open reading frames encoding 32,064 V. album proteins (designated V. album protein space). Our data give new insights into the metabolism and molecular biology of V. album, including the biosynthesis of lectins and viscotoxins. The benefits of the V. album gene space information are demonstrated by re-evaluating mass spectrometry based data of the V. album mitochondrial proteome, which previously had been evaluated using the Arabidopsis thaliana genome sequence. Our re-examination allowed the additional identification of nearly 200 mitochondrial proteins, including four proteins related to complex I, which all have a secondary function not related to respiratory electron transport. The V. album gene space sequences are available at the NCBI.
Keywords
- Arabidopsis thaliana, SMRT sequencing, Viscum album, complex I, lectins, mitochondria, oxidative phosphorylation, viscotoxins
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Genetics
- Agricultural and Biological Sciences(all)
- Plant Science
- Biochemistry, Genetics and Molecular Biology(all)
- Cell Biology
Sustainable Development Goals
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In: Plant Journal, Vol. 109, No. 1, 12.2021, p. 278-294.
Research output: Contribution to journal › Article › Research › peer review
}
TY - JOUR
T1 - The gene space of European mistletoe (Viscum album)
AU - Schröder, Lucie
AU - Hohnjec, Natalija
AU - Senkler, Michael
AU - Senkler, Jennifer
AU - Küster, Helge
AU - Braun, Hans-Peter
N1 - Funding Information: We thank Dr. Sabine Reichwein for project advice, Marius Schrader for taking care of the apple tree used for cultivation and Matthias Döring for critical reading of the manuscript. PacBio SMRT sequencing was carried out by Novogene, Cambridge, UK. This research has been supported by the Deutsche Forschungsgemeinschaft (grant BR 1829/16‐1 to HPB). Open access funding enabled and organized by Projekt DEAL. V. album
PY - 2021/12
Y1 - 2021/12
N2 - European mistletoe is a hemiparasitic flowering plant that is known for a very special life cycle and extraordinary biochemical properties. Particularly, V. album has an unusual mode of cellular respiration that takes place in the absence of mitochondrial complex I. However, insights into the molecular biology of V. album so far are very limited. Since the genome of V. album is extremely large (estimated 600 times larger than the genome of the model plant Arabidopsis thaliana) it has not been sequenced up to now. We here report sequencing of the V. album gene space (defined as the space including and surrounding genic regions, encompassing coding as well as 5' and 3' non-coding regions). Messenger-RNA fractions were isolated from different V. album organs harvested in summer or winter and were analysed via Single Molecule Real-Time sequencing. We determined sequences of 39,092 distinct open reading frames encoding 32,064 V. album proteins (designated V. album protein space). Our data give new insights into the metabolism and molecular biology of V. album, including the biosynthesis of lectins and viscotoxins. The benefits of the V. album gene space information are demonstrated by re-evaluating mass spectrometry based data of the V. album mitochondrial proteome, which previously had been evaluated using the Arabidopsis thaliana genome sequence. Our re-examination allowed the additional identification of nearly 200 mitochondrial proteins, including four proteins related to complex I, which all have a secondary function not related to respiratory electron transport. The V. album gene space sequences are available at the NCBI.
AB - European mistletoe is a hemiparasitic flowering plant that is known for a very special life cycle and extraordinary biochemical properties. Particularly, V. album has an unusual mode of cellular respiration that takes place in the absence of mitochondrial complex I. However, insights into the molecular biology of V. album so far are very limited. Since the genome of V. album is extremely large (estimated 600 times larger than the genome of the model plant Arabidopsis thaliana) it has not been sequenced up to now. We here report sequencing of the V. album gene space (defined as the space including and surrounding genic regions, encompassing coding as well as 5' and 3' non-coding regions). Messenger-RNA fractions were isolated from different V. album organs harvested in summer or winter and were analysed via Single Molecule Real-Time sequencing. We determined sequences of 39,092 distinct open reading frames encoding 32,064 V. album proteins (designated V. album protein space). Our data give new insights into the metabolism and molecular biology of V. album, including the biosynthesis of lectins and viscotoxins. The benefits of the V. album gene space information are demonstrated by re-evaluating mass spectrometry based data of the V. album mitochondrial proteome, which previously had been evaluated using the Arabidopsis thaliana genome sequence. Our re-examination allowed the additional identification of nearly 200 mitochondrial proteins, including four proteins related to complex I, which all have a secondary function not related to respiratory electron transport. The V. album gene space sequences are available at the NCBI.
KW - Arabidopsis thaliana
KW - SMRT sequencing
KW - Viscum album
KW - complex I
KW - lectins
KW - mitochondria
KW - oxidative phosphorylation
KW - viscotoxins
UR - http://www.scopus.com/inward/record.url?scp=85119138581&partnerID=8YFLogxK
U2 - 10.1111/tpj.15558
DO - 10.1111/tpj.15558
M3 - Article
C2 - 34713513
VL - 109
SP - 278
EP - 294
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 1
ER -